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volume 37 issue 1 (february 2014) : 32-36,   Doi: 10.5958/j.0976-0571.37.1.005

IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016

X
Xiaohua Wu
B
Baogen Wang
Z
Zhongfu Lu
X
Xinyi Wu
G
Guojing Li
P
Pei Xu*
1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China
Cite article:- Wu Xiaohua, Wang Baogen, Lu Zhongfu, Wu Xinyi, Li Guojing, Xu* Pei (2025). IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016. Legume Research. 37(1): 32-36. doi: 10.5958/j.0976-0571.37.1.005.

ABSTRACT

Powdery mildew (PM), caused by the fungus Erysiphe polygoni D.C, is one of the most common and serious diseases of cowpea (Vigna. unguiculata L. Walp.)/asparagus bean (Vigna. unguiculata ssp. sesquipedialis). Deployment of scab-resistant varieties is the major strategy to curb this disease. ZN016, a Chinese asparagus bean landrace, is highly resistant to powdery mildew. To identify the resistance genes in ZN016, an F7:8 recombinant inbred line (RIL) population derived from the cross between ZN016 and a susceptible cultivar Zhijiang282 was evaluated for PM resistance in the open field in 2008 and 2009. Phenotypic analysis indicated that ZN016 harbors a single PM resistance gene. Using a SNP and SSR based genetic map constructed with this population, the PM resistance gene was mapped to a locus on LG9 flanked by SSR markers CLM0305 and CLM0260, with a genetic distance of 2.0 cM and 5.0 cM, respectively. To our knowledge, this is the first powdery mildew resistance gene reported in cowpea, which was temporarily designated as Vu-Pm1. The identification of Vu-Pm1 opens a door for dissecting resistance mechanisms and applying marker-assisted selection for the development of cultivars with PM resistance in cowpea.

REFERENCES

  1. Ainsworth GC (1971) Ainsworth and Bisby’s dictionary of the fungi. Commonwealth Mycological Institute, Kew, Surrey, UK
  2. Bassam BJ, Gaetano-AnolléG, GresshoV PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels.         Anal Biochem 196:80–83
  3. Ben-David R, Xie WL, Peleg Z, Saranga Y, Dinoor A, Fahima T (2010) Identification and mapping of PmG16, a             powdery mildew resistance gene derived from wild emmer wheat. Theor Appl Genet 121:499–510
  4. Chaitieng B, Kaga A, Han OK, Wang X, Wongkaew S, Laosuwan P, Tomooka N, Vaughan DA (2002) Mapping a new         source of resistance to powdery mildew in mungbean. Plant Breed 121:521–525
  5. Chankaew S, Somta P, Isemura T, Tomooka N, Kaga A, Vaughan DA, Srinives P (2013) Quantitative trait locus             mapping reveals conservation of major and minor loci for powdery mildew resistance in four sources of resistance         in mungbean (Vigna radiata L. Wilczek). Mol Breeding 32:121–130
  6. Chantret N, Sourdille P, Röder M, Tavaud M, Bernard M, Doussinault G (2000) Location and mapping of the powdery         mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with             microsatellites in wheat. Theor Appl Genet 100:1217–1224
  7. Emechebe AM, Florini DA (1997) Shoot and pod diseases of cowpea induced by fungi and bacteria. Pages 176–192 in         Advances in cowpea research, edited by Singh BB, Mohan Raj DR, Dashiell KE, Jackai LEN. Sayce Publishing,         Devon, UK
  8. Goncalves ECP, Mauro AOD, Centurion MAPC (2002) Genetics of resistance to powdery mildew (Microsphaera diffusa)         in Brazilian soybean populations. Genet Mol Biol 25:339–342
  9. Hawtin G, Iwanaga M, Hodgkin T (1996) Genetic resources in breeding for adaptation. Euphytica 92:255–266
  10. He R, Chang Z, Yang Z, Yuan Z, Zhan H, Zhang X, Liu J (2009) Inheritance and mapping of powdery mildew resistance         gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180
  11. Hua W, Liu Z, Zhu J, Xie C, Yang T, Zhou Y, Duan X, Sun Q (2009) Identification and genetic mapping of pm42, a new         recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides).         Theor Appl Genet 119:223–230
  12. Humphry ME, Magner T, McIntyre CL, Aitken EA, Liu CL (2003) Identification of major locus conferring resistance to         powdery mildew (Erysiphe polygoni D.C.) in mungbean (Vigna radiata L. Wiczek) by QTL analysis. Genome         46:738–744
  13. Jun TH, Rouf Mian MA, Michel AP (2012) Genetic mapping revealed two loci for soybean aphid resistance in PI             567301B. Theor Appl Genet 124:13–22
  14. Kang ST, Mian MA (2010) Genetic map of the powdery mildew resistance gene in soybean PI 243540. Genome 53:400–405
  15. Kasettranan W, Somta P, Srinives P (2010) Mapping of quantitative trait loci controlling powdery mildew resistance in         mungbean (Vigna radiata L. Wilczek). J Crop Sci Biotechnol 13(3):155–161
  16. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
  17. Li CY, Wei J, Lin YJ, Chen H (2012) Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new         paradigm in plant breeding. Plant Cell Rep 31:851–862
  18. Li G, Fang T, Zhang H, Xie C, Li H, Yang T, Nevo E, Fahima T, Sun Q, Liu Z (2009) Molecular identification of a new         powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var.         dicoccoides). Theor Appl Genet 119:531–539
  19. Li ZZ, Sanchez A, Angeles E, Singh S, Domingo J, Huang N, Khush GS (2001) Are the dominant and recessive plant         disease resistance genes similar?: A case study of rice R genes and Xanthomonas oryzae pv. oryzae races.         Genetics 159:757–765
  20. Lillemo M, Asalf B, Singh R, Huerta-Espino J, Chen X, He Z, Bjørnstad Å (2008) The adult plant rust resistance loci         Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat         line Saar. Theor Appl Genet 116:1155–1166
  21. Lin Mction , Rios GP (1985) Cowpea diseases and their prevalence in Latin America. Pages 199-204 in Cowpea             research, production and utilization, edited by Singh SR and Rachie KO, John Wiley and Sons, Chichester, UK
  22. Lohnes DG, Bernard RL (1992) Inheritance of resistance to powdery mildew in soybeans. Plant Dis 76:964–965
  23. Luo PG, Luo HY, Chang ZJ, Zhang HY, Zhang M, Ren ZL (2009) Characterization and chromosomal location of Pm40         in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor         Appl Genet 118:1059–1064
  24. Maguire T, Collins G, Sedgley M (1994) A modified CTAB DNA extraction procedure for plants belonging to the family         proteaceae. Plant Mol Biol Rep 12: 106–109
  25. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene         symbols for wheat: 2008 supplement. Annu Wheat Newsl 54:209–225. http://wheat.pw.usda.gov/GG2/pubs.shtml
  26. Mignucci JS, Lim SM (1980) Powdery mildew (Microspraera diffusa) development on soybeans with adult-plant resistance.         Phytopathology 70:919–921
  27. Muchero M, Diop NN, Bhat PR, Fenton RD, Wanamaker S, Pottorff M, Hearne S, Cisse N, Fatokun C, Ehlers JD,         Roberts PA, Close TJ  (2009) A consensus genetic map of cowpea [Vigna unguiculata L. Walp] and synteny         based on EST-derived SNPs. Proc Natl Acad Sci USA 106: 18159–18164
  28. Raju SG, Anilkumar TB (1990) Evaluation of cowpea genotypes for partial resistance to powdery mildew. Euphytica         50:191–195
  29. Raju SG, Anilkumar TB (1991) Sources of partial resistance to powdery mildew in cowpea. Archiv für Phytopathologie         and Pflanzenschutz 27:153–156
  30. Smart J (1976) Tropical pulses. Longman Group Ltd, London
  31. Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale         genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective         against multiple pathogens in wheat. Theor Appl Genet 116:481–490
  32. Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS         conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet             126:1397–1403
  33. Xu P, Wu XH, Wang BG, Liu RH, Ehlers JD, Close TJ, Roberts PA, Diop NN, Qin DH, Hu TT, Lu ZF, Li GJ (2011) A         SNP and SSR based genetic map of Asparagus Bean (Vigna. unguiculata ssp. sesquipedialis) and comparison         with the broader species. PLoS ONE 6(1): e15952. doi:10.1371/journal.pone.0015952
  34. Young ND, Danesh D, Menancio-Hautea D, Kumar L (1993) Mapping oligogenic resistance to powdery mildew in             mungbean with RFLPs. Theor Appl Genet 87:243–249
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    volume 37 issue 1 (february 2014) : 32-36,   Doi: 10.5958/j.0976-0571.37.1.005

    IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016

    X
    Xiaohua Wu
    B
    Baogen Wang
    Z
    Zhongfu Lu
    X
    Xinyi Wu
    G
    Guojing Li
    P
    Pei Xu*
    1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China
    Cite article:- Wu Xiaohua, Wang Baogen, Lu Zhongfu, Wu Xinyi, Li Guojing, Xu* Pei (2025). IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016. Legume Research. 37(1): 32-36. doi: 10.5958/j.0976-0571.37.1.005.

    ABSTRACT

    Powdery mildew (PM), caused by the fungus Erysiphe polygoni D.C, is one of the most common and serious diseases of cowpea (Vigna. unguiculata L. Walp.)/asparagus bean (Vigna. unguiculata ssp. sesquipedialis). Deployment of scab-resistant varieties is the major strategy to curb this disease. ZN016, a Chinese asparagus bean landrace, is highly resistant to powdery mildew. To identify the resistance genes in ZN016, an F7:8 recombinant inbred line (RIL) population derived from the cross between ZN016 and a susceptible cultivar Zhijiang282 was evaluated for PM resistance in the open field in 2008 and 2009. Phenotypic analysis indicated that ZN016 harbors a single PM resistance gene. Using a SNP and SSR based genetic map constructed with this population, the PM resistance gene was mapped to a locus on LG9 flanked by SSR markers CLM0305 and CLM0260, with a genetic distance of 2.0 cM and 5.0 cM, respectively. To our knowledge, this is the first powdery mildew resistance gene reported in cowpea, which was temporarily designated as Vu-Pm1. The identification of Vu-Pm1 opens a door for dissecting resistance mechanisms and applying marker-assisted selection for the development of cultivars with PM resistance in cowpea.

    REFERENCES

    1. Ainsworth GC (1971) Ainsworth and Bisby’s dictionary of the fungi. Commonwealth Mycological Institute, Kew, Surrey, UK
    2. Bassam BJ, Gaetano-AnolléG, GresshoV PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels.         Anal Biochem 196:80–83
    3. Ben-David R, Xie WL, Peleg Z, Saranga Y, Dinoor A, Fahima T (2010) Identification and mapping of PmG16, a             powdery mildew resistance gene derived from wild emmer wheat. Theor Appl Genet 121:499–510
    4. Chaitieng B, Kaga A, Han OK, Wang X, Wongkaew S, Laosuwan P, Tomooka N, Vaughan DA (2002) Mapping a new         source of resistance to powdery mildew in mungbean. Plant Breed 121:521–525
    5. Chankaew S, Somta P, Isemura T, Tomooka N, Kaga A, Vaughan DA, Srinives P (2013) Quantitative trait locus             mapping reveals conservation of major and minor loci for powdery mildew resistance in four sources of resistance         in mungbean (Vigna radiata L. Wilczek). Mol Breeding 32:121–130
    6. Chantret N, Sourdille P, Röder M, Tavaud M, Bernard M, Doussinault G (2000) Location and mapping of the powdery         mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with             microsatellites in wheat. Theor Appl Genet 100:1217–1224
    7. Emechebe AM, Florini DA (1997) Shoot and pod diseases of cowpea induced by fungi and bacteria. Pages 176–192 in         Advances in cowpea research, edited by Singh BB, Mohan Raj DR, Dashiell KE, Jackai LEN. Sayce Publishing,         Devon, UK
    8. Goncalves ECP, Mauro AOD, Centurion MAPC (2002) Genetics of resistance to powdery mildew (Microsphaera diffusa)         in Brazilian soybean populations. Genet Mol Biol 25:339–342
    9. Hawtin G, Iwanaga M, Hodgkin T (1996) Genetic resources in breeding for adaptation. Euphytica 92:255–266
    10. He R, Chang Z, Yang Z, Yuan Z, Zhan H, Zhang X, Liu J (2009) Inheritance and mapping of powdery mildew resistance         gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180
    11. Hua W, Liu Z, Zhu J, Xie C, Yang T, Zhou Y, Duan X, Sun Q (2009) Identification and genetic mapping of pm42, a new         recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides).         Theor Appl Genet 119:223–230
    12. Humphry ME, Magner T, McIntyre CL, Aitken EA, Liu CL (2003) Identification of major locus conferring resistance to         powdery mildew (Erysiphe polygoni D.C.) in mungbean (Vigna radiata L. Wiczek) by QTL analysis. Genome         46:738–744
    13. Jun TH, Rouf Mian MA, Michel AP (2012) Genetic mapping revealed two loci for soybean aphid resistance in PI             567301B. Theor Appl Genet 124:13–22
    14. Kang ST, Mian MA (2010) Genetic map of the powdery mildew resistance gene in soybean PI 243540. Genome 53:400–405
    15. Kasettranan W, Somta P, Srinives P (2010) Mapping of quantitative trait loci controlling powdery mildew resistance in         mungbean (Vigna radiata L. Wilczek). J Crop Sci Biotechnol 13(3):155–161
    16. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
    17. Li CY, Wei J, Lin YJ, Chen H (2012) Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new         paradigm in plant breeding. Plant Cell Rep 31:851–862
    18. Li G, Fang T, Zhang H, Xie C, Li H, Yang T, Nevo E, Fahima T, Sun Q, Liu Z (2009) Molecular identification of a new         powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var.         dicoccoides). Theor Appl Genet 119:531–539
    19. Li ZZ, Sanchez A, Angeles E, Singh S, Domingo J, Huang N, Khush GS (2001) Are the dominant and recessive plant         disease resistance genes similar?: A case study of rice R genes and Xanthomonas oryzae pv. oryzae races.         Genetics 159:757–765
    20. Lillemo M, Asalf B, Singh R, Huerta-Espino J, Chen X, He Z, Bjørnstad Å (2008) The adult plant rust resistance loci         Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat         line Saar. Theor Appl Genet 116:1155–1166
    21. Lin Mction , Rios GP (1985) Cowpea diseases and their prevalence in Latin America. Pages 199-204 in Cowpea             research, production and utilization, edited by Singh SR and Rachie KO, John Wiley and Sons, Chichester, UK
    22. Lohnes DG, Bernard RL (1992) Inheritance of resistance to powdery mildew in soybeans. Plant Dis 76:964–965
    23. Luo PG, Luo HY, Chang ZJ, Zhang HY, Zhang M, Ren ZL (2009) Characterization and chromosomal location of Pm40         in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor         Appl Genet 118:1059–1064
    24. Maguire T, Collins G, Sedgley M (1994) A modified CTAB DNA extraction procedure for plants belonging to the family         proteaceae. Plant Mol Biol Rep 12: 106–109
    25. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene         symbols for wheat: 2008 supplement. Annu Wheat Newsl 54:209–225. http://wheat.pw.usda.gov/GG2/pubs.shtml
    26. Mignucci JS, Lim SM (1980) Powdery mildew (Microspraera diffusa) development on soybeans with adult-plant resistance.         Phytopathology 70:919–921
    27. Muchero M, Diop NN, Bhat PR, Fenton RD, Wanamaker S, Pottorff M, Hearne S, Cisse N, Fatokun C, Ehlers JD,         Roberts PA, Close TJ  (2009) A consensus genetic map of cowpea [Vigna unguiculata L. Walp] and synteny         based on EST-derived SNPs. Proc Natl Acad Sci USA 106: 18159–18164
    28. Raju SG, Anilkumar TB (1990) Evaluation of cowpea genotypes for partial resistance to powdery mildew. Euphytica         50:191–195
    29. Raju SG, Anilkumar TB (1991) Sources of partial resistance to powdery mildew in cowpea. Archiv für Phytopathologie         and Pflanzenschutz 27:153–156
    30. Smart J (1976) Tropical pulses. Longman Group Ltd, London
    31. Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale         genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective         against multiple pathogens in wheat. Theor Appl Genet 116:481–490
    32. Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS         conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet             126:1397–1403
    33. Xu P, Wu XH, Wang BG, Liu RH, Ehlers JD, Close TJ, Roberts PA, Diop NN, Qin DH, Hu TT, Lu ZF, Li GJ (2011) A         SNP and SSR based genetic map of Asparagus Bean (Vigna. unguiculata ssp. sesquipedialis) and comparison         with the broader species. PLoS ONE 6(1): e15952. doi:10.1371/journal.pone.0015952
    34. Young ND, Danesh D, Menancio-Hautea D, Kumar L (1993) Mapping oligogenic resistance to powdery mildew in             mungbean with RFLPs. Theor Appl Genet 87:243–249
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