volume 34 issue 2 (june 2011) : 129 - 133
DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM
1Regional Agricultural Research Station,
Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.
Published In
Legume Research
Article Metrics
0
Views
0
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
volume 34 issue 2 (june 2011) : 129 - 133
DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM
1Regional Agricultural Research Station,
Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.
ABSTRACT
A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.
KEYWORDS
REFERENCES
- Gupta S., S.Kumar., R.A.Singh and S.Chandra 2005 Identification of a single dominant gene for resistance to mungbean yellow mosaic virus in blackgram (Vigna mungo(L.)Hepper ) SABRO J, 37(2): 85-89
- Melotto, M., Afanador L. and J.D.Kelly 1996. Development of a SCAR marker linked to the I gene in common bean. Genome, 39: 1212-1219
- Murray, M.G. and W.F.Thompson, 1986. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res, 8: 4321-4325
- Naqvi, N.I. and B.B. Chattoo, 1996. Development of SCAR based indirect selection method for dominant blast resistance gene in rice. Genome, 39: 26-30
- Singh,A.., A. Sirohi and K.S Panwar 1998 Inheritance of mungbean yellow mosaic virus resistance in urdbean (Vigna mungo).Indian J.Virol,14:89-90
- Souframanien.J. and T.Gopalakrishna 2006 ISSR and SCAR markers linked to the mungbean yellow mosaic virus resistance gene in blackgram (Vigna mungo (L.) Hepper )Plant Breeding, 125:619-622
- Sambrook.J FritschE.F and Maniatis T 1989"Molecular Cloning,a laboratory manual 2nd ed Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York,USA
- Verma R.P.S. and D.P.Singh 1989 Inheritance of Resistance to mungbean yellow mosaic virus in blackgram. Indian J.Genet., 49(3) :321-324
- Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalsi J.A. and S.V. Tingey, 1990. DNA polymoephisms amplified by arbitary primres are useful as genetic markers. Nucleic Acid Res, 18: 6531-6535.
- Zhag. Y. and J.R. Stommet, 2001. Development of SCAR and CAPS markers linked to the Beta gene in tomato. Crop Sci., 41: 1602-1608.
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Published In
Legume Research