Legume Research-An International Journal

DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM

Article Id: ARCC856 | Page : 129 - 133
Citation :- DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM.Legume Research-An International Journal.2011.(34):129 - 133
L. Prasanthi, B.V. Bhaskara Reddy, K. Rekha Rani, R. Mehala Devi, B. Geetha, Y. Siva Prasad and K.Raja Reddy
Address : Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.

Abstract

A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.

Keywords

Blackgram YMV RAPD SCAR marker.

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