Legume Research-An International Journal

Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences

DOI: 10.18805/lr.v0iOF.11184    | Article Id: LR-260 | Page : 665-673
Citation :- Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences .Legume Research-An International Journal.2016.(39):665-673

Süleyman AVCI1*, Nilgün Tekin 2, Cengiz SANCAK 3, Sebahattin ÖZCAN3 and Amir Orojpour Marangi4

savci@ymail.com
Address :

Department of Field Crops, Faculty of Agriculture, Eskisehir Osmangazi University, Eskisehir, Turkey.

Submitted Date : 19-12-2015
Accepted Date : 26-05-2016
First Online:

Abstract

Phylogenetic relationships were studied based on morphology and nuclear ribosomal DNA internal transcript spacer (nrDNA ITS) sequences in 49 populations of 36 Onobrychis taxa belonging to 5 sections (Dendobrychis, Lophobrychis, Onobrychis, Heliobrychis and Hymenobrychis) naturally grown in Turkey. The morphological data (46 quantitative characters) were standardized and submitted for PCA analysis before the performance of hierarchical clustering analysis. In addition, a 577-bp region of the nrDNA ITS sequences was compared to generate a dendrogram using the neighbor-joining method. According to the obtained results, the Onobrychis genus is clearly divided into the Onobrychis and Sisyrosema subgenera both molecularly and morphologically; however, in terms of molecular data, while the Onobrychis subgenus showed polyphyletic features, the Sisyrosema subgenus had monophyletic features. Additionally, long-wing taxa belonging to Dendobrychis and Onobrychis and O. crista-galli belonging to Lophobrychis located in the same clade in Onobrychis showed polyphyletic features. This finding was confirmed in terms of long-wing taxa as well as morphology and indicated that these three sections were entangled with each other under the Onobrychis subgenus.

Keywords

Morphologic nrDNA ITS Onobrychis Phylogenetic analysis.

References

  1. Abou-El-Enain, M.M. (2002). Chromosomal criteria and their phylogenetic implications in the genus Onobrychis Mill. Sect. Lophobrychis (Leguminosae) with special reference to Egyptian species. Bot. J. Linn. Soc., 39: 409-414.
  2. Ahangarian, S., Osaloo, S.K. and Maassoumi, A.A. (2007). Molecular phylogeny of the tribe Hedysareae with special reference to Onobrychis (Fabaceae) as inferred from nrDNA ITS sequences. Iran. J. Bot., 13: 64-74.
  3. Aktoklu, E. (2001). Two new varieties and a new record in Onobrychis from Turkey. Turk. J. Bot., 25: 359-363.
  4. Arslan, E. and Ertugrul, K. (2010). Genetic relationships of the genera Onobrychis, Hedysarum, and Sartoria using seed storage proteins. Turk. J. Biol., 34: 67-73.
  5. Avci, S., Sancak, C., Can, A., Acar, A. and Pinar, N.M. (2013). Pollen morphology of the genus Onobrychis (Fabaceae) in Turkey. Turk. J. Bot., 37: 669-681.
  6. Avci, S., Ilhan, E., Erayman, M. and Sancak, C. (2014). Analysis of Onobrychis genetic diversity using SSR marker from related legume species. J. Anim. Plant Sci., 24: 556-566.
  7. Cenci, C.A., Bassi, G., Ferranti, E. and Romano, B. (2000). Some morphometric, anatomical and biochemical characteristics of fruits and seeds of Onobrychis spp. in Italy. Plant Biosyst., 134: 91-98.
  8. Davis, P.H., Mill, R.R. and Kit, T. (1988). Flora of Turkey and the East Aegean Islands. Edinburg University Press, Edinburgh, Vol: 10.
  9. Demdoum, S., Munoz, F., Delgado, I., Valderrabano, J. and Wunsch, A. (2012). EST-SSR cross-amplification and genetic similarity in Onobrychis genus. Genet. Resour. Crop Ev., 59: 253-260.
  10. Duman, H. and Vural, M. (1990). New taxa from South Anatolia. Doga TU. Botanik Dergisi 14: 45-48.
  11. Dündar, E., Akçiçek, E., Dirmenci, T. and Akgün, S. (2013). Phylogenetic analysis of the genus Stachys sect. Eriostomum (Lamiaceae) in Turkey based on nuclear ribosomal ITS sequences. Turk. J. Bot., 37: 14-23.
  12. Emre, I., Turgut-Balik, D., Sahin, A. and Kursat, M. (2007). Total electrophoretic band patterns of some Onobrychis species growing in Turkey. Am. Eurasian J. Agric. Environ Sci., 2: 123-126.
  13. Hayot Carbonero, C., Carbonero, F., Smith, L.M.J. and Brown, T.A. (2012). Phylogenetic characterisation of Onobrychis species with special focus on the forage crop Onobrychis viciifolia. Genet. Resour. Crop Ev., 59: 1777-1788.
  14. Hedge, I.C. (1970). Flora of Turkey and the East Aegean Islands. In: Flora of Turkey Vol. 3: Onobrychis. (Ed Davis PH) Edinburg University Press. Edinburgh, UK. pp: 560-589.
  15. Hejazi, H., Mohsen, S. and Nasab, M.Z. (2010). Cytotaxonomy of some Onobrychis (Fabaceae) species and populations in Iran. Caryologia, 63: 18-31.
  16. Karamian, R., Behjou, A.M. and Ranjbar, M. (2012). Anatomical findings of Onobrychis sect. Heliobrychis (Fabaceae) in Iran and their taxonomic implications. Turk. J. Bot., 36: 27-37.
  17. Lewke Bandara N., Papini, A., Mosti, S., Brown, T. and Smith, L.M.J. (2013): A phylogenetic analysis of genus Onobrychis and its relationships within the tribe Hedysareae (Fabaceae). Turk. J. Bot., 36: 27-37.
  18. Negri, V. and Cenci, C.A. (1988). Morphological characterisation of natural populations of Onobrychis viciifolia (Leguminosae) from Central Italy. Willdenowia, 17: 19-31.
  19. Safaei Chaei Kar, S., Ghanavati, F., Mozafari, J., Naghavi, M.R., Amirabadizadeh, H. and Darvish, F. (2012). Phylogenetic relationships of Onobrychis Mill. (Fabaceae: Papilionoideae) based on ITS sequences of nuclear ribosomal DNA and morphological traits. Crop Breed. J., 2: 91-99.
  20. Saitou, N. and Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406-425.
  21. Sepet, H., Emre, I., Kiran, Y., Kursat, M. and Sahin, A. (2011). Karyological studies on eight species of Onobrychis genus in Turkey. Biologia, 66: 996-100.
  22. Somay Akçelik, E., Avci, S., Uzun, S. and Sancak, C. (2012). Karyotype analysis of some Onobrychis (Sainfoin) species in Turkey. Arch. Biol. Sci., 64: 567-571.
  23. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol., 30: 2725-2729.
  24. Toluei, Z., Atri, M., Ranjbar, M. and Wink, M. (2012). Morphological, genetical and ecogeographical characterization of long-winged species of Onobrychis sect. Onobrychis (Fabaceae) in Iran. Iran. J. Bot., 18: 31-41.
  25. Yildiz, B., Çiplak, B. and Aktoklu, E. (1999). Preliminary phylogeny of sections of genus Onobrychis Miller (Fabaceae) with references of fruit morphology. Isr. J. Plant Sci., 47: 269-282.
  26. White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990). Amplification and direct sequencing of Fungal ribosomal RNA gene for phylogenetics. In: PCR protocols: a guide to methods and amplifications, M. Innis. J. Sninsky and T. White (eds.). Sand Diego, California: Academic press, pp. 315-322.
     

Global Footprints