Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Legume Research, volume 40 issue 1 (february 2017) : 55-62

Effects of drought stress on physiological and biochemical changes in Phaseolus vulgaris L.

Sebnem Kusvuran1, H. Yildiz Dasgan2
1<p>Cankiri Karatekin University Kizilirmak Vocational High School,&nbsp;18100, Cankiri, Turkey.</p>
Cite article:- Kusvuran1 Sebnem, Dasgan2 Yildiz H. (2017). Effects of drought stress on physiological and biochemicalchanges in Phaseolus vulgaris L. . Legume Research. 40(1): 55-62. doi: 10.18805/lr.v0i0.7025.

ABSTRACT

The present study investigated different levels (S1: 100% of field capacity- Control S2: 50% of field capacity-moderate stress; S3: 0% of field capacity-severe stress) of drought stress on oxidative damages and variations in antioxidants in the two bean varieties Bn-16 (drought-sensitive), Bn-150 (drought-tolerant) to elucidate the antioxidative protective mechanism governing differential drought tolerance. The shoot fresh weight, shoot height, leaf number and area, RWC were reduced with different level of drought stress. However this reduction clearly occurred in Bn-16 (sensitive).  Antioxidative enzyme activities, such as superoxide dismutase, catalase, ascorbate peroxidase and glutation reductase, had a greater increase in tolerant genotypes (Bn-150) than in sensitive ones (Bn-16). The level of lipid peroxidation was measured by estimating malondialdehyde content. Lipid peroxidation increased with increasing drought conditions in all genotypes, although Bn-150 was the least affected when compared with the other genotype. Total phenolic and flavonoid content increased in bean genotypes under S2 and S3 conditions. The highest total phenolic and flavonoid contents were attained in Bn-150 subjected to S3 treatment. These results indicated that an antioxidant defence system, osmolytes (such as proline), and secondary metabolites play important roles in common bean (Phaseolus vulgaris L) during drought stress and recovery.

REFERENCES


  1. Anjum SA, Farooq M, Xie X, Liu XJ and Ijaz MF. (2012). Antioxidant defense system and proline accumulation enables hot pepper to perform better under drought. Science Horticulture, 140:66-73.

  2. Ashraf M and Foolad M. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59: 206-216.

  3. Bates LS, Waldren RP and Teare ID. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39: 205-207.

  4. Cakmak I , Marschner H. (1992). Magnesium defficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98: 1222-1226.

  5. Dasgan HY, Kusvuran S, Aydoner G, Akyol M, Akhoundnejad Y, Küçükkömürcü S, Kaya E, Sari N and Abak K. (2010). Screening and saving of local vegetables for their resistance to drought and salinity. MDG- F Project (2009-    2010).

  6. Dawood MG, Taie HA, Nassar A, Abdelhamid MT and Schmidhalter U. (2014). The changes induced in the physiological, biochemical and anatomical characteristics of Vicia faba by the exogenous application of proline under seawater stress. South African Journal of Botany, 93: 54-63.

  7. Dolatabadian A, Sanavy SAMM and Chashmi NA. (2008). The effects of foliar application of ascorbic acid (vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science, 194: 206-213.

  8. Dubey RS. (1999). Protein synthesis by plants under stressful conditions. Handbook of Plant and Crop Stress, 2: 365-397.

  9. Emam Y, Shekoofa A, Salehi F and Jalali AH. (2010). Water stress effects on two common bean cultivars with contrasting growth habits. Am–Eur J Agric Environ Science, 9: 495-499.

  10. Gua Z, Ou W, Lu S and Zhong Q. (2006). Differential Responses of Antioxidative System to Chilling and Drought in Four Rice Cultivars Differing in Sensitivity. Plant Physiology and Biochemistry. 44: 828-836.

  11. Heath RL and Packer L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.

  12. Hodges MD, Andrews CJ, Johnson DA and Hamilton RI. (1997). Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines. Journal Experimental Botany, 48: 1105-1113.

  13. Jia X, Sun C, Li G, Li G and Chen G. (2015). Effects of progressive drought stress on the physiology, antioxidative enzymes and secondary metabolites of Radix Astragali. Acta Physiologiae Plantarum, 37: 1-14.

  14. Karanlik S.(2001). Resistance to salinity in different wheat genotypes and physiological mechanisms involved in salt resistance. Ph.D. Thesis. Institute of Natural and Applied Sciences, University of Cukurova, Adana, Turkey. 122 pp. (In Turkish).

  15. Kaymakanova M and Stoeva N. (2008). Physiological reaction of bean plants (Phaseolus vulg. L.) to salt stress. Genetic Apply Plant Physiology, Special, 34: 3-4.

  16. Kusvuran S., Dasgan YH and Abak K. (2011). Responses of different melon genotypes to drought stress. Yüzüncü Yil University Journal of Agricultural Sciences. 21:209-219.

  17. Kusvuran A. (2015). The effects of salt stress on the germination and antioxidative enzyme activityof Hungarian vetch (Vicia pannonica Crantz.) varieties. Legume Research, 38: 51-59.

  18. Kusvuran S, Kiran S and Ellialtioglu ÞÞ. (2016). Antioxidant Enzyme Activities and Abiotic Stress Tolerance Relationship in Vegetable Crops. In book: Abiotic and Biotic Stress in Plants- Recent Advances and Future Perspectives, Chapter: Chapter 21, Publisher: Intech, Editors: Arun K. Shanker, Chitra Shanker, pp.481-506.

  19. Lei Y, Yin C and Li C. (2006). Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii. Physiologia Plantarum, 127: 182-191.

  20. Li Z, Peng Y and Ma X. (2013). Different response on drought tolerance and post-drought recovery between the small-    leafed and the large-leafed white clover (Trifolium repens L.) associated with antioxidative enzyme protection and lignin metabolism. Acta physiologiae Plantarum, 35: 213-222.

  21. Mafakheri A, Siosemardeh A, Bahramnejad B, Struik PC and Sohrabi Y. (2010). Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science, 4: 580.

  22. Maggio A, Bressan R, Ruggiera C, Xiong L, Grillo S. (2003). Salt tolerance: Placing advences in molecular genetics into a physiological and agronimic context. Abiotic Stresses in Plants. [Ed: L. Toppi and B. Pawlik- Skowronska]. 53-71.

  23. Mansori M, Chernane H, Latique S, Benaliat A, Hsissou D and El Kaoua M. (2015). Seaweed extract effect on water deficit and antioxidative mechanisms in bean plants (Phaseolus vulgaris L.). Journal of Applied Phycology, 27: 1689-1698.

  24. Medina-Juárez LA, Molina-Quijada DM, Del Toro-Sánchez CL, González-Aguilar GA and Gámez-Meza N. (2012). Antioxidant activity of peppers (Capsicum annuum L.) extracts and characterization of their phenolic constituents. Interciencia, 37: 588-593.

  25. Molina-Quijada DMA, Medina-Juárez LA, González-Aguilar GA, Robles-Sánchez RM and Gámez-Meza N. (2010). Phenolic compounds and antioxidant activity of table grape (Vitis vinifera L.) skin from northwest Mexico. Ciencia y Tecnologia Alimentaria, 8: 57–63.

  26. Morsy MR, Jouve L, Hausman JF, Hoffmann L and Stewart JM. (2007). Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. Journal Plant Physiology, 164:157-167.

  27. Pugnaire FI, Serrano LUIS and Pardos JOSE. (1999). Constraints by water stress on plant growth. Handbook of Plant and Crop Stress, 2: 271-283.

  28. Rao PB, Kaur A and Tewari A. (2008). Drought resistance in seedlings of five important tree species in Tarai region of Uttarakhand. Tropical Ecology, 49: 43.

  29. Rosales MA, Ocampo E, Rodríguez-Valentín R, Olvera-Carrillo Y, Acosta-Gallegos J and Covarrubias AA. (2012). Physiological analysis of common bean (Phaseolus vulgaris L.) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiology and Biochemistry, 56: 24-34.

  30. Scandalios JG. (1993). Oxygen stress and superoxide dismutases. Plant Physiology, 101: 7–12.

  31. Sen A. (2012). Oxidative stress studies in plant tissue culture. Antioxidant Enzyme, 3: 59-88.

  32. Shalata A, Mittova V, Guy M and Tal M. (2001). Response of cultivated tomato and its wild salt tolerant relative Lycopersiconpennelli to salt dependent oxidative stress: The root antioxidative system. Physiologia Plantarum. 112: 487–494.

  33. Sharma P, Jha AB, Dubey RS and Pessarakli M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 26 pp.

  34. Shinozaki K, Yamaguchi K, Liu Q, Kasuga M, Ichimura K, Mizoguchi T, Urao T, Miyata S, Nakashima K, Shinwari ZK, Abe H, Sakuma Y, Ito T and Seki M. (1999). Molecular responses to drought stress in plant regulation of gene expression and signal transduction. Plant Responses to Environmental Stress, Chapter 17: 133-143.

  35. Singleton L, Orthofer R, Lamuela-Raventos M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299: 152-178.

  36. Siringam K, Juntawong N, Cha-Um S and Kirdmanee C. (2011). Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under isoosmotic conditions. African Journal of Biotechnology, 10: 1340–1346.

  37. Slabbert MM and Krüger GHJ. (2014). Antioxidant enzyme activity, proline accumulation, leaf area and cell membrane stability in water stressed Amaranthus leaves. South African Journal of Botany, 95: 123-128.

  38. Terzi R, Saglam A, Kutlu N, Nar H and Kadioglu A. (2010). Impact of soil drought stress on photochemical efficiency of photosystem II and antioxidant enzyme activities of Phaseolus vulgaris cultivars. Turkish Journal of Botany, 34: 1-10.

  39. Wang CY. (1995). Temperature preconditioning affects glutathione content and glutathione reductase activity in chilled zucchini squash. Journal Plant Physiology, 145:148-152.

  40. Yuan G, Wang X, Guo R and Wang Q. (2010). Effect of salt stress on phenolic compounds, glucosinolates, myrosinase and antioxidant activity in radish sprouts. Food Chemistry, 121: 1014-1019.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)