Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 40 issue 3 (june 2017) : 429-433

26S: Novel reference gene from leaves and roots of common bean for biotic stress expression expression studies based on PCR

Victor Montero-Tavera, María A. Escobedo-Landín, Jorge A. Acosta-Gallegos, José L. Anaya-Lopez, Jorge E. Ruiz-Nieto*
1<p>Campo Experimental Baj&iacute;o, Instituto Nacional de Investigaciones Forestales,&nbsp;Agr&iacute;colas y Pecuarias, Celaya C.P. 38110, Guanajuato, M&eacute;xico.&nbsp;</p>
Cite article:- Montero-Tavera Victor, Escobedo-Land&iacute;n A. Mar&iacute;a, Acosta-Gallegos A. Jorge, Anaya-Lopez L. Jos&eacute;, Ruiz-Nieto* E. Jorge (2017). 26S: Novel reference gene from leaves and roots of common bean for biotic stress expression expression studies based on PCR . Legume Research. 40(3): 429-433. doi: 10.18805/lr.v0iOF.7859.

ABSTRACT

The aim of this study was to evaluate the variation in the expression levels of five traditional housekeeping genes under contrasting conditions of abiotic stress to identify the most stable reference gene. In the evaluation of expression levels by RT-PCR, the gene with less variation among cultivars and treatments was the ribosomal 26S. Regarding to the variation between foliage and root tissues the more stable genes were â-tubulin and 26S, while between phenological stages the best were 26S and the 1-â elongation factor. The evaluation of expression levels by absolute qPCR confirmed that the 26S gene is a stable reference for the study of gene expression based on the PCR technique due to its low coefficient of variation through contrasting abiotic stress conditions.

REFERENCES


  1. Aroca, R. (2006). Exogenous catalase and ascorbate modify the effects of abscisic acid (ABA) on root hydraulic properties in Phaseolus vulgaris L. plants. Plant Growth Regul. 25: 10–17.

  2. Borges, A., Tsai, S.M. and Caldas, D.G.G. (2012). Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Plant Cell Rep. 31: 827–838.

  3. Carvalho, K., de Campos, M., Pereira, L. and Vieira, L. (2010). Reference gene selection for real-time quantitative polymerase chain reaction normalization in “Swingle” citrumelo under drought stress. Anal Biochem. 402: 197–199.

  4. Crosatti, C., Pagani, D., Cattivelli, L., Stanca, A. and Rizza, F. (2008). Effects of growth stage and hardening conditions on the association between frost resistance and the expression of the cold-induced protein COR14b in barley. Environ Exp Bot. 62: 93–100.

  5. Cuellar-Ortiz, S.M., De La Paz Arrieta-Montiel, M., Acosta-Gallegos, J.A. and Covarrubias, A.A. (2008). Relationship between carbohydrate partitioning and drought resistance in common bean. Plant Cell Environ. 31: 1399–1409.

  6. Doyle, J.J. (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

  7. Gopesh, C.S. and George, J.V. (2012). Evaluation of expression stability of candidate references genes among green and yellow pea cultivars (Pisum sativum L.) subjected to abiotic and biotic stress. Am J Plant Sci. 2012.

  8. Gutierrez, L., Mauriat, M., Guénin, S., Pelloux, J., Lefebvre, J.F., Louvet, R., Rusterucci, C., Moritz, T., Guerineau, F., Bellini, C. and Van Wuytswinkel, O. (2008). The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Plant Biotechnol J. 6: 609–618.

  9. Hu, R., Fan, C., Li, H., Zhang, Q. and Fu, Y.F. (2009). Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR. BMC Mol Bio. 10: 93.

  10. Kaymakanova, M. and Stoeva, N. (2008). Physiological reaction of bean plants (Phaseolus vulgaris L.) to salt stress. Gen Appl Plant Physiol Spec. 34: 3–4.

  11. Kizis, D. (2002). Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through the drought-responsive element in an ABA-dependent pathway. Plant J. 30: 679–689.

  12. Lanza, M., Garcia-Ponce, B., Castrillo, G., Catarecha, P., Sauer, M., Rodriguez-Serrano, M., Páez-García, A., Sánchez-Bermejo, E., Mohan, T., Leo-del Puerto, Y.L., Sandalio, L.M., Paz-Ares, J. and Leyva, A. (2012). Role of actin cytoskeleton in brassinosteroid signaling and in its integration with the auxin response in plants. Dev Cell. 22: 1275–1285.

  13. Logemann, J., Schell, J. and Willmitzer, L. (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem. 163:16-20.

  14. Mansour, M. (2000). Nitrogen containing compounds and adaptation of plants to salinity stress. Biol Plant. 43: 491–500.

  15. Meloni, D.A., Oliva, M.A., Martinez, C.A. and Cambraia, J. (2003). Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot. 49: 69–76.

  16. Migocka, M. and Papierniak, A. (2011). Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators. Mol Breed. 28: 343–357.

  17. Mostajeran, A. and Rahimi-Eichi, V. (2009). Effects of drought stress on growth and yield of rice (Oryza sativa L.) cultivars and accumulation of proline and soluble sugars in sheath and blades of their different ages leaves. Agric Env Sci. 5: 264–272.

  18. Nguyen, T. and Sticklen, M. (2012). Genetic transformation of common bean (Phaseolus vulgaris L.) with the gus color marker, the bar herbicide resistance, and the barley (Hordeum vulgare) HVA1 drought tolerance genes. Int J Agron. 2012.

  19. Nicot, N., Hausman, J.F., Hoffmann, L. and Evers, D. (2005). Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot. 56: 2907–2914.

  20. Rosales, M.A., Ocampo, E., Rodríguez-Valentín, R., Olvera-Carrillo, Y., Acosta-Gallegos, J. and Covarrubias, A.A. (2012). Physiological analysis of common bean (Phaseolus vulgaris L.) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiol Biochem. 56: 24–34.

  21. Ruiz-Nieto, J.E., Aguirre-Mancilla, C.L., Acosta-Gallegos, J.A., Raya-Pérez, J.C., Piedra-Ibarra, E., Vázquez-Medrano, J. and Montero-    Tavera, V. (2015). Photosynthesis and chloroplast genes are involved in water-use efficiency in common bean. Plant Physiol Biochem. 86: 166–173.

  22. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular cloning. Cold Spring Harbor Laboratory Press, New York.

  23. Sasvari, Z., Izotova, L., Kinzy, T.G. and Nagy, P.D. (2011). Synergistic roles of eukaryotic translation elongation factors 1Bã and 1A in stimulation of tombusvirus minus-strand synthesis. PLoS Pathog. 7: e1002438.

  24. Thibivilliers, S., Joshi, T., Campbell, K.B., Scheffler, B., Xu, D., Cooper, B., Nguyen, H.T. and Stacey, G. (2009). Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection. BMC Plant Biol. 9: 46.

  25. Thorrez, L., Van Deun, K., Tranchevent, L.C., Van Lommel, L., Engelen, K., Marchal, K., Moreau, Y., Van Mechelen, I. and Schuit, F. (2008). Using ribosomal protein genes as reference: a tale of caution. PloS One. 3: e1854.

  26. Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R. and Leunissen, J. A. (2007). Primer3Plus, an enhanced web interface to Primer3. Nucleic Acid Res. 35: 71-74.

  27. Wahid, A., Gelani, S., Ashraf, M. and Foolad, M.R. (2007). Heat tolerance in plants: an overview. Environ Exp Bot. 61: 199–223.

  28. Yu, Y., Li, Y., Li, L., Lin, J., Zheng, C. and Zhang, L. (2009). Overexpression of PwTUA1, a pollen-specific tubulin gene, increases pollen tube elongation by altering the distribution of á-tubulin and promoting vesicle transport. J Exp Bot. 60: 2737-2749. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)