Legume Research

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Research Article

Legume Research, volume 40 issue 2 (april 2017) : 264-270

The pharmacologically active components of Oxytropis falcata bunge reduce ischemic-reperfusion injury in the rat heart

Dejun Zhang*, Ruofei Jiang, Eun-Kyung Hong, Guosheng Tan, Dianxiang Lu, Yongping Li, Wangtao Yuan
1<p>State Key Laboratory of Plateau Ecology and Agriculture,&nbsp;Qinghai University, Xining 810016, P.R.China.</p>
Cite article:- Zhang* Dejun, Jiang Ruofei, Hong Eun-Kyung, Tan Guosheng, Lu Dianxiang, Li Yongping, Yuan Wangtao (2017). The pharmacologically active components of Oxytropis falcata bungereduce ischemic-reperfusion injury in the rat heart . Legume Research. 40(2): 264-270. doi: 10.18805/lr.v0i0.7592.

ABSTRACT

Oxytropis falcata is used as the main material to prepare various Tibetan traditional medicine, which its activity was reported that anti-inflammation and analgesia. Flavonoids is the chemical basis for the anti-inflammatory and anti-oxidant efficacy. The influence of total flavonoids of Tibetan herb O. falcata on myocardial ischemic-reperfusion injury was investigated using ethanol, chloroform, and ethyl acetate extracts. Rats were pretreated with different concentrations of the extract for 7 days. The model of myocardial ischemic-reperfusion injury was established by ligating the left anterior descending (LAD) artery in the heart. Following reperfusion for 40 minutes, the enzyme activity of creatine kinase (CK), lactic acid dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), and the concentration of malondialdehyde (MDA) in the rat serum were measured. The ultrastructural pathological change was observed under transmission electron microscopy (TEM). The serum levels of CK, LDH, and MDA were reduced, and the activity of SOD and GSH-Px were increased significantly, by chloroform and ethyl acetate extracts (P<0.01, P<0.05). The ultrastructural pathological change in myocardial ischemic-reperfusion injury was alleviated by both extracts. The major components in chloroform extract were 7-hydroxy-flavonone and 2',4'-dihydroxy chalcone. 7-Hydroxy-flavanon naringenine naringetol and 2',4'-dihydroxy chalcone were the major components in ethyl acetate extract. Total flavonoids of Tibetan herb O. falcata might alleviate myocardial ischemic-reperfusion injury.

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  26. Huo X. H., Zhao B. Y., Wang J. J., Wan X. P., and Long A. L. (2008). Preliminary chemical test of O. falcata Bunge and alkaloids analysis by TLC. Acta Agriculture Boreali-occidentalis Sinica 17: 24 - 28, 32.

  27. Jiang H. F. (2007). Levosimendan on isolated rat myocardial ischemia-reperfusion injury in rats. Graduate School of Tianjin Medical University. pp.5.

  28. Li J., Cao M. M. and Gao Y. Y. (2012). Salvia compatibility with Ukrainian gland Hypericum animal models of myocardial ischemia impact study. Journal of Traditional Chinese Medicine 40: 17 - 19.

  29. Liu B. (1997). China Oxytropismedicinal plants and modern research. Chinese Wild Plant Resources 16: 15 - 18.

  30. Min Qing, Bai Yuting, Yu Wei, Wu J.L., and Liu Y.T. (2011). Protective Effect and the Mechanism of Crataegi Folium Flavonoids on Myocardial Ischemia in Rats. Chinese Journal of Modern Applied Pharmacy 28: 95 - 99.

  31. Peter H. Raven, Libing Zhang, Ihsan A. Al-Shehbaz, Nicholas J. Turland, Zhu Guanghua. et al. (2010). Flora of China, 10:453.

  32. Philip E., Shaheen., Walters., Paul E., Jennifer K., Eric W.Q., Ronald M., Bukowski. (2005). Phasea! study of the efficacy and safety of oral GD0039 in patients with locally advanced or metastatic renal cell arcinoma. Investigational New Drugs 23: 577 - 581.

  33. Qin J., Li H. L., Zhang R. P., and Zheng C.L. (2006). Cardio-Protection of SPM(Salvia przewalskii Maxim var mandarinorumstib.) during acute myocardial ischemia/reperfusion injury in rats in vivo. Nat. Prod. Res. Dev.18: 92 - 94.

  34. Tan H. L., Sun Y., Tan F. Z., Zhou Y.W., and Huang R.B. (2009). Persimmon leaf flavonoids on myocardial ischemia-    reperfusion injury in rats. Pharmacology and Clinics 25: 27 - 29. 

  35. Wang C. J., Gao M. T., Shi X. Y., Wu Y.J., and Li W.G. (2003). Procyanidin on myocardial ischemia-reperfusion injury in rats. Chinese Journal of Clinical Pharmacology and Therapeutics 8: 173 - 175. 

  36. Wang D., Yang H., Yang G. M., Tong L., and Cai B.C. (2010). Volatile compositions from A Tibetan Medicine: O. falcata Bunge. Nat. Prod. Res. Dev. 22: 614 - 619.

  37. Wang Dong. (2010). Tibetan medicine O. falcata inflammatory flavonoids and antioxidant activity of its determination. College of Pharmacy in Nanjing University of Traditional Chinese Medicine. 56 - 58.

  38. Wang S. S., Zhang X. J., Que S., and Zhang Q.Y. (2012). 3-Hydroxy-3-methylglutaryl flavonolglycosides from O. falcata. J. Nat. Prod. 75: 1 359 - 1 364.

  39. Xu L. P. (2002). Xionggui Capsule on protective effect of ischemic myocardium in mice. Pharmacology and Clinics 18: 39.

  40. Yan Q., Bai Y. T., Zhang Z., and Shi L.Y. (2007). Hawthorn leaves flavonoids on myocardial ischemia-reperfusion injury electrocardiogram, nitric oxide and malondialdehyde change impacts. Chinese Journal of Clinical Pharmacology and Therapeutics 12: 800 - 803.

  41. Yang H. (2010). Anti-inflammatory activity and chemical constituents of Tibetan medicine O. falcata.Nanjing University of Traditional Chinese Medicine. pp.45-55.

  42. Yoshiki N. (1997). The role of nitric oxide in cardiol-reperfusion injury. J.Pn.Cire. 61.

  43. Zhang X. D., Jiang W. Z., Jiao Y., and Huang R.B. (2008). Longyanshen flavonoids myocardial ischemia-reperfusion induced lipid peroxidation injury research. Shizhen guoyi guoyao. 19: 607 - 609.

  44. Zhang X. J., Li L. Y., Wang S. S., Que S., Cheng W., Liang H., Zhao Y. Y., and Zhang Q.Y.(2014). Flavonoids from Tibetan medicine O. falcata Bunge. J. Chin. Pharm. Sci. 23: 99 - 105.

  45. Zhao Y., Tong L., Chen S. L., Yuan D.P., Zhou T., and Li S.,(2009). The Effects of O. falcata Bunge Ethanol Extract on the proliferation and apoptosis of the human hepatoma cell. Progress in Veterinary Medicine 30: 15 - 18.  
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