Legume Research

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Legume Research, volume 34 issue 2 (june 2011) : 129 - 133

DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM

L. Prasanthi, B.V. Bhaskara Reddy, K. Rekha Rani, R. Mehala Devi, B. Geetha, Y. Siva Prasad, K.Raja Reddy
1Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati - 517 502, India.

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Cite article:- Prasanthi L., Reddy Bhaskara B.V., Rani Rekha K., Devi Mehala R., Geetha B., Prasad Siva Y., Reddy K.Raja (2024). DEVELOPMENT OF RAPD/SCAR MARKER FOR YELLOW MOSAIC DISEASE RESISTANCE IN BLACKGRAM. Legume Research. 34(2): 129 - 133. doi: .

ABSTRACT

A total of 130 random oligonucleotide primers were surveyed to identify yellow mosaic resistant genotypes in blackgram. Primer OPQ-1525 was found to produce a consistent marker, which differentiated resistant genotype from susceptible genotype. An identified random amplified polymorphic DNA (RAPD) marker, OPQ-1, linked to YMV resistant was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region primer SCAR (20f/r). The marker designed was amplified at a specific site of 532bp only in resistant genotypes. This would help for screening different genotypes and segregating generations in marker-assisted breeding programme.

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