Legume Research

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Legume Research, volume 38 issue 2 (april 2015) : 246-252

Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers

Madhuri Katkar, S.S. Mane*, Nivedita Kadam
1Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola -444 104, India.
Cite article:- Katkar Madhuri, Mane* S.S., Kadam Nivedita (2024). Molecular characterization races of Fusarium oxysporum f.sp. ciceri using RAPD and ISSR markers. Legume Research. 38(2): 246-252. doi: 10.5958/0976-0571.2015.00083.1.

ABSTRACT

A total of 30 random amplified polymorphic DNA and 16 Inter Sequence Simple Repeat primers were screened against four races of Fusarium oxysporum f sp. ciceri. Twenty three RAPD primers produced scorable bands, averages of 7 bands per primer. The level of polymorphism obtained 96.12%. Off sixteen ISSR primers screened level of polymorphism obtained 92.47%. The similarity co-efficient of RAPD and ISSR banding pattern analysis showed Race- 3 is distinct from other three races of the pathogen. In combined analysis of genetic diversity of the 4 races of the pathogen by using twenty three RAPD and fifteen ISSR primers shown higher level of polymorphism. The UPGMA analysis of RAPD, ISSR and RAPD-ISSR combine analysis grouped Race-1, Race-2, under cluster- A and Race-4 in cluster- B whereas Race- 3 showed distinct out-group type reaction with low similarity index and distinct from other races of Fusarium oxysporum f.sp. ciceri.

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