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Screening of antagonistic potential and plant growth promotion activities of Trichoderma spp. and fluorescent Pseudomonas spp. isolates against Sclerotinia sclerotiorum causing stem rot of French bean

DOI: 10.5958/0976-0571.2015.00121.6    | Article Id: LR-2981 | Page : 375-381
Citation :- Screening of antagonistic potential and plant growth promotion activities of Trichoderma spp. and fluorescent Pseudomonas spp. isolates against Sclerotinia sclerotiorum causing stem rot of French bean.Legume Research.2015.(38):375-381
Ruchita Dixit*, Ram Bahadur Singh and Harikesh Bahadur Singh dixitruchita06@gmail.com
Address : Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221 005, India.

Abstract

Sclerotinia sclerotiorum have been a major plant pathogen with wide host range in agriculture. Annually, 60% crop losses due to this pathogen have been reported worldwide. Biological management have been sought to be an alternative method for controlling the disease. Trichoderma and Pseudomonas are well known for their biocontrol and plant growth promotion activities. Isolation was done from nearby and within Varanasi. Among all isolates 20 isolates of Trichoderma and 11 isolates of fluorescent Pseudomonas were selected on the basis of dual culture assay against S. sclerotiorum. They were further characterised on the basis of IAA, siderophore, phosphate solubilization, effect of volatile and crude extract, HCN production, ammonia production, chitinase and ß 1, 3–glucanase enzymatic assay. Among the Trichoderma isolates, maximum plant growth promotion activity and mycolytic enzyme activity was observed by isolate T9. Among fluorescent Pseudomonas isolates the best plant promotion activity as well efficient antagonistic activity was observed in isolate Pf11.

Keywords

Bioagents French bean Sclerotinia sclerotiorum.

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