volume 34 issue 2 (june 2011) : 129 - 133
DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM
1Regional Agricultural Research Station,
Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.
ABSTRACT
A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.
KEYWORDS
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This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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