IN-SILICO VALIDATION and Comparative analysis OF CANDIDATE GENE ENCODING PROLINE RICH PROTEIN IN LENS CULINARIS

DOI: 10.5958/j.0976-0571.37.2.020    | Article Id: LR-3041 | Page : 133-138
Citation :- IN-SILICO VALIDATION and Comparative analysis OF CANDIDATE GENE ENCODING PROLINE RICH PROTEIN IN LENS CULINARIS.Legume Research-An International Journal.2014.(37):133-138
Jitender Singh*, Juhi Bhardwaj, Pankaj Kumar, Priya Tomar, Anchal Rani, Rosy Rani, Amit Kumar Singh and Anil Sirohi jeets_80@rediffmail.com
Address : College of Biotechnology S.V.P. University of Agric. and Technology, Modipuram-250 110, India

Abstract

In present study a proline rich protein has been identified in lentil EST database using (soyabean) glycine max proline rich protein sequence as query sequence known to be down regulated in response to salinity and drought stress. Sequence similarity search has shown 90 % similarity with chickpea EST sequence Accesion no. GR 398344 and 85% similarity with Lens culinaris EST sequence Accession no. GT 622346. Primer pairs were designed for this sequence and validated on selective Lens culinaris cultivars.  tblastx   result has also shown similarity with the glycine max (Soybean) proline rich protein showing repetitive pattern of amino acids . Repetitive pattern observed was similar to the characteristic pattern present in proline rich protein. Identification of this gene will further help to study the role of proline rich protein in response to various biotic and abiotic stress.

Keywords

EST Lentil Proline rich protein.

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