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volume 38 issue 2 (april 2015) : 246-252,   Doi: 10.5958/0976-0571.2015.00083.1

Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers

M
Madhuri Katkar
S
S.S. Mane*
N
Nivedita Kadam
1Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola -444 104, India.
Cite article:- Katkar Madhuri, Mane* S.S., Kadam Nivedita (2025). Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers. Legume Research. 38(2): 246-252. doi: 10.5958/0976-0571.2015.00083.1.

ABSTRACT

A total of 30 random amplified polymorphic DNA and 16 Inter Sequence Simple Repeat primers were screened against four races of Fusarium oxysporum f sp. ciceri. Twenty three RAPD primers produced scorable bands, averages of 7 bands per primer. The level of polymorphism obtained 96.12%. Off sixteen ISSR primers screened level of polymorphism obtained 92.47%. The similarity co-efficient of RAPD and ISSR banding pattern analysis showed Race- 3 is distinct from other three races of the pathogen. In combined analysis of genetic diversity of the 4 races of the pathogen by using twenty three RAPD and fifteen ISSR primers shown higher level of polymorphism. The UPGMA analysis of RAPD, ISSR and RAPD-ISSR combine analysis grouped Race-1, Race-2, under cluster- A and Race-4 in cluster- B whereas Race- 3 showed distinct out-group type reaction with low similarity index and distinct from other races of Fusarium oxysporum f.sp. ciceri.

REFERENCES

  1. Barve, M.P., Haware, M.P., Sainani, M.N., Ranjekar, P.K. and Gupta, V.S. (2001). Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India. Theor.and Applied Genet. 102: 138-147
  2. Bayraktar, H., Dolar, F.S. and Maden, S. (2008). Use of RAPD and ISSR marker in detection of genetic variation and population, structure among Fusarium oxysporum f. sp. ciceris isolates on Chickpea in. Turkey J. Phytopathol. 156 (3): 146-154
  3. Dahiya, B.S. (2003). Improved cultivars and agricultural practices for chickpea promotion in Punjab and Haryana. In: Proceedings and recommendations: Chickpea production and productivity contracts. ICAR New Delhi 17-24 Honnareddy, N. and Dubey, S.C. (2006) Pathogenic and Molecular characterization of Indian isolates of Fusarium oxysporum f.sp. ciceris causing chickpea wilt. Curr. Sci. 91 (5): 661-666
  4. Haware, M.P. and Nene, Y.L. (1982) Races of Fusarium oxysporum f.sp. ciceris. Pl. Dis. 66: 809-810
  5. Haware, M.P., Jiménez Díaz, R.M., Amin, K.S., Phillips, J.C., and Halila, H. (1990) Integrated management of wilt and root rots of Chickpea. In: Chickpea in the nineties: Proc 2nd Int Workshop Chickpea Improve. ICRISAT Center, Patancheru, India ICRISAT Publication, pp 129–133
  6. Haware, M. P., Nene, Y. L., Pundir, R. P. S. and Rao, J. N.. 1992. Screening of world chickpea germplasm for resistance to Fusarium wilt. Field Crop Res 30: 147-154.
  7. Jimenez-Diaz, R.M., Alcala- Jimenez, A.R., Hervas, A. and Trapero-Casa, J.L. (1993)
  8. Pathogenic variability and host resistance in Fusarium oxysporum f. sp. ciceris. In Proc.3rd Eur. Semin. Fusarium Mycotoxins, Taxonomy, Pathogenicity and Host resistance, Poland 87-94
  9. Jimenez-Gasco, M.M., Perez-Artes, E. and Jimenez-Diaz, R.M. (2001) Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). Euro. J. Pl. Path. 107: 237-248
  10. Jimenez-Gasco,M.M. and Jimenez-Diaz,R.M.(2003).Development of a specific Polymerase Chain Reaction -Based Assay for identification of Fusarium oxysporum f.sp. ciceris and its pathogenic Races 0,lA,5,and 6 Phytopathol 93: 200-209
  11. Jimenez-Gasco, M.M., Milgroom, M.G. and Jimenez-Diaz, R.M. (2004). Stepwise evolution of races in Fusarium oxysporum f.sp. ciceris inferred from fingerprinting with repetitive DNA sequence. APS 94: 228-234
  12. Kelly, A., Alcala-Jimenez, A.R., Bainbridge, B.W., Heale, J.B., Perez-Artes, E. and Jimenez-Diaz, R.M. (1994). Use of genetic fingerprinting and random amplified polymorphic DNA to characterize pathogens of Fusarium oxysporum f. sp. ciceris infecting chickpea. Phytopathol. 4 (11): 1293- 1298.
  13. Murray, M.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight DNA. Nucleic Acid Research 8: 4321-4325
  14. Pawar, K.B., Bendre, N.J., Aher, A.P. and Deshmukh, R.B. (1992). Wilt resistance chickpea lines in Maharashtra state, India International Chickpea News l27: 16-17.
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    volume 38 issue 2 (april 2015) : 246-252,   Doi: 10.5958/0976-0571.2015.00083.1

    Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers

    M
    Madhuri Katkar
    S
    S.S. Mane*
    N
    Nivedita Kadam
    1Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola -444 104, India.
    Cite article:- Katkar Madhuri, Mane* S.S., Kadam Nivedita (2025). Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers. Legume Research. 38(2): 246-252. doi: 10.5958/0976-0571.2015.00083.1.

    ABSTRACT

    A total of 30 random amplified polymorphic DNA and 16 Inter Sequence Simple Repeat primers were screened against four races of Fusarium oxysporum f sp. ciceri. Twenty three RAPD primers produced scorable bands, averages of 7 bands per primer. The level of polymorphism obtained 96.12%. Off sixteen ISSR primers screened level of polymorphism obtained 92.47%. The similarity co-efficient of RAPD and ISSR banding pattern analysis showed Race- 3 is distinct from other three races of the pathogen. In combined analysis of genetic diversity of the 4 races of the pathogen by using twenty three RAPD and fifteen ISSR primers shown higher level of polymorphism. The UPGMA analysis of RAPD, ISSR and RAPD-ISSR combine analysis grouped Race-1, Race-2, under cluster- A and Race-4 in cluster- B whereas Race- 3 showed distinct out-group type reaction with low similarity index and distinct from other races of Fusarium oxysporum f.sp. ciceri.

    REFERENCES

    1. Barve, M.P., Haware, M.P., Sainani, M.N., Ranjekar, P.K. and Gupta, V.S. (2001). Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India. Theor.and Applied Genet. 102: 138-147
    2. Bayraktar, H., Dolar, F.S. and Maden, S. (2008). Use of RAPD and ISSR marker in detection of genetic variation and population, structure among Fusarium oxysporum f. sp. ciceris isolates on Chickpea in. Turkey J. Phytopathol. 156 (3): 146-154
    3. Dahiya, B.S. (2003). Improved cultivars and agricultural practices for chickpea promotion in Punjab and Haryana. In: Proceedings and recommendations: Chickpea production and productivity contracts. ICAR New Delhi 17-24 Honnareddy, N. and Dubey, S.C. (2006) Pathogenic and Molecular characterization of Indian isolates of Fusarium oxysporum f.sp. ciceris causing chickpea wilt. Curr. Sci. 91 (5): 661-666
    4. Haware, M.P. and Nene, Y.L. (1982) Races of Fusarium oxysporum f.sp. ciceris. Pl. Dis. 66: 809-810
    5. Haware, M.P., Jiménez Díaz, R.M., Amin, K.S., Phillips, J.C., and Halila, H. (1990) Integrated management of wilt and root rots of Chickpea. In: Chickpea in the nineties: Proc 2nd Int Workshop Chickpea Improve. ICRISAT Center, Patancheru, India ICRISAT Publication, pp 129–133
    6. Haware, M. P., Nene, Y. L., Pundir, R. P. S. and Rao, J. N.. 1992. Screening of world chickpea germplasm for resistance to Fusarium wilt. Field Crop Res 30: 147-154.
    7. Jimenez-Diaz, R.M., Alcala- Jimenez, A.R., Hervas, A. and Trapero-Casa, J.L. (1993)
    8. Pathogenic variability and host resistance in Fusarium oxysporum f. sp. ciceris. In Proc.3rd Eur. Semin. Fusarium Mycotoxins, Taxonomy, Pathogenicity and Host resistance, Poland 87-94
    9. Jimenez-Gasco, M.M., Perez-Artes, E. and Jimenez-Diaz, R.M. (2001) Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). Euro. J. Pl. Path. 107: 237-248
    10. Jimenez-Gasco,M.M. and Jimenez-Diaz,R.M.(2003).Development of a specific Polymerase Chain Reaction -Based Assay for identification of Fusarium oxysporum f.sp. ciceris and its pathogenic Races 0,lA,5,and 6 Phytopathol 93: 200-209
    11. Jimenez-Gasco, M.M., Milgroom, M.G. and Jimenez-Diaz, R.M. (2004). Stepwise evolution of races in Fusarium oxysporum f.sp. ciceris inferred from fingerprinting with repetitive DNA sequence. APS 94: 228-234
    12. Kelly, A., Alcala-Jimenez, A.R., Bainbridge, B.W., Heale, J.B., Perez-Artes, E. and Jimenez-Diaz, R.M. (1994). Use of genetic fingerprinting and random amplified polymorphic DNA to characterize pathogens of Fusarium oxysporum f. sp. ciceris infecting chickpea. Phytopathol. 4 (11): 1293- 1298.
    13. Murray, M.G. and Thompson, W.F. (1980). Rapid isolation of high molecular weight DNA. Nucleic Acid Research 8: 4321-4325
    14. Pawar, K.B., Bendre, N.J., Aher, A.P. and Deshmukh, R.B. (1992). Wilt resistance chickpea lines in Maharashtra state, India International Chickpea News l27: 16-17.
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