Legume Research

  • Chief EditorJ. S. Sandhu

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Legume Research, volume 39 issue 6 (december 2016) : 914-920

Anti-hypoxia effects of the ethanol extract of Oxytropis ochrocephala 

Dejun Zhang1, 2*, Jiang Lei2, Eun-Kyung Hong3, Dianxiang Lu2, Wangtao Yuan2, Zhanting Yang2, Chen Ming2
1<p>State Key Laboratory of Plateau Ecology and Agriculture,&nbsp;Qinghai University, Xining 810016, P.R.China.</p>
Cite article:- Zhang1 Dejun, 2*, Lei2 Jiang, Hong3 Eun-Kyung, Lu2 Dianxiang, Yuan2 Wangtao, Yang2 Zhanting, Ming2 Chen (2016). Anti-hypoxia effects of the ethanol extract of Oxytropis ochrocephala . Legume Research. 39(6): 914-920. doi: 10.18805/lr.v0iOF.3544.

Oxytropis ochrocephala is one of the most extensively used herbs in traditional Tibetan folk medicine to clear heat through detumescence, strengthen the body, and improve immune system. However, research studies examining its medicinal value are limited. By conducting experiments using animal models (mice) in airtight space at ambient and reduced pressure, as well as sodium nitrite exposure, the anti-hypoxia effects of the ethanol extract of O. ochrocephala (EEOO) were investigated in the present study.   

EEOO was administered to three groups of mice for 7 days; 1.5 g/kg for the high-dosage group (EHD), 1.0 g/kg for the medium-dosage group (EMD) and 0.5 g/kg for the low-dosage group (ELD). Hypoxia was induced in mice by placing the animals in an airtight space at reduced pressure, and sodium nitrite poisoning was conducted for 1 hour after the last intragastric administration. The levels of glutathione peroxidase enzyme (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) in the myocardium and cerebral tissues were determined by the colorimetric method. Protein expression of BAX and BCL-2 was detected by ELISA. A high lethal dose (LD50) of 3,000 mg/kg was obtained in acute toxicity. Mice in the blank control group survived longer than those in the EHD group under hypoxic conditions. All biochemical criteria were significantly enhanced (P< 0.05). EEOO increased the tolerance of mice to hypoxia, which is indicative of its anti-hypoxia effects. The major components are 5,7-Dihydroxy-4'-methoxy-hydroxy-2-phenyl, 5,7-Dihydroxy-4'-methoxy-2-phenyl-4-benzo pyrone-3-O-b-galacto pyranoside.

  1. Allen R. G. and Tresini M. (2000) Oxidative stress and gene regulation. Free Radic. Biol. Med. 28: 463-499. 

  2. Editorial committee of Chinese Materia Medica (1999) Chinese Materia Medica (the volume of the Tibetan medicines). Shanghai Science and Technology Press., Shanghai, China.

  3. Hua H. and Zhao J. (2008) Research progresses in chemical properties, pharmacology and clinical application of Oxytrosis. Chinese Journal of Ethnomedicine and Ethnopharmacy 8: 30-34. 

  4. Institute for Drug and Biological Products Control, the Ministry of Health. (1984) Medicinal Herbal in Chinese Nation . Beijing, China: People’s Medical Publishing House.

  5. Kou Y. Y., ZhaXi D. J., Li Y. F., and Ge R. L. (2008) Antioxygenation of Tibetan medicine-sanwei tanxiang powder-on reperfusion injuries of mice induced by myocardial ischemia. Traditional Chinese Medicinal Materials 7: 1013-1015.

  6. Li Q., Li J. P., Tao S. L., Ding Y. J., He L., and Wang Z. (2009) Toxicity, damages, prophylaxis and treatment of Oxytropis. Pratacultural Science 26: 99-105.

  7. Liu S. S. (1997) Generating, partitioning, targeting and function of superoxide in mitochondria. Biosci. Rep. 17: 259-272.

  8. Li Y. L. (1998) Studies on the chemical components of Oxytropis deflexa. Traditional Chinese Medicinal Materials 29: 149.

  9. Li Z. W., Luo X. Q., Cai C., Zhang X. H. and Jin L. H. (2012) Research processes in anti-hypoxia effects and the mechanisms of the flavonoids compounds. Journal of Jilin Medical College 2: 111-113. 

  10. Misonou H., Menegola M., Buchwalder L., Park E. W., Meredith A., Rhodes K. J., Aldrich R. W. and Trimmer J. S. (2006) Immunolocalization of the Ca2+-activated K+ channel Slol in axons and nerve terminals of mammalian brain and cultured neurons. J. Comp. Neurol. 496: 289-302.

  11. Que S. (2007). Studies on the chemical components of Oxytropis falcata Bunge, a kind of Tibetan Medicines. Traditional Chinese Medicinal Materials, 38:14-58.

  12. Segall S., Artz W., Raslan D., Ferraz V., Takahashi J. (2005) Analysis of triacylglycerol isomers in Malaysian cocoa butter using HPLC-mass spectrometry, Food research international, 38: 167 - 174.

  13. Shen G. P., Kou Y. Y. and Li Y. F. (2011) The hemodynamic effect of 95% alcohol-extract from Tibetan herb bawei chenxiang powder on myocardial ischemia induced by pituitrin in rats. Journal of Qinghai Medical College 32: 231-234.

  14. Sun R. Q. (1987) Studies on the chemical components of Oxytropis ochrorephala Bunge-Part II: Structure of two kinds of triterpenoid saponin. Acta Chimica Sinica 45: 145.

  15. Van der Oost R., Beyer J. and Vermeulen N. P. (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 13: 57-149.

  16. Velioglu, Y. S., Mazza G., Gao L., and Oomah B. D. (1998) Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J. Agr. Food Chem. 46: 4113-4117.

  17. Wang D, Yang H, Yang GM, et al.(2010). Studies on the volatile components of Oxytropis falcata Bunge, a kind of Tibetan Medicines. Natural Product Research and Development, 22: 614-619.

  18. Xin H. L., Hou Y. H., Yue X. Q., Sheng J. Y., Li M., Lu J. C. and Ling C. Q. (2009) Screening of anti-hypoxia active sites in purslane and the related studies on its chemical components. Traditional Chinese Medicinal Materials 40: 114-    116.

  19. Yang G. M., Wang D., Tang W. and Cai B. C. (2010) Anti-inflammatory and antioxidant activities of Oxytropis falcata and its possible anti-inflammatory mechanism. Chinese Journal of Natural Medicines 8: 285-292.

  20. ZhaXi D. Z., Li X. J. and Que S. (2008) Preliminary experimental studies on anti-hypoxia effects of Oxytropis falcata Bunge, a kind of Tibetan Medicines. Journal of Qinghai Medical College 29: 266-273.

  21. ZhaXi D. Z., Li X. J. and Tong L. (2006) General introduction of Oxytropis falcata Bunge, a kind of Tibetan medicines. Journal of Qinghai Medical College 27: 137-138.


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