Legume Research

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Legume Research, volume 37 issue 1 (february 2014) : 32-36

IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016

Xiaohua Wu, Baogen Wang, Zhongfu Lu, Xinyi Wu, Guojing Li, Pei Xu*
1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China
Cite article:- Wu Xiaohua, Wang Baogen, Lu Zhongfu, Wu Xinyi, Li Guojing, Xu* Pei (2024). IDENTIFICATION AND MAPPING OF A POWDERY MILDEW RESISTANCE GENE VU-PM1 IN THE CHINESE ASPARAGUS BEAN LANDRACE ZN016. Legume Research. 37(1): 32-36. doi: 10.5958/j.0976-0571.37.1.005.

ABSTRACT

Powdery mildew (PM), caused by the fungus Erysiphe polygoni D.C, is one of the most common and serious diseases of cowpea (Vigna. unguiculata L. Walp.)/asparagus bean (Vigna. unguiculata ssp. sesquipedialis). Deployment of scab-resistant varieties is the major strategy to curb this disease. ZN016, a Chinese asparagus bean landrace, is highly resistant to powdery mildew. To identify the resistance genes in ZN016, an F7:8 recombinant inbred line (RIL) population derived from the cross between ZN016 and a susceptible cultivar Zhijiang282 was evaluated for PM resistance in the open field in 2008 and 2009. Phenotypic analysis indicated that ZN016 harbors a single PM resistance gene. Using a SNP and SSR based genetic map constructed with this population, the PM resistance gene was mapped to a locus on LG9 flanked by SSR markers CLM0305 and CLM0260, with a genetic distance of 2.0 cM and 5.0 cM, respectively. To our knowledge, this is the first powdery mildew resistance gene reported in cowpea, which was temporarily designated as Vu-Pm1. The identification of Vu-Pm1 opens a door for dissecting resistance mechanisms and applying marker-assisted selection for the development of cultivars with PM resistance in cowpea.

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