Legume Research

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Legume Research, volume 38 issue 3 (june 2015) : 279-288

RAPD markers for genetic characterization in mungbean [Vigna radiata (L.) Wilczek]

Swapan K. Tripathy*, Suchinnata S. Sardar, Sasmita Dash, Shovina Pal, Tanuja Acharya, Bhumika Ray Mohapatra, Anath B. Das, Gyana R. Rout
1Sinha Molecular Breeding Laboratory, Department of Plant Breeding & Genetics, College of Agriculture, OUAT, Bhubaneswar-751 003, India.
Cite article:- Tripathy* K. Swapan, Sardar S. Suchinnata, Dash Sasmita, Pal Shovina, Acharya Tanuja, Mohapatra Ray Bhumika, Das B. Anath, Rout R. Gyana (2025). RAPD markers for genetic characterization in mungbean [Vigna radiata (L.) Wilczek]. Legume Research. 38(3): 279-288. doi: 10.5958/0976-0571.2015.00023.5.

ABSTRACT

Information on genetic variability and relatedness among 20 selected high yielding Vigna genotypes were assessed using RAPD profiles. Ten RAPD primers generated 81 amplicons resulting 98.7% polymorphism. 5-12 bands were produced per primer with an average of 8.1 bands and the maximum being resolved using primer OPD 08. All primers revealed 100% polymorphic loci except the primer OPD 15 which produced one monomorphic amplicon (500bp). Jaccard’s similarity coefficient values indicated >80% genomic homology between the T-series mutants of Dhauli (parent variety). T 2-1 had shown two specific bands of amplicon size 750bp and 920bp produced by primer OPD 18. Similarly, a 900bp band amplified by primer OPA-8 was specific to Purusattam local whereas, it was absent in all other local land races. RAPD analysis confirmed that wild accession of mungbean (Vigna radiata var. Sublobata) is more close to mungbean particularly local land races than urdbean. The UPGMA cluster analysis distinguished each single variety resulting single variety cluster at even 0.86 similarity index. Further, the RAPD analysis revealed high divergence of UG 218, LGG460 and TCR 20 from AB 2557, ML 267 and Pusa 172. These genotypes could serve as valuable genetic materials in recombination breeding.

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