Legume Research

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Legume Research, volume 37 issue 2 (april 2014) : 145-154

EXISTENCE OF ALTERNATE DEFENSE MECHANISMS FOR COMBATING MOISTURE STRESS IN HORSE GRAM [MACROTYLOMA UNIFLORUM (LAM.) VERDC.]

J.K. Yasin*, M.A. Nizar, 1 S. Rajkumar, M. Verma, N. Verma, S. Pandey, S.K. Tiwari2, J. Radhamani
1National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110 012, India
Cite article:- Yasin* J.K., Nizar M.A., Rajkumar S. 1, Verma M., Verma N., Pandey S., Tiwari2 S.K., Radhamani J. (2024). EXISTENCE OF ALTERNATE DEFENSE MECHANISMS FOR COMBATING MOISTURE STRESS IN HORSE GRAM [MACROTYLOMA UNIFLORUM (LAM.) VERDC.]. Legume Research. 37(2): 145-154. doi: 10.5958/j.0976-0571.37.2.022.

ABSTRACT

Screening of horse gram (Macrotyloma uniflorum) core collection was carried out under moisture stress conditions in glass house and in poly ethylene glycol 8000 solutions with an osmotic potential of -0.15 MPa to -1.76 MPa after germination. However, selected 50 accessions from the above study were raised under controlled moisture stress conditions to analyze variations in the magnitude of different enzyme activities among the surviving accessions. RWC remained unaltered during moisture stress condition but alteration in the plant structure was observed. Catalase activity and superoxide dismutase increased during moisture stress condition, which varied among the contrasting accessions. However, reduction in polyphenol oxidase and ascorbic acid oxidase activity was observed. The observed difference in anatomy confirms the presence of mechanisms other than antioxidant enzymes. Based on the performance in varying moisture stress conditions, the contrasting D9 and D14 were selected for breeding programme while D13 is recommended as a suitable cultivar under both irrigated and rainfed conditions. Differential enzyme activity, reduction in total sugar production and structural compaction were observed as mechanisms of energy conservation in horse gram to resist moisture stress conditions.

REFERENCES

  1. Abedi, T, and Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) Czech J. Genet. Plant Breed. 46: 27-34.
  2. Aebi, H. (1984). Catalase in vitro. Methods Enzymol. 105: 121-126.
  3. Amirjani, M.R. (2010). Effect of salinity stress on growth, mineral composition, proline content, antioxidant enzymes of soybean. Am. J. Plant Physiol. 5: 350-360.
  4. Aono, M. Saji, H., Sakamoto, A., Tanaka, K., Kondo, N and Tanaka, K. (1995). Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase. Plant Cell Physiol. 36: 1687-1691.
  5. Arora, R.K, and Chandel, K.P.S. (1972). Botanical source areas of wild herbage legumes in India. Trop. Grasslands 6: 213-221.
  6. Barrs, H.D and Weatherley, P.E. (1962). A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust. J. Biol. Sci. 15: 413-428.
  7. Blumenthal, M.J and Staples, I.B. (1993). Origin, evaluation and use of Macrotyloma as forage – a review. Trop. Grasslands 27: 16-29.
  8. Bravo, L. Siddhuraju, P and Saura-Calixto, F. (1999). Composition of underexploited Indian pulses. Comparison with common legumes. Food Chem. 64: 185-192.
  9. Castillo, F.I., Penel, I and Greppin, H. (1984). Peroxidase release induced by ozone in Sedum album leaves.Plant Physiol. 74: 846-851.
  10. Chen, W. Provart NJ, Glazebrook J, Katagiri F, Chang HS, Eulgem T, Mauch F, Luan S, Zou G, et al. (2002). Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. Plant Cell 14: 559-574.
  11. Dhindsa, R.A., Plumb-Dhindsa, P and Thorpe, T.A. (1981). Leaf senescence: Correlated with increased permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Bot. 126: 93-101.
  12. Dhindsa, R.S and Cleland, R.E. (1975). Water stress and protein synthesis. I. Differential inhibition of protein synthesis. Plant Physiol. 55: 778-781.
  13. Enstone, D.E., Peterson, C.A, and Ma F,S. (2003). Root endodermis and exodermis: structure, function, and responses to the environment. J. Plant Growth Regul. 21: 335-351.
  14. Gazanchian, A., Hajheidari, M., Sima, N.K., Salekdeh, G.H. (2007). Proteome response of Elymus elongatum to severe water stress and recovery.J. Exp. Bot. 58: 291-300.
  15. Grafi, G., Caspi, V.C., Nagar, T., Plaschkes, I., Barak, S and Ransbotyn, V. (2011). Plant response to stress meets dedifferentiation. Planta 233: 433-438.
  16. Knight, H, and Knight, M.R. (2001). Abiotic stress signalling pathways: specificity and cross-talk. Trends in Plant Sci. 6: 262-267.
  17. Li, L, and Steffens J.C. (2002). Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 215: 239-247.
  18. Logan, B.A., Kornyeyev, D., Hardison, J., Holaday, A.S. (2006). The role of antioxidant enzymes in photoprotection. Photosynthesis Res 88: 119-132.
  19. Michel, B.E. and Kaufmann, M.R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914.
  20. Monteiro, C.C., Carvalho, R.F., Gratao, P.L., Carvalho, G., Tezoto, T., Medici, L.O., Peres, L.E.P and Azevedo, R.A. (2011). Biochemical responses of the ethylene-insensitive Never ripe tomato mutant subjected to cadmium and sodium stresses. Environ. Exp. Bot. 71: 306-320.
  21. Nakayama, T. Yonekura-Sakakibara K, Sato, T., Kikuchi, S., Fukui, Y., Fukuchi-Mizutani, M., Ueda, T., Nakao, M., Tanaka, Y., Kusumi, T and Nishino, T. (2000). Aureusidin synthase: a polyphenol oxidase homolog responsible for flower coloration. Science 290: 1163-1166.
  22. Palatnik, J.F., Carrillo, N and Valle, E. M. (1999). The role of photosynthetic electron transport in the oxidative degradation of chloroplastic glutamine synthetase. Plant Physiol. 121: 471-478.
  23. Peshin, A. and Singla, S.K. (1994). Anticalcifying properties of Dolichos biflorus (horse gram) seeds. Indian J. Exp. Biol. 32: 889-91.
  24. Reinhardt, D.H. and Rost, T.L. (1995). Salinity accelerates endodermal development and induces an exodermis in cotton seedling roots. Environ. Exp. Bot. 35: 563-574.
  25. Sairam, R.K. and Tyagi, A. (2004). Physiology and molecular biology of salinity stress tolerance in plants.Curr. Sci. 86: 407-421.
  26. Sarvesh, A and Reddy, T. (1988). Peroxidase, polyphenol oxidase, acid phosphatase and alkaline inorganic pyrophosphatase activities during leaf senescence in varieties of castor (Ricinus communis L.), Indian J. Expl Biol. 26: 133-136.
  27. SAS institute (2009). Version 9.2. SAS institute, carry, North Carolina, USA.
  28. Steven, E. and Ruzin (1999). Plant micro-techniques and microscopy, Oxford University Press.oxford.
  29. Szucs, A. Jager, K., Jurca, M. E., Fabian, A., Bottka, S., Zvara, A., Barnabas, B and Feher, A. (2010). Histological and microarray analysis of the direct effect of water shortage alone or combined with heat on early grain development in wheat (Triticum aestivum). Physiol. Plant. 140: 174-188.
  30. Tait M.A. and Hik, (2003). Is dimethyl sulfoxide a reliable solvent for extracting chlorophyll under field conditions? Photosynthesis Res 78: 87-91.
  31. Thipyapong, P. Melkonian, J., David, W.W. and Steffens, J. C. (2004). Suppression of polyphenol oxidases increases stress tolerance in tomato. Plant Sci. 167: 693-703.
  32. Vines, H.M and Oberbacher, M.F. (1963). Citrus Fruit Enzymes. I. Ascorbic Acid Oxidase in Oranges.Plant Physiol. 38: 333-337.
  33. Wahid, A. GelaniS., Ashraf, M and Foolad, M.R. (2007). Heat tolerance in plants: An overview. Environ. Exp. Bot. 61: 199-223.
  34. Xu, C. and Huang, B. (2008). Root proteomic responses to heat stress in two Agrostis grass species contrasting in heat tolerance. J. Exp. Bot. 59: 4183-4194.
  35. Yasin, J.K., Bhat, K.V., Singh, N., Pandey, S., Pandey, C., Nizar, M.A., Verma, N., Negi, K.S., Tomar, J.B. and Asha, K.I. (2012). Diversity analysis of horse gram based on geographical distribution and quantitative traits. National Seminar on “Plant Genetic Research for Eastern and North-eastern India”
  36. Yasin, J.K., Bhat, K.V., Rajkumar, S., Subhalakshmi, A., Pillai, M.A., Fiyaz, A.R. and Ramya, K.T. (2013). Structural compaction: Mechanism of acid tolerance in moisture stress responsive horse gram accession. In: Proceedings of “The 8th International symposium on plant-soil interactions at low pH. Page 170-171.
  37. Yemm, E.W. and Willis, A.J. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochem. J. 57: 508-514.
  38. Yu, X., Peng Y.H., Zhang M.H., Shao Y.J., Su W.A. and Tang Z.C. (2006). Water relations and an expression analysis of plasma membrane intrinsic proteins in sensitive and tolerant rice during chilling and recovery. Cell Res. 16: 599-608.
  39. Zhang, J and Kirkham, M.B. (1994). Drought-Stress-Induced Changes in Activities of Superoxide Dismutase, Catalase, and Peroxidase in Wheat Species. Plant Cell Physiol. 35 : 785-791.
  40. Zhang, J and Kirkham, M.B. (1996). Antioxidant responses to drought in sunflower and sorghum seedlings New Phytologist 132: 361-373.
  41. Zhang, J.H., Huang,W.D., Liu, Y.P and Pan, Q.H. (2005). Effects of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L. cv. Jingxiu) under cross-temperature stresses. J. Integr. Plant Biol. 47: 959-970.
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