Rhizobial isolates of red sanders, Pterocarpus santalinus L. were fast growers and acid producers. The growth characteristics were determined on different media and they produced effective nodules on P. santalinus. Isolates showed good growth on different carbon sources including mono, di and polysaccharides, but moderate growth on sucrose, maltose and least on citrate. The isolates grew well with glutamic acid, aspergine, yeast extract and potassium nitrate as a source of nitrogen, but glycine was least preferred. Isolates grew well at a pH range of 5.0 to 8.0 but not below 4.0 and above 9.0 and they showed good growth at 0.1, 0.5 and 1.0 per cent of NaCl in the medium. All the rhizobial isolates of red sanders nodulalted Vigna radiata, Dalbergia sissoo and produced ineffective nodules on Leucaena leucocephala. The preliminary identification of the rhizobia of red sanders showed it to be similar to Rhizobium phaseoli. Cross infectivity of Rhizobium tested on Pterocarpus santalinus coincide exactly with that of DAC-ELISA results.