Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 38 issue 3 (june 2015) : 313-320,   Doi: 10.5958/0976-0571.2015.00116.2

Molecular characterization of three hypersensitive-induced reaction genes that respond to Phytophthora sojae infection in Glycine max L. Merr.

Y
Yang Xiang
M
Min Song1
M
Minqin Zhang
S
Shifeng Cao2
H
Hongshi Han*
1Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550 008, P.R.China.
Cite article:- Xiang Yang, Song1 Min, Zhang Minqin, Cao2 Shifeng, Han* Hongshi (2025). Molecular characterization of three hypersensitive-induced reaction genes that respond to Phytophthora sojae infection in Glycine max L. Merr.. Legume Research. 38(3): 313-320. doi: 10.5958/0976-0571.2015.00116.2.

ABSTRACT

Hypersensitive-induced reaction (HIR) proteins, which are members of the proliferation, ion and death (PID) superfamily, have been shown to be involved in the defense responses of several plant species. In this study, three HIR-like genes from soybean, designated as GmHIR1, GmHIR3 and GmHIR4, were cloned and characterized. They each contained four introns and five exons and shared conservative structure of genomic organization. The deduced polypeptide sequences of GmHIRs share high degrees of similarity with HIR proteins of other species. However, QRT-PCR analysis indicated that the expression levels in different tissues were not the identical. The expression of GmHIRs were rapidly induced by Phytophthora sojae infection in the resistant genotype, while induction was very weak in the susceptible genotype. In addition, the expression of these genes were also repressed by hydrogen peroxide (H2O2) treatment. Promoter analysis indicates that several defense-responsive elements are present in the GmHIR promoter. To our knowledge, this is the first report on molecular cloning and initial characterization of HIR-like genes in soybean. This study may help elucidate soybean disease resistance, which would enhance efforts to control soybean Phytophthora root and stem rot disease.

REFERENCES

  1. Buchel, A.S., Brederode, F.T., Bol, J.F. and Linthorst, H.J. (1999). Mutation of GT-1 binding sites in the Pr-1A promoter influences the level of inducible gene expression in vivo. Plant Mol. Biol., 40: 387-396.
  2. Burnham, K.D., Dorrance, A.E., Francis, D.M., Fioritto, R.J. and Martin, S.K.S. (2003). Rps8, a new locus in soybean for resistance to Phytophthora sojae. Crop science., 43: 101-105.
  3. Claros, M.G. and von Heijne, G. (1994). TopPred II: an improved software for membrane protein structure predictions. Comput. Appl. Biosci.,10: 685-686.
  4. de Castro, E., Sigrist, C.J., Gattiker, A., Bulliard, V., Langendijk-Genevaux, P.S., Gasteiger, E., Bairoch, A. and Hulo, N. (2006). ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucl. Acids. Res., 34: W362-W365.
  5. Dangl, J.L. and Jones, J.D.G. (2001). Plant pathogens and integrated defense responses to infection. Nature., 411: 826-833.
  6. de los Reyes, B.G., Morsy, M., Gibbons, J., Varma, T.S., Antoine, W., McGrath, J.M., Halgren, R. and Redus, M. (2003). A snapshot of the low temperature stress transcriptome of developing rice seedlings (Oryza sativa L.) via ESTs from subtracted cDNA library. Theor. Appl. Genet., 107: 1071-1082.
  7. Duan, YH., Guo, J., Shi, X., Guan, X., Liu, F., Bai, P., Huang, L. and Kang, Z. (2013). Wheat hypersensitive-induced reaction genes TaHIR1 and TaHIR3 are involved in response to stripe rust fungus infection and abiotic stresses. Plant Cell Rep., 32: 273-283.
  8. Eulgem, T., Rushton, P.J., Schmelzer, E., Hahlbrock, K. and Somssich, I.E. (1999). Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J., 18: 4689-4699.
  9. Heath, M.C. (1998). Apoptosis, programmed cell death and the hypersensitive response. Eur. J. Plant Pathol., 104: 117-124.
  10. Heath, M.C. (2000). Hypersensitive response-related death. Plant Mol. Biol. 44: 321-334.
  11. Huang, X. and Madan, A. (1999). CAP3:A DNA sequence assembly program. Genome Res., 9: 868-877.
  12. Jones, J.D. and Dangl, J.L. (2006). The plant immune system. Nature., 444: 323-329.
  13. Jung, H.W. and Hwang, B.K. (2007). The leucine-rich repeat (LRR) protein, CaLRR1, interacts with the hypersensitive induced reaction (HIR) protein, CaHIR1, and suppresses cell death induced by the CaHIR1 protein. Mol. Plant Pathol., 8: 503-514.
  14. Karrer, E.E., Beachy, R.N. and Holt, C.A. (1998). Cloning of tobacco genes that elicit the hypersensitive response. Plant Mol. Biol., 36: 681-690.
  15. Kim, M.S., Kim, Y.C. and Cho, B.H. (2004). Gene expression analysis in cucumber leaves primed by root colonization with pseudomonas chlororaphis O6 upon challenge-inoculation with Corynespora cassiicola. Plant biol. (Stuttg.)., 6: 105-108.
  16. Krüger, J., Thomas, C.M., Golstein, C., Dixon, M.S., Smoker, M., Tang, S.J., Mulder, L. and Jones, J.D. (2002). A tomato cysteine protease required for Cf-2-dependent disease resistance and suppression of autonecrosis. Science., 296: 744-747.
  17. Lescot, M., Dehais, P., Thijs, G., Marchal, K., Moreau, Y., Van de Peer, Y., Rouze, P. and Rombauts, S. (2002). Plant CARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acid Res., 30: 325-327.
  18. Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods., 25: 402-408.
  19. Nadimpalli, R., Yalpani, N., Johal, G.S. and Simmons, C.R. (2000). Prohibitins, stomatins, and plant disease response genes compose a protein superfamily that controls cell proliferation, ion channel regulation, and death. J. Biol. Chem., 275: 29579-29586.
  20. Park, H.C., Kim, M.L., Kang, Y.H., Jeon, J.M., Yoo, J.H., Kim, M.C., Park, C.Y., Jeong, J.C., Moon, B.C., Lee, J.H., Yoon, H.W., Lee, S.H., Chung, W.S., Lim, C.O., Lee, S.Y., Hong, J.C. and Cho, M.J. (2004). Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor. Plant Physiol., 135: 2150-2161.
  21. Park, J.M. (2005). The hypersensitive response. A cell death during disease resistance. Plant Pathol. J., 21: 99-101.
  22. Qi, Y.P., Tsuda, K., Nguyen, L.V., Wang, X., Lin, J., Murphy, A.S., Glazebrook, J., Thordal-Christensen, H. and Katagiri, F. (2011). Physical association of Arabidopsis Hypersensitive Induced Reaction proteins (HIRs) with the immune receptor RPS2. J. Biol. Chem., 286: 31297-31307.
  23. Ren, J., Wen, L., Gao, X., Jin, C., Xue, Y. and Yao, X. (2008). CSS-Palm 2.0: an updated software for palmitoylation sites prediction. Protein Eng. Des. Sel., 21: 639-644.
  24. Rostoks, N., Schmierer, D., Kudrna, D. and Kleinhofs, A. (2003). Barley putative hypersensitive induced reaction genes: genetic mapping, sequence analyses and differential expression in disease lesion mimic mutants. Theor. Appl. Genet., 107: 1094-1101.
  25. Rushton, P.J., Torres, J.T., Parniske, M., Wernert, P., Hahlbrock, K. and Somssich, I.E. (1996). Interaction of elicitor- induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes. EMBO J., 15: 5690-5700.
  26. Sun, S., Zhao, J., Wu, X., Guo, N., Wang, Y., Gai, J. and Xing, H. (2008). Resistance of soybean germplasm to phytophthora in Huaihai Valley. Soybean Science., 27: 465-470.
  27. Tyler, B.M. (2007). Phytophthora sojae: root rot pathogen of soybean and model oomycete. Mol. Plant Pathol., 8: 1-8.
  28. Vieweg, M.F., Frühling, M., Quandt, H.J., Heim, U., Bäumlein, H., Pühler, A., Küster, H. and Andreas, M.P. (2004). The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants. Mol. Plant Microbe. Interact., 17: 62-69.
  29. Wu, X.L., Zhao, J.M., Wang, Y.L., Sun, S., Guo, N., Tang, Q.H., Wang, Y.C., Gai, J.Y. and Xing, H. (2009). Identification and isolation of soybean root rot in Nanjing. Journal of Nanjing Agricultural University., 32: 61-64.
  30. Yamamoto, S., Nakano, T., Suzuki, K. and Shinshi, H. (2004). Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochim. Biophys. Acta., 1679: 279-287.
  31. Yu, D., Chen, C. and Chen, Z. (2001). Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell., 13: 1527-1540.
  32. Yu, X.M., Yu, X.D., Qu, Z.P., Huang, X.J., Guo, J., Han, Q.M., Zhao, J., Huang, L.L. and Kang, Z.S. (2008). Cloning of a putative hypersensitive induced reaction gene from wheat infected by stripe rust fungus. Gene., 407: 193-198.
  33. Zhang, G., Dong, Y.L., Zhang, Y., Li, Y.M., Wang, X.J., Han, Q.M., Guo, J., Huang, L.L. and Kang, Z.S. (2009). Cloning and characterization of a novel hypersensitive-induced reaction gene from wheat infected by Stripe Rust Pathogen. J. Phytopathol., 157: 722-728.
  34. Zhou, L., Cheung, M.Y., Li, M.W., Fu, Y.P., Sun, Z.X., Sun, S.M. and Lam, H.M. (2010). Rice Hypersensitive Induced Reaction Protein 1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death. BMC Plant Biol., 10: 1-10.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)