The effects of spraying methanol solution and drought stress on level of antioxidant enzyme activity,  growth, biomass and yield of beans (Phaseolus vulgaris

DOI: 10.18805/lr.v39i3.10752    | Article Id: LR-242 | Page : 385-395
Citation :- The effects of spraying methanol solution and drought stress on level of antioxidant enzyme activity, growth, biomass and yield of beans(Phaseolus vulgaris .Legume Research-An International Journal.2016.(39):385-395

Nezam Armand, Hamzeh Amiri* and Ahmad Ismaili1

Address :

Department of Biology, Faculty of Sciences, Lorestan University, Iran.

Submitted Date : 27-06-2015
Accepted Date : 1-06-2016


An experiment was conducted to investigate the effect of methanol solution spray treatment on growth, biomass, and functional properties of bean plant under drought stress. The experiment was set up as a fully-randomized design with three replications. The first factor was four concentration levels of spray solution (Control, 10, 20, and 30%) and the spray was applied three times during the plant growth season at 10-day intervals. The second factor was three levels of drought stress; severe drought stress (25% field capacity), moderate drought stress (75% field capacity), and non-stress (100% field capacity). Test results showed that under 20% of methanol and conditions of non-stress there was significant growth of protein content of root and leaf in comparison with the control. Antioxidant enzyme activity was not affected by application of methanol solution spray but leaf antioxidant enzymes activity declined. Under non-stress and methanol concentrations of 10 and 20%, results showed a significant increase in all morphological properties compared with the control treatment. Under conditions of severe and moderate drought stress, level of methanol solution spray did not mitigate the negative effects of drought stress on the studied properties. 


Antioxidant activity Methanol foliar application Root yield Water stress.


  1. Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y. and Sakuratani, T. (2002). Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant. Sci. 163: 117-123. 
  2. Ahmadpour, R., Hosseinzadeh, S.R., Armand, N. and Fani, E. (2015). Effect of methanol on germination characteristics of lentil (Lens culinaris Medik.) under drought stress. Iranian J.Seed. Res. 2: 83-96.
  3. Asada, K., Foyer, C. and Mullineaux, P. (1994). Production and action of active oxygen species in photosynthetic tissues. Causes of photooxidative stress and amelioration of defense systems in plants. J. Bio. 10: 77-104.
  4. Ashraf, M. and Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166: 3-16. 
  5. Bagheri, A., Mahmoudi, A. and Ghezeli, F. (2001) Common Bean: Research For Crop Improvement. Jahad daneshgahi, Iran, 2001.
  6. Beauchamp, C. and Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal.biochem. 44: 276-287.
  7. Benson, A.A. and Nonomura, A.M. (1994). The path of carbon in photosynthesis: methanol inhibition of glycolic acid accumulation. Photosynthesis. Res. 34: 196-200.
  8. Blum, A. (1996). Crop responses to drought and the interpretation of adaptation. Plant Growth Regul. 20: 135-148. 
  9. Bor, M., Özdemir, F. and Türkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant. Sci. 164: 77-84.
  10. Dat, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D. and Van Breusegem, F. (2000). Dual action of the active oxygen species during plant stress responses. Cel & Mol. Life Sci. 57: 779-795.
  11. Díaz, P., Monza, J. and Márquez (2005). A. Drought and saline stress. In: [A. Márquez, editor] Lotus japonicus Handbook. Springer Netherlands.
  12. Dorri, H.R. (2008). Bean Agronomy. Publication Series of Research Center of Bean, Khomein.
  13. Downie, A., Miyazaki, S., Bohnert, H., John, P., Coleman, J. and Parry, M. (2004). Expression profiling of the response of Arabidopsis thaliana to methanol stimulation. Phytochem. 65: 2305-2316. 
  14. Ehyaei, H.R., Parsa, M., Kafi, M. and Nasiri mahalati, M. (2010). Effect of foliar application of methanol and irrigation regimes on yield and yield components of chickpea cultivars. Iranian J. Puls. Res. 11: 37-48.
  15. Eraslan, F., Inal, A., Savasturk, O. and Gunes, A. (2007). Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity. Scientia Horticul. 114: 5-10. 
  16. Farzad, P., Mohammad, N., Moghadam, H.R.T., Hossein, Z. and Alahmadi, M.J. (2007). Effects of drought stress on chlorophyll fluorescence parameters, chlorophyll content and grain yield of wheat cultivars. J. Biol. Sci. 11:66-83.
  17. Faver, K. and Gerik, T. (1996). Foliar-applied methanol effects on cotton (Gossypium hirsutum L.) gas exchange and growth. Field. Crop. Res. 47: 227-234.
  18. Galbally, I.E. and Kirstine, W. (2002). The production of methanol by flowering plants and the global cycle of methanol. J. Atmospher. Chem. 43: 195-229. 
  19. Gout, E., Aubert, S., Bligny, R., Rebeille, F., Nonomura, A.R. and Benson, A.A. (2000). Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies. Plant physiol. 123: 287-296.
  20. Heins, R. (1980). Inhibition of ethylene synthesis and senescence in carnation by ethanol. J. Ameri. Soc. Horti.Sci. 105: 141-144.
  21. Hosseinzadeh, S.R., Cheniany, M. and Salimi, A. (2014). Effects of foliar application of methanol on physiological characteristics of chickpea (Cicer arietinum L.) under drought stress. Iranian J. Puls. Res. 5: 71-82.
  22. Hosseinzadeh, S.R., Amiri, H. and Ismaili, A. (2016). Effect of vermicompost fertilizer on photosynthetic characteristics of chickpea (Cicer arietinum L.) under drought stress. Photosynthetica. 54: 87-92.
  23. Hosseinzadeh, S.R., Salimi, A., Ganjeali, A. and Ahmadpour, R. (2012). Effects of foliar application of methanol on growth and root characteristics of chickpea (Cicer arietinum L.) under drought stress. European J. Experiment. Bio.2:1697-1702.
  24. Hoyle, M.C. (1972). Indoleacetic Acid oxidase: a dual catalytic enzyme? Plant physiol. 50: 15-18.
  25. Ivanova, E., Doronina, N. and Trotsenko, Y.A. (2001). Aerobic methylobacteria are capable of synthesizing auxins. Microbiology. 70: 392-397.
  26. Khafagi, W.I. (1997). Effect of different irrigation intervals on sugar beet growth, plant water relations and photosynethetic pigments. Annal. Agri. Sci. 33: 305-319.
  27. Khosravi, M.T., Mehrafarin, A., Naghdibadi, H., Hajiaghaee, R. and Khosravi, E. (2011). Effect of methanol and ethanol application on yield of Echinacea purpurea L. in Karaj region. J. Herbal. Drugs. 2: 121-128.
  28. Lee, H.S., Madhaiyan, M., Kim, C.W., Choi, S.J., Chung, K.Y. and Sa, T.M. (2006). Physiological enhancement of early growth of rice seedlings (Oryza sativa L.) by production of phytohormone of N2-fixing methylotrophic isolates. Biol Fertil Soils 42: 402-408. 
  29. Leport, L., Turner, N., Davies, S.L., Siddique, K., Leport, L., Turner, N. (2006). Variation in pod production and abortion among chickpea cultivars under terminal drought. Eur. J. Agron. 24: 236-246. 
  30. Li, Y., Gupta, G., Joshi, J.M. and Siyumbano, A.K. (1995). Effect of methanol on soybean photosynthesis and chlorophyll. J. Plant Nut. 18: 1875-1880. 
  31. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the folin phenol reagent. J. Bio. Chem. 193: 265-275.
  32. Madhaiyan, M., Poonguzhali, S., Sundaram, S.P. and Sa, T. (2006). A new insight into foliar applied methanol influencing phylloplane methylotrophic dynamics and growth promotion of cotton (Gossypium hirsutum L.) and sugarcane (Saccharum officinarum L.). Environ & Experi. Bot. 57: 168-176. 
  33. Makhdum, I.M., Nawaz, A., Shabab, M., Ahmad, F. and Illahi, F. (2002). Physiological response of cotton to methanol foliar application. J. Res. Pak. 13: 37-43.
  34. Maliti, C.M. (2000). Physiological and Biochemical Effects of Methylobacterium Sp. Strains and Foliar-applied Methanol on Growth and Development of Rice Oryza Sativa L. City University of New York.
  35. Moussa, H.R. and Abdel-Aziz, S.M. (2008). Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Aust. J. Crop. Sci. 1: 31-36.
  36. Najaphy, A., Khamssi, N.N., Mostafaie, A. And Mirzaee, H. (2010). Effect of progressive water deficit stress on proline accumulation and protein profiles of leaves in chickpea. African J. Biotech. 9: 7033-7036.
  37. Nemecek-Marshall, M., MacDonald, R.C., Franzen, J.J., Wojciechowski, C.L. and Fall, R. (1995). Methanol emission from leaves (enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomatal conductance and leaf development). Plant Physiol. 108: 1359-1368.
  38. Nonomura, A.M. and Benson, A.A. (1992). The path of carbon in photosynthesis: improved crop yields with methanol. Proceedings of the National Academy of Sciences of the United States of America 89: 9794-9798.
  39. Parsa, M. and Bagheri, A. (2008). Legumes. Mashhad University Jahad Press, Iran.
  40. Rahbarian, R., Khavari-nejad, R., Ganjeali, A., Bagheri, A.R. and Najafi, F. (2011). Drought stress effects on photosynthesis, chlorophyll fluorescence and water. Acta Biol. Craco. Bot. 53: 47-56.
  41. Ramadant, T. and Omran, Y. (2005). The effect of foliar application of methanol on productivity and fruit quality of grapevine cv. Flame Seedless. Vitis Journal. 44: 11-16.
  42. Ranalli, P., Candilo, M.d. and Bagatta, M. (1997). Drought tolerance screening for potato improvement. Plant. Breed. 116: 290-292. 
  43. Rowe, R.N., Farr, D.J. and Richards, B.A.J. (1994). Effects of foliar and root applications of methanol or ethanol on the growth of tomato plants (Lycopersicon esculentum.L). New Zealand. J. Crop & Horti. Sci. 22: 335-347.
  44. Safarzade Vishkaei (2007). M. Effects of methanol on growth and yield of peanut. Doctoral thesis Azad University of Agricultural Sciences rasht, Iran.
  45. Shao, H.B., Chu, L.Y., Lu, Z.H. and Kang, C. (2008). Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. Inter. J. Bio. Sci. 4: 8-15.
  46. Simova-Stoilova, L., Demirevska, K., Petrova, T., Tsenov, N. and Feller, U. (2008). Antioxidative protection in wheat varieties under severe recoverable drought at seedling stage. Plant Soil Environ. 54: 529-536.
  47. Yordanov, I., Velikova, V. and Tsonev, T. (2000). Plant responses to drought, acclimation, and stress tolerance. Photosynthetica. 38: 171-186. 
  48. Zbiec, I., Karczmarczyk, S. and Podsiadlo, C. (2003). Response of some cultivated plants to methanol as compared to supplemental irrigation. 6(1):1-. Electron. J. Agri. 6: 1-7.

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