Symbiotic effect of Bradyrhizobium yuanmingense isolates over Bradyrhizobium japonicum with soybean

DOI: 10.5958/0976-0571.2015.00084.3    | Article Id: LR-3100 | Page : 268-270
Citation :- Symbiotic effect of Bradyrhizobium yuanmingense isolates over Bradyrhizobium japonicum with soybean .Legume Research-An International Journal.2015.(38):268-270
C. Appunu*, Ravinder Kumar, V. Sreenivasa, T. Manjunatha, C. Mahadevaiah, N. Sasirekha, V. R. Prabavathy and Sudha Nair
Address : Division of Molecular Biology, (MSSRF), Taramani Institutional Area, Chennai 600 113, India.


Symbiotic effect of twenty one indigenous Bradyrhizobium yuanmingense strains obtained from nodules of soybean cultivated in India was comparatively evaluated with B. japonicum ASR011 on soybean cv. JS335 for improved nodulation and plant growth performance under greenhouse conditions. Significant variation in symbiotic potential among native B. yuanmingense strains was observed and few of them exhibited significantly superior symbiotic performance i.e. improved the shoot and root dry weights and shoot N content compared to non-inoculated plants and over B. japonicum inoculated plants. The symbiotically efficient B. yuanmingense strains may have applications in the formulation of appropriate inocula to improve soybean crop yield on Indian soils.


B. yuanmingense B. japonicum Nitrogen fixation Soybean.


  1. AOAC. (1990). Official Method of Analysis of the Association of Official Analytical Chemist, 15 Ed, Arlington, VA, pp. 552-553
  2. Appunu, C. and Dhar, B. (2006). Differential symbiotic response of Bradyrhizobium japonicum phage-typed strains with soybean cultivars. J. Microbiol. 44: 363-368
  3. Appunu, C., N’Zoue, A., Laguerre, G. (2008a). Genetic diversity of native bradyrhizobia isolated from soybeans (Glycine max L.) in different agricultural-ecological-climatic regions of India. Appl. Environ. Microbiol. 74: 5991-5996
  4. Appunu, C., Sen, D., Singh, M.K., Dhar, B. (2008b). Variation in symbiotic performance of Bradyrhizobium japonicum strains and soybean cultivars under field conditions. J. Central European Agri. 9: 185-190
  5. Appunu, C., Sasi Rekha, N., Prabavathy, V.R., Nair, S. (2009). A significant proportion of indigenous rhizobia from India associated with soybean [Glycine max (L.)] are distinctly belong to Bradyrhizobium and Ensifer genera. Biol. Fertil. Soils 46: 57-63
  6. Appunu, C., Ganesan, G., Kalita, M., Kaushik, R., Saranya, B., Prabavathy, V.R., Nair, S. (2011). Phylogenetic diversity of rhizobia associated with Horsegram [Macrotyloma uniflorum (Lam.) Verdc.] grown in South India based on glnII, recA and 16S-23S intergenic sequence analyses. Curr. Microbiol. 62: 1230-1238
  7. Hungria, M., Campo, R.J., Chueire, L.M.O., Grange, L., Megias, M. (2001). Symbiotic effectiness of fast-growing rhizobial strains isolated from soybean nodules in Brazil. Biol. Fertil. Soils 33: 387-394
  8. Hungria, M., Campo, R.J., Mendes, I.C. (2003). Benefits of inoculation on common bean (Phaseolus vulgaris) crop with efficient and competitive Rhizobium tropicii strains. Biol. Fertil. Soils 39: 88-93
  9. Meghvanshi, M.K., Prasad, K., Mahna, S.K. (2010). Symbiotic potential, competitiveness and compatibility of indigenous Bradyrhizobium japonicum isolates to three soybean genotypes of two distinct agro-climatic regions of Rajasthan, India. Saudi J. Biol. Sci. 17: 303-310
  10. Melchiorre, M., de Luca, M.J., Anta, G.G., Suarez, P., Lopez, C., Lascano, R., Racca, R.W. (2011). Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants. Biol. Fertil. Soils 47: 81-89
  11. Pule-Meulenberg, F., Gyogluu, C., Naab, J., Dakora, F.D. (2011). Symbiotic N nutrition, bradyrhizobial diversity and photosynthetic functioning of six inoculated promiscuous-nodulating soybean genotypes. J. Plant Physiol. 168: 540-548.
  12. Risal, C.P., Yokoyama, T., Ohkama-Ohtsu, N., Djedidi, S., Sekimoto, H. (2010). Genetic diversity of native soybean bradyrhizobia from different topographical regions along the southern slopes of the Himalayan mountains in Nepal. Syst. Appl. Microbiol. 33:416-425
  13. Sarr, P.S., Yamakawa, T., Fujimoto, S., Saeki, Y., Thao, H.T.B., Myint, A.K. (2009). Phylogenetic diversity and symbiotic effectiveness of root-nodulating bacteria associated with cowpea in the South-West area of Japan. Microbes Environ. 24: 105-112
  14. Vincent J. (1970). A Manual for the Practical Study of the Root Nodule Bacteria. In: Int. Biol. Prog. Handbook, vol. 15. Blackwell Scientific Publications, Oxford, USA.

Global Footprints