Article Id: ARCC856 | Page : 129 - 133
Citation :- DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM.Legume Research-An International Journal.2011.(34):129 - 133
L. Prasanthi, B.V. Bhaskara Reddy, K. Rekha Rani, R. Mehala Devi, B. Geetha, Y. Siva Prasad and K.Raja Reddy
Address : Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.


A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.


Blackgram YMV RAPD SCAR marker.


  1. Gupta S., S.Kumar., R.A.Singh and S.Chandra 2005 Identification of a single dominant gene for resistance to mungbean yellow mosaic virus in blackgram (Vigna mungo(L.)Hepper ) SABRO J, 37(2): 85-89
  2. Melotto, M., Afanador L. and J.D.Kelly 1996. Development of a SCAR marker linked to the I gene in common bean. Genome, 39: 1212-1219
  3. Murray, M.G. and W.F.Thompson, 1986. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res, 8: 4321-4325
  4. Naqvi, N.I. and B.B. Chattoo, 1996. Development of SCAR based indirect selection method for dominant blast resistance gene in rice. Genome, 39: 26-30
  5. Singh,A.., A. Sirohi and K.S Panwar 1998 Inheritance of mungbean yellow mosaic virus resistance in urdbean (Vigna mungo).Indian J.Virol,14:89-90
  6. Souframanien.J. and T.Gopalakrishna 2006 ISSR and SCAR markers linked to the mungbean yellow mosaic virus resistance gene in blackgram (Vigna mungo (L.) Hepper )Plant Breeding, 125:619-622
  7. Sambrook.J FritschE.F and Maniatis T 1989"Molecular Cloning,a laboratory manual 2nd ed Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York,USA
  8. Verma R.P.S. and D.P.Singh 1989 Inheritance of Resistance to mungbean yellow mosaic virus in blackgram. Indian J.Genet., 49(3) :321-324
  9. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalsi J.A. and S.V. Tingey, 1990. DNA polymoephisms amplified by arbitary primres are useful as genetic markers. Nucleic Acid Res, 18: 6531-6535.
  10. Zhag. Y. and J.R. Stommet, 2001. Development of SCAR and CAPS markers linked to the Beta gene in tomato. Crop Sci., 41: 1602-1608.

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