MAXIMIZATION OF MUTATION FREQUENCY IN GRASSPEA (LATHYRUS SATIVUS L.)

Article Id: ARCC885 | Page : 296 - 299
Citation :- MAXIMIZATION OF MUTATION FREQUENCY IN GRASSPEA (LATHYRUS SATIVUS L.).Legume Research-An International Journal.2011.(34):296 - 299
S.K. Tripathy, D. Lenka and Rajesh Ranjan
Address : Orissa University of Agricultural Technology, College of Agriculture, Bhubaneswar - 751 003, India

Abstract

Frequency of mutations (Chlorophyll, foliar and agronomic) have been reported following seed treatment of grasspea cv. Dhenkanal local with - rays, EMS, NG and their combinations at varying doses. NG was the most potent mutagen for induction of mutation followed by EMS and -rays . Combination treatments showed synergistic effect for mutation frequency. EMS and NG, singly or in combination with -rays, were more or less equipotent in inducing agronomic mutations, while,  - ray was less effective.

Keywords

Mutation frequency - rays Ethyl methanesulphonate (EMS) N-ethyl-N-nitro-N-nitrosoguanidine (NG) Lathyrus sativus.

References

  1. Dellaret, L.M.W. (1980). Dose response curves after irradiation of Arbidopsis seeds : A possible explanation for the saturation in mutation frequency at high radiation doses. Environ Exp. Bot 20 : 309-323.
  2. Ehrenberg, L. and Nybom, N. (1954). Ion density and biological effectiveness of radiations. Acta Agric Scand 4 : 396-418.
  3. Ehrenberg, L. Lundqvist, U. and Ahnstrom, G. (1958). The mutagenic action of ethylene imine in barley. Hereditas 44 : 330-336.
  4. Filippetti, A. and Pace, C.De. (1986). Improvement of seed yield in Vicia faba L. by using experimental mutagenesis : II. Comparison of gamma-radiation and ethyl methanesulphonate (EMS) in production of morphological mutants. Euphytica 35 : 49-59.
  5. Haque, M.Z. and Godward MBE (1986). Plastid ultrastructure in chlorophyll mutant sectors of leaves in seed irradiated Lectuceae. Cytologia 51: 777-784.
  6. Keiding Niels, (1970). The variance of X2 mutant frequency estimate in barley. Hereditas 66 : 263-268.
  7. Sharma, S.K. and Sharma, B. ( 1986). Mutagen sensitivity and mutability in lentil. Theor Appl Genet 71: 820-825 .
  8. Singh, R.M. Singh, J. and Srivastava, A.N. (1977). Mutagenic effects of gamma-rays, EMS and HA in barley. Barley Genet Newsletter 7 : 60.
  9. Swaminathan, M.S. (1957). Swedish mutation work in relation to plant breeding- A review. Special symposium on Genetics and Plant Breeding in South Asia. Indian J Genet and Pl Breed 17 (2) : 176-295.
  10. Tripathy, S.K. (1997). Genetic rectification in Khesari (Lathyrus sativus L.). Ph. D. Thesis, Utkal Univ., Vani Vihar, Bhubaneswar (Orissa), India,p. 35-60.
  11. Von Wettstein, D. (1980). Origin of chloroplasts. (Schiff JA and Stainer RY, eds) Elsevier, North Holland. p. .65.
  12. Vaz Patto, M.C., Skiba, B. Pang E.C.K., Ochatt, S.J., Lambein F and Rubiales, D. (2006). Evaluation of selected mutants of grasspea (Lathyrus sativus L.). Euphytica, 147 (1-2): 133-147.

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