Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 4 (august 2013) : 331-337

EFFECT OF INOCULATION METHODS OF MESORHIZOBIUM CICERI AND PGPR IN CHICKPEA (CICER AREIETINUM L.) ON SYMBIOTIC TRAITS, YIELDS, NUTRIENT UPTAKE AND SOIL PROPERTIES

S
Sudeshna Bhattacharjya
R
Ramesh Chandra*
1Department of Soil Science, G. B. Pant University of Agriculture & Technology, Pantnagar – 263 145, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Bhattacharjya Sudeshna, Chandra* Ramesh (2025). EFFECT OF INOCULATION METHODS OF MESORHIZOBIUM CICERI AND PGPR IN CHICKPEA (CICER AREIETINUM L.) ON SYMBIOTIC TRAITS, YIELDS, NUTRIENT UPTAKE AND SOIL PROPERTIES. Legume Research. 36(4): 331-337. doi: .

ABSTRACT

Inoculation effects of Mesorhizobium ciceri (CH-1233) and PGPR (Pseudomonas sp., LK-884) alone and in combinations with different methods (seed treatment, furrows application by mixing with soil and vermicompost) in chickpea (Cicer arietinum L.) were compared during rabi season of 2008-09 at Pantnagar. Mesorhizobium sp. and PGPR alone inoculation significantly increased nodule number, by 44.3 and 34.4, nodule dry weight, by 46.1 and 33.2 %, and plant dry weight, by 33.6 and 42.4 %, over uninoculated control. Their co-inoculantion gave significant increases in nodule number of 16.5 % over PGPR alone and in nodule dry weight of 36.4 % over Mesorhizobium sp. alone inoculation. This treatment also significantly improved the plant dry weight by 11.5% over Mesorhizobium sp. alone inoculation. Mesorhizobium sp. alone recorded significant increase in grain yield of 11.8 % and PGPR alone in grain and straw yields of 15.3 and 14.9 % over uninoculated control. Their dual inoculation resulted in significant increase only in straw yield of 16.6 % over Mesorhizobium sp. alone. Co- inoculation of Mesorhizobium sp. and PGPR also significantly increased N uptake by straw and available P, dehydrogenase activity (DHA) and microbial biomass carbon (MBC) in soil over Mesorhizobium sp. alone inoculation. Inoculants applied in furrows mixing with soil and vermicompost resulted in better nodulation than seed treatment. These treatments also recorded significantly more N uptake of 17.7 and 14.5 % and P uptake of 12.5 and 16.0 % by straw over seed treatment, respectively.  Application of inoculants in furrows by mixing with soil was better than that mixing with vermicompost by recording significantly more available P, DHA and MBC in soil than seed treatment.

REFERENCES

  1. Alikhani, H. A., Saleh Rastin, N. and Antoun, H. (2006). Phosphate solubilization activity of rhizobia native to Iranian soils. Pl. Soil, 287:35-41.
  2. Bhatt, Parul and Chandra, R. (2009). Interaction of Mesorhizobium ciceri and rhizospheric bacteria on nodulation, growth and yield of chickpea. J. Food Legumes, 22:137-139.
  3. Burton, J.C. and Curley, R.L. (1965). Comparative efficiency of liquid and peat based inoculants on field grown soybeans. Agron. J., 57:379-381.
  4. Dadarwal, K.R. and Sen, A.N. (1973). Inhibitory effect of seed diffusates of some legumes on rhizobia and other bacteria. Indian J. Agric. Sci., 43:82-87.
  5. Deaker, R., Roughley, R.J and Kennedy, I. R. (2004). Legume seed inoculation technology - a review. Soil Biol. Biochem., 36:1275–1288.
  6. Dudeja, S.S., Singh, N.P., Poonam Sharma, Gupta, S.C., Ramesh Chandra, Bansi Dhar,. Bansal, R.K., Brahmaprakash, G.P., Potdukhe, S.R., Gundappagol, R.C., Gaikawad, B.G. and Nagaraj, K.S. (2011). Biofertilizer Technology and Pulse Production. In: Bioaugmentation, Biostimulation and Biocontrol. (A. Singh et al. Eds.), Springer- Verlag Berlin Heidelberg, pp 43-63.
  7. Gan,Y., Selles, F., Hanson, K.G., Zentner, R.P., McConkey, B.G. and McDonald, C.L. (2005). Effect of formulation and placement of Mesorhizobium inoculants for chickpea in the semiarid Canadian prairies. Can. J. Pl. Sci., 85:555-560.
  8. Glick, B.R. (1995). The enhancement of plant growth by free living bacteria. Can. J. Microbiol., 41:109-117.
  9. Gupta, S.C. (2006). Effect of combined inoculation on nodulation nutrient uptake and yield of chickpea in Vertisol. J. Indian Soc. Soil Sci., 54:251-25.
  10. Henri, F., Laurette, N. N., Annette, D., John, Q., Wolfgang, M., François-Xavier, E. and Dieudonné, N. (2008). Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon. Afr. J. Microbiol. Res., 2:171-178.
  11. Jenkinson, D.S. and Powlson, D.S. (1976). The effect of biological treatments on metabolism in soil. V. A method for measuring soil biomass. Soil. Biol. Biochem., 8:209-213.
  12. Jensen, E.S. (1987). Inoculation of pea by application of Rhizobium in planting furrow. Pl. Soil, 97:63-70.
  13. Kannna, V., Sharma, Poonam and Sharma, S. (2011). Studies on synergism between rhizobium amd plant growth promoting rhizobacteria in lentil (Lens culinaris, Medikus). J. Food Legumes, 24:158-159.
  14. Khurana, A.L.; and Dudeja S.S. (1981). Response of chickpea to Rhizobium and nitrogen on nitrogen fixation and grain yield. Pulse Crops Newsletter, 1: 105.
  15. Kloepper, J.W., Zablotowich, R.M., Tripping, E.M. and Liftshiz, R. (1991). Plant growth promotion mediated by bacterial rhizospehere In: The Rhizosphere and Plant Growth. (Keister, D.L. and Cregan, P.B. Eds.). Kluwer Academic Publishers, Dordrecht. pp. 315-326.
  16. Kyei-Boahen, S., Slinkard, A E. and Walley, F.L. (2002). Evaluation of rhizobial inoculation methods for chickpea. Agron. J., 94:851-859.
  17. Lifshitz, R., Kloepper, I.W. and Kozlowski, H. (1987). Growth promotion of canola (rapeseed) seedling by a strain of Pseudomonas putida under genobiotic conditions. Can. J. Microbiol., 33:390-395.
  18. Page, A.L. (1982). Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties (2nd ed). ASA and SSSA. Madison, Wisconsin USA., pp. 1158.
  19. Peix, A., Rivas-Boyero, A. A., Mateos, P. F., Rodriguez-Barrueco, C., Martinez-Molina, E. and Velazquez, E. (2001). Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol. Biochem., 33:103–110.
  20. Rodriguez, H. and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech. Adv., 17:319–339.
  21. Rupela, O.P. (1990). A visual rating system for nodulation of chickpea. ICN, 22:22-25.
  22. Sahai, P. and Chandra, R. (2011). Performance of Liquid and Carrier based Inoculants of Mesorhizobium ciceri and PGPR (Pseudomonas diminuta) in Chickpea (Cicer arietinum L.) on Nodulation, Yield and Soil properties. J. Indian Soc. Soil Sci., 59:263-267.
  23. Saini, V.K., Bhandari, S.C. and Tarafdar, J. C. (2004). Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crops Res., 89:39-47.
  24. Tabatabai, M.A. (1982). Soil Enzymes. In: Soil Analysis. Part 2. Chemical and Microbiological Properties 2nd edition (Page, A.L., Miller, R.H. and Keeney, D.R., Eds). American Society of Agronomy: Madison, Wisconsin; Agron. Monograph. 9: 937–940.
  25. Vance, E.D., Brookes, P.C. and Jenkinson, D.S. (1987). An extraction method for measuring the soil microbial biomass C. Soil. Biol. Biochem., 17:703-707.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 36 issue 4 (august 2013) : 331-337

    EFFECT OF INOCULATION METHODS OF MESORHIZOBIUM CICERI AND PGPR IN CHICKPEA (CICER AREIETINUM L.) ON SYMBIOTIC TRAITS, YIELDS, NUTRIENT UPTAKE AND SOIL PROPERTIES

    S
    Sudeshna Bhattacharjya
    R
    Ramesh Chandra*
    1Department of Soil Science, G. B. Pant University of Agriculture & Technology, Pantnagar – 263 145, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Bhattacharjya Sudeshna, Chandra* Ramesh (2025). EFFECT OF INOCULATION METHODS OF MESORHIZOBIUM CICERI AND PGPR IN CHICKPEA (CICER AREIETINUM L.) ON SYMBIOTIC TRAITS, YIELDS, NUTRIENT UPTAKE AND SOIL PROPERTIES. Legume Research. 36(4): 331-337. doi: .

    ABSTRACT

    Inoculation effects of Mesorhizobium ciceri (CH-1233) and PGPR (Pseudomonas sp., LK-884) alone and in combinations with different methods (seed treatment, furrows application by mixing with soil and vermicompost) in chickpea (Cicer arietinum L.) were compared during rabi season of 2008-09 at Pantnagar. Mesorhizobium sp. and PGPR alone inoculation significantly increased nodule number, by 44.3 and 34.4, nodule dry weight, by 46.1 and 33.2 %, and plant dry weight, by 33.6 and 42.4 %, over uninoculated control. Their co-inoculantion gave significant increases in nodule number of 16.5 % over PGPR alone and in nodule dry weight of 36.4 % over Mesorhizobium sp. alone inoculation. This treatment also significantly improved the plant dry weight by 11.5% over Mesorhizobium sp. alone inoculation. Mesorhizobium sp. alone recorded significant increase in grain yield of 11.8 % and PGPR alone in grain and straw yields of 15.3 and 14.9 % over uninoculated control. Their dual inoculation resulted in significant increase only in straw yield of 16.6 % over Mesorhizobium sp. alone. Co- inoculation of Mesorhizobium sp. and PGPR also significantly increased N uptake by straw and available P, dehydrogenase activity (DHA) and microbial biomass carbon (MBC) in soil over Mesorhizobium sp. alone inoculation. Inoculants applied in furrows mixing with soil and vermicompost resulted in better nodulation than seed treatment. These treatments also recorded significantly more N uptake of 17.7 and 14.5 % and P uptake of 12.5 and 16.0 % by straw over seed treatment, respectively.  Application of inoculants in furrows by mixing with soil was better than that mixing with vermicompost by recording significantly more available P, DHA and MBC in soil than seed treatment.

    REFERENCES

    1. Alikhani, H. A., Saleh Rastin, N. and Antoun, H. (2006). Phosphate solubilization activity of rhizobia native to Iranian soils. Pl. Soil, 287:35-41.
    2. Bhatt, Parul and Chandra, R. (2009). Interaction of Mesorhizobium ciceri and rhizospheric bacteria on nodulation, growth and yield of chickpea. J. Food Legumes, 22:137-139.
    3. Burton, J.C. and Curley, R.L. (1965). Comparative efficiency of liquid and peat based inoculants on field grown soybeans. Agron. J., 57:379-381.
    4. Dadarwal, K.R. and Sen, A.N. (1973). Inhibitory effect of seed diffusates of some legumes on rhizobia and other bacteria. Indian J. Agric. Sci., 43:82-87.
    5. Deaker, R., Roughley, R.J and Kennedy, I. R. (2004). Legume seed inoculation technology - a review. Soil Biol. Biochem., 36:1275–1288.
    6. Dudeja, S.S., Singh, N.P., Poonam Sharma, Gupta, S.C., Ramesh Chandra, Bansi Dhar,. Bansal, R.K., Brahmaprakash, G.P., Potdukhe, S.R., Gundappagol, R.C., Gaikawad, B.G. and Nagaraj, K.S. (2011). Biofertilizer Technology and Pulse Production. In: Bioaugmentation, Biostimulation and Biocontrol. (A. Singh et al. Eds.), Springer- Verlag Berlin Heidelberg, pp 43-63.
    7. Gan,Y., Selles, F., Hanson, K.G., Zentner, R.P., McConkey, B.G. and McDonald, C.L. (2005). Effect of formulation and placement of Mesorhizobium inoculants for chickpea in the semiarid Canadian prairies. Can. J. Pl. Sci., 85:555-560.
    8. Glick, B.R. (1995). The enhancement of plant growth by free living bacteria. Can. J. Microbiol., 41:109-117.
    9. Gupta, S.C. (2006). Effect of combined inoculation on nodulation nutrient uptake and yield of chickpea in Vertisol. J. Indian Soc. Soil Sci., 54:251-25.
    10. Henri, F., Laurette, N. N., Annette, D., John, Q., Wolfgang, M., François-Xavier, E. and Dieudonné, N. (2008). Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon. Afr. J. Microbiol. Res., 2:171-178.
    11. Jenkinson, D.S. and Powlson, D.S. (1976). The effect of biological treatments on metabolism in soil. V. A method for measuring soil biomass. Soil. Biol. Biochem., 8:209-213.
    12. Jensen, E.S. (1987). Inoculation of pea by application of Rhizobium in planting furrow. Pl. Soil, 97:63-70.
    13. Kannna, V., Sharma, Poonam and Sharma, S. (2011). Studies on synergism between rhizobium amd plant growth promoting rhizobacteria in lentil (Lens culinaris, Medikus). J. Food Legumes, 24:158-159.
    14. Khurana, A.L.; and Dudeja S.S. (1981). Response of chickpea to Rhizobium and nitrogen on nitrogen fixation and grain yield. Pulse Crops Newsletter, 1: 105.
    15. Kloepper, J.W., Zablotowich, R.M., Tripping, E.M. and Liftshiz, R. (1991). Plant growth promotion mediated by bacterial rhizospehere In: The Rhizosphere and Plant Growth. (Keister, D.L. and Cregan, P.B. Eds.). Kluwer Academic Publishers, Dordrecht. pp. 315-326.
    16. Kyei-Boahen, S., Slinkard, A E. and Walley, F.L. (2002). Evaluation of rhizobial inoculation methods for chickpea. Agron. J., 94:851-859.
    17. Lifshitz, R., Kloepper, I.W. and Kozlowski, H. (1987). Growth promotion of canola (rapeseed) seedling by a strain of Pseudomonas putida under genobiotic conditions. Can. J. Microbiol., 33:390-395.
    18. Page, A.L. (1982). Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties (2nd ed). ASA and SSSA. Madison, Wisconsin USA., pp. 1158.
    19. Peix, A., Rivas-Boyero, A. A., Mateos, P. F., Rodriguez-Barrueco, C., Martinez-Molina, E. and Velazquez, E. (2001). Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biol. Biochem., 33:103–110.
    20. Rodriguez, H. and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech. Adv., 17:319–339.
    21. Rupela, O.P. (1990). A visual rating system for nodulation of chickpea. ICN, 22:22-25.
    22. Sahai, P. and Chandra, R. (2011). Performance of Liquid and Carrier based Inoculants of Mesorhizobium ciceri and PGPR (Pseudomonas diminuta) in Chickpea (Cicer arietinum L.) on Nodulation, Yield and Soil properties. J. Indian Soc. Soil Sci., 59:263-267.
    23. Saini, V.K., Bhandari, S.C. and Tarafdar, J. C. (2004). Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crops Res., 89:39-47.
    24. Tabatabai, M.A. (1982). Soil Enzymes. In: Soil Analysis. Part 2. Chemical and Microbiological Properties 2nd edition (Page, A.L., Miller, R.H. and Keeney, D.R., Eds). American Society of Agronomy: Madison, Wisconsin; Agron. Monograph. 9: 937–940.
    25. Vance, E.D., Brookes, P.C. and Jenkinson, D.S. (1987). An extraction method for measuring the soil microbial biomass C. Soil. Biol. Biochem., 17:703-707.
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)