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INFLUENCE OF GROWTH REGULATORS ON CALLUS INDUCTION AND PLANT REGENERATION IN ARACHIS PROSTRATA (L.)

DOI: 10.5958/j.0976-0571.37.3.042    | Article Id: LR-2907 | Page : 281-286
Citation :- INFLUENCE OF GROWTH REGULATORS ON CALLUS INDUCTION AND PLANT REGENERATION IN ARACHIS PROSTRATA (L.) .Legume Research.2014.(37):281-286
S.K. Bera*, A.L. Singh and M.V. Gedia berask67@yahoo.co.in
Address : Directorate of Groundnut Research, Ivnagar Road, Junagadh- 362 001, India

Abstract

Response due to three auxins (NAA, 2,4-D and Picloram) and two cytokinins (BAP and TDZ) was tested for rapid and high frequency in vitro leaf callogenesis in Arachis prostrata. MS media containing 30g/l sucrose and 6g/l agar were supplemented with 10 concentrations of auxins alone and in combination with three concentrations of cytokinin. Sole picloram regardless of concentration, induced significantly higher callogenesis in comparison to NAA and 2,4-D. The response due to NAA, 2,4-D and PIC was more when supplimented with either BAP or TDZ. Picloram in combination of either BAP or TDZ was more effective in leaf callogenesis than NAA and 2,4-D. PIC regardless of concentration with 1.5mg/l BAP produced highest mean callogenesis ranging 99.7-100.0%. Plants regenerated from callus were subcultured in MS media supplemented with NAA+TDZ and 2,4-D +TDZ after 30 days. No regeneration was observed in media supplemented with PIC+TDZ. Maximum regeneration recorded in media supplemented with 3.0mg NAA+0.3mg TDZ per liter. Regeneration in low concentration (0.3mg/L) of TDZ along with NAA and 2,4-D indicates highly inductive effect of TDZ.

Keywords

Growth hormones Leaf callogenesis Wild groundnut.

References

  1. Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrition requirements of suspension cultures of soybean root cells. Cell Res., 50 : 151-158.
  2. Lu, S., Peng, X., Guo, Z., Wang, Z., Wang, C., Pang, C., Fan, Z. and Wang, J. (2007). In vitro selection of salinity tolerant variants triploid bermudagrass (Cynodon transvaalensis x C. dactylon) and their physiological responses to salt and drought stress. Plant Cell Rep. 26 : 1413-1420.
  3. Luan, Y.S., Zhang, J., Xiao, R.G. and Jia, A.L. (2007). Mutation induced by EMS in vitro screening for salt tolerance and plant regenration of sweet potato (Ipomoea batatas L.). Plant Cell, Tissue and Organ Culture 88 : 77-81.
  4. Mansoori, T.A.A., Alaa El-Deen, M.N. and Caligari, P.D.S. (2007). Evaluation of invitro screening techniques for salt tolerance in date palm ISHS. Acta Horticulturae, 736: III international date palm conference.
  5. Melchers, G. and Bergman, L. (1959). Untersuchungen an kulturen von haploiden Gewben von Antirrhinum majus. Ber Dtsch. Bot Ges. 78 : 21-29.
  6. Morginski, E., Rey, H.Y., Gonzalez, A.M. and Morginski, L.A. (2004). Thidiazuran promotes In vitro Regeneration of Arachis correntina(Leguminosae) via organogenesis. J. Plant Growth Regul. 23 : 129-134.
  7. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15 : 473-497.
  8. Queiros, F., Fidalgo, F., Santos, I. and Salema, R. (2007) In vitro selection of salt tolerant cell lines in solanum tuberosum. Biologia Plantarum 51 : 728-734.
  9. Rey, H.Y., Scocchi, A.M., Gonzalez, A.M. and Morginski, L.A. (2000). Plant regeneration in Arachis pintoi through leaf culture. Plant Cell Rep. 19: 856-862.
  10. Sarin, N.B., Gupta, A., Prakash, S. and Biswas, B. (1991). Isolation and characterization of salt tolerant line of Arachis hypogaea using in vitro culture. Acta Horticulturae 289: 219-222.
  11. Singh, R.K. and Chaudhary, B.D. (1985). Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publishers, N. Delhi, pp. 50-52.
  12. Vidoz, M.L., Klusacek, P., Rey, H.Y. and Morginski, L.A. (2006). In vitro plant regeneration of Arachis correntina through somatic embryogenesis and organogenesis. Plant Cell, Tissue and Organ Culture 86: 111-116.
  13. Vidoz, M.L., Rey, H.Y., Gonzalez, A.M. and Morginski, L.A. (2004).Somatic embryogenesis and plant regeneratio through leaf culture in Arachis glabrata (Leguminosae). Acta Physiol. Plant 26: 59-66.
  14. Yokoi, S., Roy, A.B. and Paul, M.H. (2002). Salt tolerance of plants, In: JIRCAS Working Report. Center for environmental Stress Physiology, Purdue University, West Lafayette, USA pp.25-33.
  15. Yucesan, B., Turker, A.U. and Gurel, E. (2007). TDZ induced high frequency regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L). Plant Cell, Tissue and Organ Culture 91:243-250.

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