Legume Research, volume 29 issue 4 (december 2006) : 235 - 241

ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

C. Kole··, B. Mohanty, A. Pa12, Bithika Singh3, P.R. Kole4, Neeiam Shukla4

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¹Laboratory of Molecular Biology and Biotechnology, QUAT, Bhubaneswar - 751 003, India

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Cite article:- Kole·· C., Mohanty B., Pa12 A., Singh3 Bithika, Kole4 P.R., Shukla4 Neeiam (2025). ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Legume Research. 29(4): 235 - 241. doi: .

ABSTRACT

SDS-polyacrylamide gel electrophoresis of seed globulins of 24 blackgram genotypes displayed the presence of 12 distinctly scoreable polypeptide bands dispersed over four zones, A to D, with Rm values ranging fram 0.05 to 0.821. Five of these polypeptides (Rm: 0.05, 0.128, 0.171, 0.435 and 0.464) varied for their expression. A globulin profile was depicted for the first time based on the electrophoretic banding pattern for this crop. Classificatory analysis led to the identification of the two genptypes, AKU 7 and LBG 402 for their unique protein profiles. The dendrogram constructed revealed, in general, biochemical proximity of the genotypes developed at a particular location and sharing related pedigree. The results generated potentially useful information for future works aiming at elaborated biochemical delineation and their use in improving seed quality in blackgram.

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REFERENCES

Aykroyd, w.R. et al. (1982). Legumes In Human Nutrition, FAD, Rome, Italy.
Basha, S.M.M. (1979). Plant Physlol., 63: 301-306.
Chandana, M. et al. (1991). In: Proc. Golden Jubilee Celebrations Symp. Grain Legumes, New Deihl, 1991, Indian Soc. Genet. and Plant Breed, New Delhi, pp. 341-350.
Gepts, P. (1990). In: Plant Population Genetics, Breeding and Genetic Resources. (Brown, A.S.D. et a/., eds.). Sinauer, Sunderland MA; pp. 64-82.
Koeing, R.L. et aJ. (1990). Econ. Bot., 44: 50-60.
Kole, C. et aI. (2002). Indian J. Genet., 62: 345-346.
Kole, C. et aI. (2000). Crop Res., 20: 261-267.
Krishna, T.G. and Mitra, R. (1988). Euphytica, 37: 47-52.
Kumar, S. and Ram, H.H. (1989). Crop lmprov., 16: 5-8.
Ladlzinsky, G. and Adler, A. (1975). Israel J. Bot., 24: 183-189.
Laemmli, UK (1970). Nature, 227: 680-685.
Larse, AL. (1%7). Crop Sci., 7: 311-313.
Mohanty, J.B. et a/. (2001). Crop Res., 21: 134-138.
Nalk, B.S. and Kole, C. (2001). Legume Res., 24(3): 169-173.
Osborn, T.C. (1988). Genetic Control of Bean Seed Protein. CRC Critical Rev. Plant Sci., 7: 93-116.
Polignano, G.B. et aI. (1990). J. Genet. Breed., 44: 31-38.
Sahal, S. and Rana, R.S. (1977). New Phytol., 79: 527-534.
Schinkel, C. and Gepts, P. (1988). Plant Breed., 101: 292-301.
Sood, A. et a/. (1995). J. Plant Biotech., 4: 121-124.
Thakare, R.G. et a1. (1988). In: Proc. 2'ti Int. Symp. Mungbean. (Shanmugasundaram, S. and Mclean, B.T. eds.). AVRDC, Taiwan, pp. 47-52.

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ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

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