Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 34 issue 4 (december 2011) : 242 - 250

TOXICOLOGICAL SCREENING OF SOME SELECTED LEGUMES SEED EXTRACTS

M
M. Zia-ul-haq1
M
M. Nisar2
M
M.R. Shah3
M
M. Akhter4
M
M. Qayum5
S
S. Ahmad6
S
Shakir A. Shahid7
M
Mirza Hasanuzzaman8*
1Department of Pharmacognosy University of Karachi, Karachi - 752 70, Pakistan

  • Submitted|

  • First Online |

  • doi

Cite article:- Zia-ul-haq1 M., Nisar2 M., Shah3 M.R., Akhter4 M., Qayum5 M., Ahmad6 S., Shahid7 A. Shakir, Hasanuzzaman8* Mirza (2025). TOXICOLOGICAL SCREENING OF SOME SELECTED LEGUMES SEED EXTRACTS. Legume Research. 34(4): 242 - 250. doi: .

ABSTRACT

Legumes are excellent sources of protein, carbohydrates, dietary fibre, lipids, vitamins, carotenoids, macronutrients, a variety of micronutrients and phytochemicals. In a study carried in the University of Karachi in 2009, ethanolic extracts of seeds of eight legumes namely pea (Pisum sativum), desi and kabuli chickpea (Cicer arietinum L), cowpea (Vigna unguiculata (L) Walp.), mash bean (Phaseolus mungo), mung bean (Vigna radiate L. wilczek), soybean (Glycine max) and lentil (Lens culinaris Medik.) were tested for different biological activities such as insecticidal activity, phytotoxic activity, wormicidal, anthelmintic activity as well as lethality bioassay. Most extracts (desi chickpea, cowpea, mash bean, mung bean, soybean and lentil) showed moderate activity at higher concentration of 1000 ìg ml-1 and low or no activity at low doses (100 and 10µg ml-1). In vitro results showed that ethanolic extract of these plants caused appreciable mortality of second stage juveniles of Cephalobus litoralis and Helicotylenchus indicus. Higher concentrations used (2 % and 1 %) were found more effective and produced significant results as compared to 0.5 % of extracts. The mortality rate increased with increasing exposure time and was highest after 48 hours.

REFERENCES

  1. Ahmad, S., Akhter, M., Zia-ul-Haq, M., Mehjabeen and Ahmed, S. (2010) Antifungal and nematicidal activity of selected legumes of Pakistan. Pak. J. Bot. 42: 1327–1331.
  2. Ali, I., Rubina, N., Wahib, N.K., Rukhsana, G. and Choudhary, M.I. (2009) Biological screening of different root extracts of Euphorbia wallichii. Pak. J. Bot. 41: 1737–1741.
  3. Aswar, M., Urmila, A., Bhagyashri, W., Vyas, M., Wagh, A. and Gujar N. K. (2008) Anthelmintic activity of Ficus benghalensis. Indian J. Green Pharm. 2:170-172.
  4. Baermann, G. (1917) Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden)-Larven in Erdproben Welteureden Batavia Geneesk. Tijschr. Ned. Ind. 57: 131–137.
  5. Bate-Smith, E.C. (1962) The phenolic constituent of plants and their taxonomic significance, dicotyledons. J. Linn. Soc. Bot. 58: 95–103.
  6. Caryrol, J.C., Djian, C. and Pijarowski, I. (1989) Studies on the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Rev. Nematol. 12: 331–336.
  7. Cobb, N.A. (1918) Estimating the nema population of soil. Agric Tech Circ U.S. Deptt. Agric. 1: 4.
  8. Cresswell, K. J. (2007) Anthelmintic effects of tropical shrub legumes in ruminant animals. PhD thesis, James Cook University.
  9. Deore, S.L, Khadabadi, S.S., Kamdi, K.S., Ingle, V.P., Kawalkar, N.G.S.P.S., Patil, U.A. and Vyas, A.J. (2009) In vitro anthelmintic activity of Cassia tora. Int. J. Chem. Tech. Res. 2:177–179.
  10. Girón-Calle, J., Vioquem, J., Mar, M., Pedroche, J., Alaizm, M. and Millán F. (2004) Effect of chickpea aqueous extracts, organic extracts, and protein concentrates on cell proliferation. J. Medicinal Food. 7: 122-129. 
  11. Jembere, B., Obeng-Ofori, D., Hassanali, A. and Nyamasyo, G.N.N. (1995) Products derived from the leaves of Ocimum kilimanndscharium (Labiatae) as post-harvest grain protectants against the infestation of three major stored product insect pests. Bull. Entomol. Res. 85:361-367.
  12. Jourand, P., Rapior, S., Fargette, M. and Mateille, T. (2004) Nematostatic activity of aqueous extracts of West African Crotalaria species. Nemat. 6: 765–771.
  13. Kan, A., Özçelik, B., Kartal, M., Özdemir, Z.A. and Özgen, S. (2010) In vitro antimicrobial activities of Cicer arietinum L. (Chickpea). Trop. J. Pharm. Res. 9: 475–481.
  14. Kan, A., Özçelik, B. and Kartal, M. (2009) In vitro antiviral activities under cytotoxic doses against Herpes simples type-1 and Parainfluensa-3 viruses of Cicer arietinum L. (Chickpea). Afr. J. Pharm. Pharmacol. 3: 627–631.
  15. Khurma, U. R. and Mangotra, A. (2004) Screening of some Leguminosae seeds for nematicidal activity. South Pacific J. Nat. Sci. 22: 51–53.
  16. Matthews, G. A. (1993) Insecticide application in the stores. In Matthews, G.A. and Hislop, E.C. (eds.). Application technology for crop protection. CAB, London, pp. 305-315
  17. Meyer, N.B., Ferrigni, R.N., Putnam, E.J., Jacobsen, B.L., Nichols, E.D. and Mclaughlin, I.J. (1982) Brine shrimp: A convenient general bioassay for active plant constituents. J. Planta Medica 45: 31–35.
  18. Morris, J. B. and Walker, J.T. (2002) Non-traditional legumes as potential soil amendments for nematode control. J. Nemat. 34: 358–361.
  19. Mukherjeem, S. N. and Joseph, M. (2000) Medicinal plant extracts influencing insect growth and reproduction. J. Med. Arom. Plant Sci. 22: 38-39.
  20. Naqvi, B. S., Khan, A., Shaikh, D. and Shaikh, M. R. (1992) Nematicidal properties of selected marine algae from Karachi coast. J. Islamic Acad Sci. 5: 171–172.
  21. Nazli, R., Akhter, M., Ambreen, S., Solangi, A.H. and Sultana, N. (2008) Insecticidal, nematicidal and antibacterial activities of Gliricidia sepium. Pak. J. Bot. 40: 2625–2629.
  22. Nisar, M., Qayum, M., Shah, M. R., Kaleem, W.A., Ali, I. and Zia-ul-Haq, M. (2010) Antimicrobial screening of Impatiens bicolor Royle. Pak. J. Bot. 42: 523–526.
  23. Nisar, M., Qayum, M., Shah, M.R., Kaleem, W.A., Ali, I. and Zia-ul-Haq, M. (2010b) Biological screening of Impatiens bicolor royle. Pak. J. Bot. 42: 523–526.
  24. Nisar, M., Kaleem, W.A., Qayum, M., Hussain, A., Zia-ul-Haq, M., Ali, I. and Choudhary, M. I. (2010c) Biological screening of Zizyphus oxyphylla Edgew leaves. Pak. J. Bot. 42: 4063–4069.
  25. nisar, M., Kaleem, W. A., qayum, M., Marwat, I. K., Zia-Ul-Haq, M., Ali, I. and Choudhary, M. I. (2011) Biological screening of Zizyphus oxyphylla edgew stem. Pak. J. Bot., 43: 311-317.
  26. Qamar, F., Saeed, M., Kapadia, Z., Seema, N. and Badar, Y. (1989) Nematicidal properties of crude extracts of some indigenous plants.Part 1. Pak. J. Sci. Ind. Res. 32: 600–602.
  27. Rashid, R., Farah, M. and Mirza, M. N. (2009) Biological screening of Salvia cabulica. Pak. J. Bot. 41: 1453–1462.
  28. Shanmugasundaram, S. (2000) Processing and utilization of legumes. Asian Productivity Organization, Hirakawacho, Chiyoda-ku, Tokyo.
  29. Su, H.F.C. (1990) Biological activities of hexane extract of Piper cubeba against rice weevils and cowpea weevils (Coleoptera: Curculionidae). J. Entomol. Sci. 25:16-20.
  30. Waterman, P. G. and Mole, S. (1994) Analysis of Phenolic Plant Metabolites, Oxford University Publications, Oxford.
  31. Zettler, J. L. and Cuperus, G. W. (1990) Pesticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) in wheat. J. Econ. Entomol. 83: 1677-1681.
  32. Zia-ul-Haq, M., Iqbal, S., Ahmad, S., Imran, M., Niaz, A. and Bhanger, M. I. (2007a) Nutritional and compositional study of desi chickpea (Cicer arietinum L.) cultivars grown in Punjab, Pakistan. Food Chem. 105:1357–1363.
  33. Zia-ul-Haq, M., Ahmad, M., Iqbal, S., Ahmad, S. and Ali, H. (2007b) Characterization and compositional studies of oil from seeds of desi chickpea (Cicer arietinum L.) cultivars grown in Pakistan. J. Am. Oil. Chem. Soc. 84:1143–1148.
  34. Zia-ul-Haq, M., Iqbal, S. and Ahmad, M. (2008a) Characteristics of oil from seeds of 4 mungbean (Vigna radiate L. wilczek) cultivars grown in Pakistan. J. Am. Oil Chem. Soc. 85: 851–856.
  35. Zia-ul-Haq, M., Iqbal, S., Ahmad, S., Bhanger, M. I., Wiczkowski, W. and Amarowicz, R. (2008b) Antioxidant potential of desi chickpea varieties commonly consumed in Pakistan. J. Food Lipid 15: 26-342.
  36. Zia-ul-Haq, M., Ahmad, S., Ahmad, M., Iqbal, S. and Khawar, K.M. (2009) Effects of cultivar and row spacing on tocopherol and sterol composition of chickpea (Cicer arietinum L) seed oil. Tarim Bilimleri Dergisi 15: 25–30.
  37. Zia-ul-Haq, M., Ahmad, S., Chiavaro, E., Mehjabeen and Ahmed, S. (2010) Studies of oil from cowpea (Vigna unguiculata (L.) walp.) cultivars commonly grown in Pakistan. Pak. J. Bot. 42: 214–220.
  38. Zia-ul-Haq, M., Ahmad, S., Shad, M. A., Iqbal, S., Qayum, M., Ahmad, A., Luthria, D. L. and Amarowicz, R. (2011) Compositional studies of some of lentil cultivars commonly consumed in Pakistan. Pak. J. Bot., 43: 1563-1567.
Published In
Legume Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 34 issue 4 (december 2011) : 242 - 250

    TOXICOLOGICAL SCREENING OF SOME SELECTED LEGUMES SEED EXTRACTS

    M
    M. Zia-ul-haq1
    M
    M. Nisar2
    M
    M.R. Shah3
    M
    M. Akhter4
    M
    M. Qayum5
    S
    S. Ahmad6
    S
    Shakir A. Shahid7
    M
    Mirza Hasanuzzaman8*
    1Department of Pharmacognosy University of Karachi, Karachi - 752 70, Pakistan

    • Submitted|

    • First Online |

    • doi

    Cite article:- Zia-ul-haq1 M., Nisar2 M., Shah3 M.R., Akhter4 M., Qayum5 M., Ahmad6 S., Shahid7 A. Shakir, Hasanuzzaman8* Mirza (2025). TOXICOLOGICAL SCREENING OF SOME SELECTED LEGUMES SEED EXTRACTS. Legume Research. 34(4): 242 - 250. doi: .

    ABSTRACT

    Legumes are excellent sources of protein, carbohydrates, dietary fibre, lipids, vitamins, carotenoids, macronutrients, a variety of micronutrients and phytochemicals. In a study carried in the University of Karachi in 2009, ethanolic extracts of seeds of eight legumes namely pea (Pisum sativum), desi and kabuli chickpea (Cicer arietinum L), cowpea (Vigna unguiculata (L) Walp.), mash bean (Phaseolus mungo), mung bean (Vigna radiate L. wilczek), soybean (Glycine max) and lentil (Lens culinaris Medik.) were tested for different biological activities such as insecticidal activity, phytotoxic activity, wormicidal, anthelmintic activity as well as lethality bioassay. Most extracts (desi chickpea, cowpea, mash bean, mung bean, soybean and lentil) showed moderate activity at higher concentration of 1000 ìg ml-1 and low or no activity at low doses (100 and 10µg ml-1). In vitro results showed that ethanolic extract of these plants caused appreciable mortality of second stage juveniles of Cephalobus litoralis and Helicotylenchus indicus. Higher concentrations used (2 % and 1 %) were found more effective and produced significant results as compared to 0.5 % of extracts. The mortality rate increased with increasing exposure time and was highest after 48 hours.

    REFERENCES

    1. Ahmad, S., Akhter, M., Zia-ul-Haq, M., Mehjabeen and Ahmed, S. (2010) Antifungal and nematicidal activity of selected legumes of Pakistan. Pak. J. Bot. 42: 1327–1331.
    2. Ali, I., Rubina, N., Wahib, N.K., Rukhsana, G. and Choudhary, M.I. (2009) Biological screening of different root extracts of Euphorbia wallichii. Pak. J. Bot. 41: 1737–1741.
    3. Aswar, M., Urmila, A., Bhagyashri, W., Vyas, M., Wagh, A. and Gujar N. K. (2008) Anthelmintic activity of Ficus benghalensis. Indian J. Green Pharm. 2:170-172.
    4. Baermann, G. (1917) Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden)-Larven in Erdproben Welteureden Batavia Geneesk. Tijschr. Ned. Ind. 57: 131–137.
    5. Bate-Smith, E.C. (1962) The phenolic constituent of plants and their taxonomic significance, dicotyledons. J. Linn. Soc. Bot. 58: 95–103.
    6. Caryrol, J.C., Djian, C. and Pijarowski, I. (1989) Studies on the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Rev. Nematol. 12: 331–336.
    7. Cobb, N.A. (1918) Estimating the nema population of soil. Agric Tech Circ U.S. Deptt. Agric. 1: 4.
    8. Cresswell, K. J. (2007) Anthelmintic effects of tropical shrub legumes in ruminant animals. PhD thesis, James Cook University.
    9. Deore, S.L, Khadabadi, S.S., Kamdi, K.S., Ingle, V.P., Kawalkar, N.G.S.P.S., Patil, U.A. and Vyas, A.J. (2009) In vitro anthelmintic activity of Cassia tora. Int. J. Chem. Tech. Res. 2:177–179.
    10. Girón-Calle, J., Vioquem, J., Mar, M., Pedroche, J., Alaizm, M. and Millán F. (2004) Effect of chickpea aqueous extracts, organic extracts, and protein concentrates on cell proliferation. J. Medicinal Food. 7: 122-129. 
    11. Jembere, B., Obeng-Ofori, D., Hassanali, A. and Nyamasyo, G.N.N. (1995) Products derived from the leaves of Ocimum kilimanndscharium (Labiatae) as post-harvest grain protectants against the infestation of three major stored product insect pests. Bull. Entomol. Res. 85:361-367.
    12. Jourand, P., Rapior, S., Fargette, M. and Mateille, T. (2004) Nematostatic activity of aqueous extracts of West African Crotalaria species. Nemat. 6: 765–771.
    13. Kan, A., Özçelik, B., Kartal, M., Özdemir, Z.A. and Özgen, S. (2010) In vitro antimicrobial activities of Cicer arietinum L. (Chickpea). Trop. J. Pharm. Res. 9: 475–481.
    14. Kan, A., Özçelik, B. and Kartal, M. (2009) In vitro antiviral activities under cytotoxic doses against Herpes simples type-1 and Parainfluensa-3 viruses of Cicer arietinum L. (Chickpea). Afr. J. Pharm. Pharmacol. 3: 627–631.
    15. Khurma, U. R. and Mangotra, A. (2004) Screening of some Leguminosae seeds for nematicidal activity. South Pacific J. Nat. Sci. 22: 51–53.
    16. Matthews, G. A. (1993) Insecticide application in the stores. In Matthews, G.A. and Hislop, E.C. (eds.). Application technology for crop protection. CAB, London, pp. 305-315
    17. Meyer, N.B., Ferrigni, R.N., Putnam, E.J., Jacobsen, B.L., Nichols, E.D. and Mclaughlin, I.J. (1982) Brine shrimp: A convenient general bioassay for active plant constituents. J. Planta Medica 45: 31–35.
    18. Morris, J. B. and Walker, J.T. (2002) Non-traditional legumes as potential soil amendments for nematode control. J. Nemat. 34: 358–361.
    19. Mukherjeem, S. N. and Joseph, M. (2000) Medicinal plant extracts influencing insect growth and reproduction. J. Med. Arom. Plant Sci. 22: 38-39.
    20. Naqvi, B. S., Khan, A., Shaikh, D. and Shaikh, M. R. (1992) Nematicidal properties of selected marine algae from Karachi coast. J. Islamic Acad Sci. 5: 171–172.
    21. Nazli, R., Akhter, M., Ambreen, S., Solangi, A.H. and Sultana, N. (2008) Insecticidal, nematicidal and antibacterial activities of Gliricidia sepium. Pak. J. Bot. 40: 2625–2629.
    22. Nisar, M., Qayum, M., Shah, M. R., Kaleem, W.A., Ali, I. and Zia-ul-Haq, M. (2010) Antimicrobial screening of Impatiens bicolor Royle. Pak. J. Bot. 42: 523–526.
    23. Nisar, M., Qayum, M., Shah, M.R., Kaleem, W.A., Ali, I. and Zia-ul-Haq, M. (2010b) Biological screening of Impatiens bicolor royle. Pak. J. Bot. 42: 523–526.
    24. Nisar, M., Kaleem, W.A., Qayum, M., Hussain, A., Zia-ul-Haq, M., Ali, I. and Choudhary, M. I. (2010c) Biological screening of Zizyphus oxyphylla Edgew leaves. Pak. J. Bot. 42: 4063–4069.
    25. nisar, M., Kaleem, W. A., qayum, M., Marwat, I. K., Zia-Ul-Haq, M., Ali, I. and Choudhary, M. I. (2011) Biological screening of Zizyphus oxyphylla edgew stem. Pak. J. Bot., 43: 311-317.
    26. Qamar, F., Saeed, M., Kapadia, Z., Seema, N. and Badar, Y. (1989) Nematicidal properties of crude extracts of some indigenous plants.Part 1. Pak. J. Sci. Ind. Res. 32: 600–602.
    27. Rashid, R., Farah, M. and Mirza, M. N. (2009) Biological screening of Salvia cabulica. Pak. J. Bot. 41: 1453–1462.
    28. Shanmugasundaram, S. (2000) Processing and utilization of legumes. Asian Productivity Organization, Hirakawacho, Chiyoda-ku, Tokyo.
    29. Su, H.F.C. (1990) Biological activities of hexane extract of Piper cubeba against rice weevils and cowpea weevils (Coleoptera: Curculionidae). J. Entomol. Sci. 25:16-20.
    30. Waterman, P. G. and Mole, S. (1994) Analysis of Phenolic Plant Metabolites, Oxford University Publications, Oxford.
    31. Zettler, J. L. and Cuperus, G. W. (1990) Pesticide resistance in Tribolium castaneum (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Coleoptera: Bostrichidae) in wheat. J. Econ. Entomol. 83: 1677-1681.
    32. Zia-ul-Haq, M., Iqbal, S., Ahmad, S., Imran, M., Niaz, A. and Bhanger, M. I. (2007a) Nutritional and compositional study of desi chickpea (Cicer arietinum L.) cultivars grown in Punjab, Pakistan. Food Chem. 105:1357–1363.
    33. Zia-ul-Haq, M., Ahmad, M., Iqbal, S., Ahmad, S. and Ali, H. (2007b) Characterization and compositional studies of oil from seeds of desi chickpea (Cicer arietinum L.) cultivars grown in Pakistan. J. Am. Oil. Chem. Soc. 84:1143–1148.
    34. Zia-ul-Haq, M., Iqbal, S. and Ahmad, M. (2008a) Characteristics of oil from seeds of 4 mungbean (Vigna radiate L. wilczek) cultivars grown in Pakistan. J. Am. Oil Chem. Soc. 85: 851–856.
    35. Zia-ul-Haq, M., Iqbal, S., Ahmad, S., Bhanger, M. I., Wiczkowski, W. and Amarowicz, R. (2008b) Antioxidant potential of desi chickpea varieties commonly consumed in Pakistan. J. Food Lipid 15: 26-342.
    36. Zia-ul-Haq, M., Ahmad, S., Ahmad, M., Iqbal, S. and Khawar, K.M. (2009) Effects of cultivar and row spacing on tocopherol and sterol composition of chickpea (Cicer arietinum L) seed oil. Tarim Bilimleri Dergisi 15: 25–30.
    37. Zia-ul-Haq, M., Ahmad, S., Chiavaro, E., Mehjabeen and Ahmed, S. (2010) Studies of oil from cowpea (Vigna unguiculata (L.) walp.) cultivars commonly grown in Pakistan. Pak. J. Bot. 42: 214–220.
    38. Zia-ul-Haq, M., Ahmad, S., Shad, M. A., Iqbal, S., Qayum, M., Ahmad, A., Luthria, D. L. and Amarowicz, R. (2011) Compositional studies of some of lentil cultivars commonly consumed in Pakistan. Pak. J. Bot., 43: 1563-1567.
    In this Article
      Contact us
      Follow us
      Published In
      Legume Research

      Editorial Board

      View all (0)