SUITABILITY OF SSR MARKER FOR ESTABLISHING DISTINCTIVENESS IN FRENCH BEAN VARIETIES (PHASEOLUS VULGARIS L.)

ABSTRACT

Eleven French bean varieties (Phaseolous vulgaris L.) were studied for SSR molecular marker utilizing ten SSR primers. It revealed 32 alleles in 11 varieties with an average of 3.2 alleles / locus. The number of alleles per locus varied from 1 to 5. The size of allele amplified varied from 50 to 500bp. Cluster analysis was done using pairwise Jaccard’s similarity co-efficient with similarity value ranges from 37% to 94%. The PIC value in the present study ranged from 0 to 0.79, with an average of 0.37 per primer pair. Specific loci were obtained in four local varieties namely Chakrata Local-2 PGR FB 2-07, Bhura Lal Swant, Lal Thumri Swant and Chitkabra Lal Jhulu Swant by 10 SSR primers which could unambiguously discriminate these varieties. When all the morphological and SSR molecular marker were considered then eight varieties could be distinguished. Study showed that for establishing distinctiveness in varieties, besides morphological characters some other DNA based markers might also be considered.

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SUITABILITY OF SSR MARKER FOR ESTABLISHING DISTINCTIVENESS IN FRENCH BEAN VARIETIES (PHASEOLUS VULGARIS L.)

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