Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Legume Research
Print ISSN: 0250-5371
Online ISSN: 0976-0571
NASS Rating
6.80
SJR
0.32
CiteScore
0.906

Chief Editor:
J. S. Sandhu
Vice Chancellor, SKN Agriculture, University, Jobner, VC, NDUAT, Faizabad, Deputy Director General (Crop Science), Indian Council of Agricultural Research (ICAR), New Delhi
Frequency:Monthly
Indexing:
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Go...

Legume Research, volume 36 issue 4 (august 2013) : 344-350

MOLECULAR DIVERSITY OF ROOT AND NODULE ENDOPHYTIC BACTERIA FROM FIELD PEA (PISUM SATIVUM L.)

Shifa Narula, R.C. Anand, S.S. Dudeja*, Vishal, D.V. Pathak1
1Department of Microbiology, CCS Haryana Agricultural University, Hisar- 125 004 India

  • Submitted|

  • First Online |

  • doi

Cite article:- Narula Shifa, Anand R.C., Dudeja* S.S., Vishal, Pathak1 D.V. (2025). MOLECULAR DIVERSITY OF ROOT AND NODULE ENDOPHYTIC BACTERIA FROM FIELD PEA (PISUM SATIVUM L.). Legume Research. 36(4): 344-350. doi: .

ABSTRACT

Endophytic bacteria from root and nodules of field pea being grown in CCS Haryana Agricultural University farm Hisar were isolated. A total of 15 endophytic bacteria from surface sterilized roots and 60 from the nodules of field pea were isolated. Out of 75 isolates, 67% in roots and 68% in nodules were gram positive. In roots 70% and in nodules 90% of the gram positive isolates was spore formers. Selected 9 endophytes from roots and 48 from nodules were used to determine molecular diversity by amplication of 16S rDNA by PCR, followed by RFLP by three restriction endonucleases Hae III, RsaI & Hinf I. Wide diversity among field pea bacterial endophytes was observed and considering each cluster as one genotype at 80% level of similarity coefficient,  thereby in CCS Haryana Agricultural University soils, 3 genotypes were present in field pea roots while 17 genotypes were present in the nodules. A combined dandrogram showed that entirely a separate cluster was formed by the root endophytes as compared to nodules endophytes, which indicates that the number and type of bacterial genera enters depending upon the plant tissue and environmental conditions in field pea, besides the microbial population present in the soil.

REFERENCES

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, Albright LM, Coen DM, Varki A, Chanda VB (eds.). (2001) Current Protocols in Molecular Biology. Vol. 1 Unit 2.4 New York: John Wiley.
  2. Deng ZS, Zhao LF, Kong ZY, Yang WQ, Lindström K, Wang ET, Wei GH (2011) Diversity of endophytic bacteria within nodules of the Sphaerophysa salsula in different regions of Loess Plateau in China. FEMS Microbiol Ecol 76(3): 463-475.
  3. Dudeja SS, Giri R, Saini R, Suneja-Madan P, Kothe E (2012) Interaction of endophytic microbes with legumes. J. Basic Microbiol. 52: Article first published online: 23 Sep, 2011, DOI: 10.1002/jobm.201100063.
  4. Dudeja SS, Narula N, Anand RC (2009) Plant Microbe Interactions – A practical manual for laboratory studies. Published by Director HRM, CCS Haryana Agricultural University . Hisar. 125 004. India. pp1-140
  5. Dudeja SS, Singh NP, Sharma P, Gupta SC, Chandra R, Dhar B, Bansal RK, Brahmaprakash GP, Potdukhe SR, Gundappagol RC, Gaikawad BG, Nagaraj KS (2011) Biofertilizer technology and productivity of chickpea in India. In: Bioaugmentation, biostimulation and biocontrol. Soil biology, 28. (Singh, A., Parmar, N. and Kuhad, R.C. (eds.)), Springer-Verlag Berlin Heidelberg. pp 43-63.
  6. Dudeja SS, Singh PC (2008). High and low nodulation in relation to molecular diversity of chickpea mesorhizobia in Indian soils. Arch Agron Soil Sci 54: 109-120.
  7. Elbeltagy A, Nishioka K, Suzuki H, Sato T, Sato YI, Morisaki H, Mitsui H, Minamisawa, K (2000) Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. Soil Sci Plant Nutr 46: 617-629.
  8. Hoque MS, Broadhurst LM, Thrall PH (2011) Genetic characterisation of root nodule bacteria associated with Acacia salicina and Acacia stenophylla (Mimosaceae) across south eastern Australia. Int. J Syst Evol Microbiol 61(2): 299-309.
  9. Hung PQ, Kumar SM, Govindsamy V, Annapurna K (2007) Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties. Biol Fertil Soils 44: 155-162.
  10. Li JH, Wang ET, Chen WF, Chen WX (2008) Genetic diversity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biol Biochem 40: 238- 246.
  11. Lukow T, Dunfield PF, Liesack W (2000) Use of the t-RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. FEMS Microbiol Ecol 32: 241–247.
  12. Muresu R, Polone E, Sorbolini S, Squartini A (2011) Characterization of endophytic and symbiotic bacteria within plants of the endemic association Centaureetum horridae. Mol. Plant Biosys 145(2): 478–484.
  13. Muresu R, Polone E, Sulas L, Baldan B, Tondello A, Delogu G, Cappuccinelli P, Alberghini S, Benhizia Y, Benhizia H, Benguedouar A, Mori B, Calamassi R, Dazzo FB, Squartini A (2008) Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes. FEMS Microbiol Ecol 63: 383-400.
  14. Palaniappan P, Chauhan PS, Saravanan VS, Anandham R, Sa T (2010) Isolation and characterization of plant growth promoting endophytic bacterial isolates from root nodule of Lespedeza sp. Biol Fertil Soils 46: 807-816.
  15. Pillay VK, Nowak J (1997) Inoculum density, temperature and genotype effects on epiphytic and endophytic colonization and in vitro growth promotion of tomato (Lycopersicon esculentum L.) by a pseudomonad bacterium. Can J Microbiol 43: 354-361.
  16. Rohlf FJ (1998) On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol 47: 147- 158.
  17. Stoltzfus JR, So R, Malarvithi PP, Ladha JK, De Bruijn FJ (1997) Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194: 25-36.
  18. Sturz AV, Christie BR, Matheson BG, Nowak J (1997) Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biol Fertil Soils 25: 13-19.
  19. Tak N, Gehlot HS, Ardley JK, Rathore MS, Tripathy AK, Shekhawat NS, Tiwari R, Reeve W, Sprent JI, Howieson J (2009) Endophytic bacterial diversity in root nodules of native legumes from Indian Thar Desert. In: 15th Australian Nitrogen Fixation Conference, Margaret River, Western Australia.
  20. Takeli AS, Ates E (2003) Yield and its components in field pea lines. J Cent Europ Agric 4: 313-317.
  21. Tan Z, Hurek T, Reinhold-Hurek B (2003) Effect of N-fertilization, plant genotype and environmental conditions on nifH gene pools in roots of rice. Environ Microbiol 5: 1009-1015.
  22. Vincent JM (1970) A manual for the practical study of root nodule bacteria. IBM Handbook No. 15. Oxford: Blackwell Scientific Publications.
  23. Wadhwa K, Dudeja SS, Yadav RK (2011) Molecular diversity of native field pea rhizobia trapped by five contrasting field pea genotypes in Indian soils. J Basic Microbiol 51: 89-97.
  24. Zakhia F, Jeder H, Domergue O, Willems A, Cleyet-Marel CJ, Gillis M, Dreyfus B, de Lajudie P (2006) Characterisation of wild legume nodulating bacteria (LNB) in the infra-arid zone of Tunisia. Syst Appl Microbiol 27: 380-395.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)