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Current IssueEditorial BoardOnline First ArticlesArchive

volume 38 issue 1 (february 2015) : 126-130,   Doi: 10.5958/0976-0571.2015.00021.1

Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]

B
B.C. Ajay*
M
M. Byre Gowda
P
P.S. Prasad
G
G.N. Veerakumar
H
H. Prashanth babu
B
B.N. Gnanesh
S
S.C Venkatesh
K
K.N. Ganapathy
R
R. Abdul Fiyaz
K
K.T. Ramya
1AICRP on Pigeonpea, ZARS, Uniersity of Agricultural Science, GKVK, Bengaluru.
Cite article:- Ajay* B.C., Gowda Byre M., Prasad P.S., Veerakumar G.N., babu Prashanth H., Gnanesh B.N., Venkatesh S.C, Ganapathy K.N., Fiyaz Abdul R., Ramya K.T. (2025). Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.] . Legume Research. 38(1): 126-130. doi: 10.5958/0976-0571.2015.00021.1.

ABSTRACT

An experiment was conducted to identify markers linked to Fusarium wilt disease resistance, Parents namely TTB 7 and ICP 8863 were screened using 151 SSRs markers and 16 AFLP primer combinations. Parental screening revealed five SSR primers and 12 AFLP primer combinations polymorphic between parents. Bulk segregant analysis identified five AFLP primer combinations generating seven markers polymorphic between resistant and susceptible bulks while, none of the SSR markers were polymorphic. This indicates that, these markers are putatively linked to wilt disease. Screening of F2 segregating population of cross TTB 7 x ICP 8863 with these putatively linked markers revealed four markers (E-AAT/M-CTG850, E-TCG/M-CTT650, E-TCG/M-CTA730 and E-TCG/M-CTT230) which segregated in 3:1 mendelian pattern. Simple linear regression performed on these four markers had identified two markers namely E-TCG/M-CTT650 and E-TCG/M-CTA730 linked to disease.

REFERENCES

  1. Arunita, R.S., Singh, J., Chopra, S,K., Balyan, H.S., Gupta, P.K. and Shripad, R.B. (2010) Association of AFLP and SSR markers with agronomic and fibre quality traits in Gossypium hirsutum L. J. Genetics, 89: 155-162
  2. Ben, H.L. (1998) QTL mapping: Single marker analysis. (In) Statistical Genomics- linkage, mapping and QTL analysis. Pp 387-416.
  3. DES (2010) Directorate of Economics and Statistics. Agriculture statistics at glance 2010, India. http://dacnet.nic.in/eands/    latest_2006.htm. Accessed 23 Feb 2011
  4. Eujayl, I., Baum, M., Powell, W., Erskine, W. and Pehu, E. (1998) A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor. Appl. Genet. 97:83–89
  5. Ganapathy, K.N., Byregowda, M., Venkatesh, S.C., Ramachandra, R., Gnanesh, B.N. and Girish, G. (2009) Identification of AFLP markers linked to sterility mosaic disease in pigeonpea Cajanus cajan (L.) Millsp. Int. J. Integ. Biol. 7: 145-149.
  6. Gnanesh, B.N. (2010) Generation of expressed sequenced tags (ESTs) and marker development for sterility mosaic disease (SMD) resistance in pigeonpea [Cajanus cajan (L.) Millsp]. Ph.D. (Agri.) Thesis, UAS, Bangalore .
  7. Hamwieh, A., Udupa, S.M, Choumane, W., Sarker, A., Dreyer, F., Jung, C. and Baum, M. (2005) A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance. Theor. Appl. Genet. 110: 669–677
  8. Keim, P., Schupp, J.M., Travis, S.E., Clayton, K., Zhu, T., Shi, L., Ferreira A. and Webb, D.M. (1997) A high density soybean genetic map based on AFLP markers, Crop. Sci. 37: 537–543.
  9. Kotresh, H., Fakrudin, B., Punnuri, S.M., Rajkumar, B.K., Thudi, M., Paramesh, H., Lohithswa, H. and Kuruvinashetti, M.S. (2006) Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.) Euphytica 149: 113 - 120.
  10. Liu, S., Cantrell, R.G., McCarty, J.C. and Stewart, J.M. (2000) Simple sequence repeat-based assessment of genetic diversity in cotton race stock accessions. Crop Sci. 40: 1459–1469.
  11. Lu, Y.H., Melero-Vera, J.M., Garcia-Tejada, Blanchard, P. (2000) Development of SCAR markers linked to the gene Or5 conferring resistance to broomrape (Orobanche cumana Wallr.) in sunflower. Theor. Appl. Genet. 100: 625-632.
  12. Michelmore, R.W., Paran, I. and Kesseli, R.V. (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. U.S.A 88: 9828–9832.
  13. Odeny, D.A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J. and Gebhardt, C., (2007) Development characterization and utilization of microsatellite markers in pigeonpea [Cajanus cajan (L.) Millsp.]. Plant Breed. 126: 130-137.
  14. Paran, I. and Michelmore, R.W. (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85: 985-993.
  15. Pearson, K. (1922) On the chi-square test of goodness of fit. Biometrika, 14:186-418.
  16. Saifulla, M., Byregowda, M., Mahesh, M. and Nagesha, G.K. (2005) Screening of pigeonpea promising genotypes against wilt disease caused by Fusarium udum over five years. In: Nat.Semin.Emer.trends in Pl. Pathol. and their social relevance. p35, Annamalai Nagar. March 7-8th, 2005.
  17. Saxena, K.B., Kumar, R.V. and Rao, P.V. (2002) Pigeonpea nutrition and its improvement. In: Quality improvement in crops. The Food Products Press. Crop Sci. 42: 227-260.
  18. Shen, X.L., Guo, W.Z., Zhu, X.F., Yuan, Y.L., Yu, J.Z., Kohel, R.J. and Zhang, T.Z. (2005) Molecular mapping of QTLs for fibre qualities in three diverse lines in upland cotton using SSR markers. Mol. Breed. 15, 169-181.
  19. Vos P, Hogers R, Bleekers M, Rejians M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kiuper M, Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.
  20. William, H., Singh, R., Huerta–Espino, J. and Rosewarne, G. (2007) Characterization of Genes for Durable Resistance to Leaf Rust and Yellow Rust in Cimmyt Spring Wheats. Developments in Plant Breeding, 12: 65-70.
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    volume 38 issue 1 (february 2015) : 126-130,   Doi: 10.5958/0976-0571.2015.00021.1

    Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]

    B
    B.C. Ajay*
    M
    M. Byre Gowda
    P
    P.S. Prasad
    G
    G.N. Veerakumar
    H
    H. Prashanth babu
    B
    B.N. Gnanesh
    S
    S.C Venkatesh
    K
    K.N. Ganapathy
    R
    R. Abdul Fiyaz
    K
    K.T. Ramya
    1AICRP on Pigeonpea, ZARS, Uniersity of Agricultural Science, GKVK, Bengaluru.
    Cite article:- Ajay* B.C., Gowda Byre M., Prasad P.S., Veerakumar G.N., babu Prashanth H., Gnanesh B.N., Venkatesh S.C, Ganapathy K.N., Fiyaz Abdul R., Ramya K.T. (2025). Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.] . Legume Research. 38(1): 126-130. doi: 10.5958/0976-0571.2015.00021.1.

    ABSTRACT

    An experiment was conducted to identify markers linked to Fusarium wilt disease resistance, Parents namely TTB 7 and ICP 8863 were screened using 151 SSRs markers and 16 AFLP primer combinations. Parental screening revealed five SSR primers and 12 AFLP primer combinations polymorphic between parents. Bulk segregant analysis identified five AFLP primer combinations generating seven markers polymorphic between resistant and susceptible bulks while, none of the SSR markers were polymorphic. This indicates that, these markers are putatively linked to wilt disease. Screening of F2 segregating population of cross TTB 7 x ICP 8863 with these putatively linked markers revealed four markers (E-AAT/M-CTG850, E-TCG/M-CTT650, E-TCG/M-CTA730 and E-TCG/M-CTT230) which segregated in 3:1 mendelian pattern. Simple linear regression performed on these four markers had identified two markers namely E-TCG/M-CTT650 and E-TCG/M-CTA730 linked to disease.

    REFERENCES

    1. Arunita, R.S., Singh, J., Chopra, S,K., Balyan, H.S., Gupta, P.K. and Shripad, R.B. (2010) Association of AFLP and SSR markers with agronomic and fibre quality traits in Gossypium hirsutum L. J. Genetics, 89: 155-162
    2. Ben, H.L. (1998) QTL mapping: Single marker analysis. (In) Statistical Genomics- linkage, mapping and QTL analysis. Pp 387-416.
    3. DES (2010) Directorate of Economics and Statistics. Agriculture statistics at glance 2010, India. http://dacnet.nic.in/eands/    latest_2006.htm. Accessed 23 Feb 2011
    4. Eujayl, I., Baum, M., Powell, W., Erskine, W. and Pehu, E. (1998) A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor. Appl. Genet. 97:83–89
    5. Ganapathy, K.N., Byregowda, M., Venkatesh, S.C., Ramachandra, R., Gnanesh, B.N. and Girish, G. (2009) Identification of AFLP markers linked to sterility mosaic disease in pigeonpea Cajanus cajan (L.) Millsp. Int. J. Integ. Biol. 7: 145-149.
    6. Gnanesh, B.N. (2010) Generation of expressed sequenced tags (ESTs) and marker development for sterility mosaic disease (SMD) resistance in pigeonpea [Cajanus cajan (L.) Millsp]. Ph.D. (Agri.) Thesis, UAS, Bangalore .
    7. Hamwieh, A., Udupa, S.M, Choumane, W., Sarker, A., Dreyer, F., Jung, C. and Baum, M. (2005) A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of fusarium vascular wilt resistance. Theor. Appl. Genet. 110: 669–677
    8. Keim, P., Schupp, J.M., Travis, S.E., Clayton, K., Zhu, T., Shi, L., Ferreira A. and Webb, D.M. (1997) A high density soybean genetic map based on AFLP markers, Crop. Sci. 37: 537–543.
    9. Kotresh, H., Fakrudin, B., Punnuri, S.M., Rajkumar, B.K., Thudi, M., Paramesh, H., Lohithswa, H. and Kuruvinashetti, M.S. (2006) Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.) Euphytica 149: 113 - 120.
    10. Liu, S., Cantrell, R.G., McCarty, J.C. and Stewart, J.M. (2000) Simple sequence repeat-based assessment of genetic diversity in cotton race stock accessions. Crop Sci. 40: 1459–1469.
    11. Lu, Y.H., Melero-Vera, J.M., Garcia-Tejada, Blanchard, P. (2000) Development of SCAR markers linked to the gene Or5 conferring resistance to broomrape (Orobanche cumana Wallr.) in sunflower. Theor. Appl. Genet. 100: 625-632.
    12. Michelmore, R.W., Paran, I. and Kesseli, R.V. (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. U.S.A 88: 9828–9832.
    13. Odeny, D.A., Jayashree, B., Ferguson, M., Hoisington, D., Crouch, J. and Gebhardt, C., (2007) Development characterization and utilization of microsatellite markers in pigeonpea [Cajanus cajan (L.) Millsp.]. Plant Breed. 126: 130-137.
    14. Paran, I. and Michelmore, R.W. (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85: 985-993.
    15. Pearson, K. (1922) On the chi-square test of goodness of fit. Biometrika, 14:186-418.
    16. Saifulla, M., Byregowda, M., Mahesh, M. and Nagesha, G.K. (2005) Screening of pigeonpea promising genotypes against wilt disease caused by Fusarium udum over five years. In: Nat.Semin.Emer.trends in Pl. Pathol. and their social relevance. p35, Annamalai Nagar. March 7-8th, 2005.
    17. Saxena, K.B., Kumar, R.V. and Rao, P.V. (2002) Pigeonpea nutrition and its improvement. In: Quality improvement in crops. The Food Products Press. Crop Sci. 42: 227-260.
    18. Shen, X.L., Guo, W.Z., Zhu, X.F., Yuan, Y.L., Yu, J.Z., Kohel, R.J. and Zhang, T.Z. (2005) Molecular mapping of QTLs for fibre qualities in three diverse lines in upland cotton using SSR markers. Mol. Breed. 15, 169-181.
    19. Vos P, Hogers R, Bleekers M, Rejians M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kiuper M, Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414.
    20. William, H., Singh, R., Huerta–Espino, J. and Rosewarne, G. (2007) Characterization of Genes for Durable Resistance to Leaf Rust and Yellow Rust in Cimmyt Spring Wheats. Developments in Plant Breeding, 12: 65-70.
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