Legume Research

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Legume Research, volume 29 issue 4 (december 2006) : 235 - 241

ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

C. Kole··, B. Mohanty, A. Pa12, Bithika Singh3, P.R. Kole4, Neeiam Shukla4
1Laboratory of Molecular Biology and Biotechnology, QUAT, Bhubaneswar - 751 003, India

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Cite article:- Kole·· C., Mohanty B., Pa12 A., Singh3 Bithika, Kole4 P.R., Shukla4 Neeiam (2024). ELECTROPHORETIC STUDIES ON SEED PROTEIN PROFILE OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Legume Research. 29(4): 235 - 241. doi: .

ABSTRACT

SDS-polyacrylamide gel electrophoresis of seed globulins of 24 blackgram genotypes displayed the presence of 12 distinctly scoreable polypeptide bands dispersed over four zones, A to D, with Rm values ranging fram 0.05 to 0.821. Five of these polypeptides (Rm: 0.05, 0.128, 0.171, 0.435 and 0.464) varied for their expression. A globulin profile was depicted for the first time based on the electrophoretic banding pattern for this crop. Classificatory analysis led to the identification of the two genptypes, AKU 7 and LBG 402 for their unique protein profiles. The dendrogram constructed revealed, in general, biochemical proximity of the genotypes developed at a particular location and sharing related pedigree. The results generated potentially useful information for future works aiming at elaborated biochemical delineation and their use in improving seed quality in blackgram.

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