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Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 2 (april 2013) : 180-187

REGENERATION IN CULTIVATED (CYAMOPSISTETRAGONOLOBA L.) AND WILD SPECIES (C. SERRATA) OF GUAR

S
S. Mathiyazhagan
S
S.K. Pahuja
A
Anju Ahlawat*
1Forage Section, Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar -125 004, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Mathiyazhagan S., Pahuja S.K., Ahlawat* Anju (2025). REGENERATION IN CULTIVATED (CYAMOPSISTETRAGONOLOBA L.) AND WILD SPECIES (C. SERRATA) OF GUAR. Legume Research. 36(2): 180-187. doi: .

ABSTRACT

Different guar cultivars and the wild species of explants such as the hypocotyls, cotyledon, and mature embryo were used for callus induction and these explants along with cotyledonary node explants were used for direct shoot morphogenesis. It was established that Murashige and Skoog culture medium containing 2,4- D ( 2.5 mg/l) and 6-benzylaminopurine (0.5 mg/l) gave the highest frequency of callus  induction using mature embryo explants in guar cultivars as well as wild  species. Over 2-3 shoots per explants were observed via somatic embryogenesis on MS medium supplemented with NAA 2.0 mg/l+ BAP 0.5mg/l+ Charcoal 3g/l)) in cultivars HG 563 and FS 277 using mature embryo as explants. Direct shoot regeneration was observed using cotyledonary node explants in guar cultivars and its wild species on MS medium containing Kinetin (1.0 mg/l )+ BAP 0.5 (mg/l) and maximum 2-3of shoots per explants were observed on medium supplemented with Kinetin (1.0 mg/l )+ BAP 0.5 (mg/l) and Zeatin (1.0mg/l ). No shoot regeneration was observed in cotyledon, mature embryo, hypocotyl explants of the guar cultivars and the wild species. In vitro rooting was observed on somatic embryo derived shoots on a half-strength concentration of Murashige and Skoog’s culture medium fortified with Indole-3-butyric acid (0.5 mg/l) + charcoal (3g/l).

REFERENCES

  1. Anonymous. (2010). Agricultural Statistics at a glance (http://www.commodityonline.com) Ministry of Agriculture, Govt. of India.
  2. Babber. S.B., Jain, R and Walia, N. (2005) Guar gum as a gelling agent for plant tissue culture media, In Vitro Cell.Dev.Biol.Plant, 41:258-261.
  3. Bansal, Y.K., Chibbar, T., Banslal, S. and Singh M. (1994) Plantlet regeneration from hypocotyls and cotyledon explant of clusterbean (CyamposistetragonolobaL.).J. Phytological Res., 7: 57-60.
  4. Hoque, M. I. and Sarker, R. H. (2007) In vitro plant regeneration in mungbean (Vignaradiata (L.)Wilczek), Plant Tissue Culture & Biotechnology, 17(2): 209-216.
  5. Kaviraj, C.P., Kiran, G., Venugopal, R.B., Kishor, P.B.K. and Srinath, R (2006) Somatic embryogenesis and plant regeneration from cotyledonary explants of green gram [Vignaradiata (L.)Wilezek.] - A recalcitrant grain legume, In Vitro Cellular & Developmental Biology Plant, 42(2):134-138.
  6. Mundhara, R. and Rashid, A. (2006) Recalcitrant grain legume Vignaradiata, mungbean made to regenerate on change of hormonal and cultural conditions. Plant Cell Tiss.Org. Cult.,85: 265–270.
  7. Murashinge, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiol. Plant 10: 949-966.
  8. Pahuja, S. K., Singh, J. V., Jhorar, B. S., Khatri, R. S., Gupta, P. P., Arora, R. N., Singh, R. A., Yadav, R., Phogat, D. S., Jindal, Y. and Pundir, S. R. (2009). Crop improvement scenario in field crops: Success and challenges, 87. Edited by: Sethi, S. K., Chhabra, A. K., Raj, K., Yadav, B. S. and Singh, D.
  9. Prem, D., Subhadra, S., Gupta, P.P., Singh, J.V. and Yadav, G. (2003) High frequency multiple shoot regereration from cotyledonary nodes of guar.In vitro cellular and developmental Biol. Plant,39: 384-387.
  10. Prem, D., Subhadra, Singh., Gupta, P.P., Singh, J.V. and Kadyan, S.P.S. (2005) Callus induction and denovo regeneration from callus in guar (Cyamposistetragonoloba). Plant Cell Tissue and Organ Culture,80: 209-214.
  11. Reichert, N.A., Young, M.M. and Woods, A.L. (2003) Adventitiousorganogenic regeneration from soyabean genotypes representing nine maturity groups. Plant Cell Tissue Organ Cult.,75: 273-277.
  12. Surekha, C. H. and Arundhati, A. (2007) Induction of multiple shoots via organogenesis and plant regeneration from cotyledons of pigeonpea (Cajanuscajan L), Journal of Phytological Research, 20(1):23-27.
  13. Varalaxmi, Y., Vijayalakshmi, A., Yadav, S. K., Venkateswarlu, B.and Maheswari, M. (2007) Efficient plant regeneration from cotyledons of black gram [Vignamungo (L.) Hepper], Indian Journal of Biotechnology,6(3):414-417.
  14. Virender Singh, (2008). Plant regeneration and embryo rescue for interspecific hybrization in guar (Cyamopsistetragonoloba (L) Taub) Ph.D. thesis, CCSHAU, Hisar.
  15. Zambre, M.A., De Clercq. , E., Vranova; Montagu, M.V.G., Angenon. W. and Dillen (1998) Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A-Fray (tepary bean).Plant Cell Reports, 17: 626-630.
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    volume 36 issue 2 (april 2013) : 180-187

    REGENERATION IN CULTIVATED (CYAMOPSISTETRAGONOLOBA L.) AND WILD SPECIES (C. SERRATA) OF GUAR

    S
    S. Mathiyazhagan
    S
    S.K. Pahuja
    A
    Anju Ahlawat*
    1Forage Section, Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar -125 004, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Mathiyazhagan S., Pahuja S.K., Ahlawat* Anju (2025). REGENERATION IN CULTIVATED (CYAMOPSISTETRAGONOLOBA L.) AND WILD SPECIES (C. SERRATA) OF GUAR. Legume Research. 36(2): 180-187. doi: .

    ABSTRACT

    Different guar cultivars and the wild species of explants such as the hypocotyls, cotyledon, and mature embryo were used for callus induction and these explants along with cotyledonary node explants were used for direct shoot morphogenesis. It was established that Murashige and Skoog culture medium containing 2,4- D ( 2.5 mg/l) and 6-benzylaminopurine (0.5 mg/l) gave the highest frequency of callus  induction using mature embryo explants in guar cultivars as well as wild  species. Over 2-3 shoots per explants were observed via somatic embryogenesis on MS medium supplemented with NAA 2.0 mg/l+ BAP 0.5mg/l+ Charcoal 3g/l)) in cultivars HG 563 and FS 277 using mature embryo as explants. Direct shoot regeneration was observed using cotyledonary node explants in guar cultivars and its wild species on MS medium containing Kinetin (1.0 mg/l )+ BAP 0.5 (mg/l) and maximum 2-3of shoots per explants were observed on medium supplemented with Kinetin (1.0 mg/l )+ BAP 0.5 (mg/l) and Zeatin (1.0mg/l ). No shoot regeneration was observed in cotyledon, mature embryo, hypocotyl explants of the guar cultivars and the wild species. In vitro rooting was observed on somatic embryo derived shoots on a half-strength concentration of Murashige and Skoog’s culture medium fortified with Indole-3-butyric acid (0.5 mg/l) + charcoal (3g/l).

    REFERENCES

    1. Anonymous. (2010). Agricultural Statistics at a glance (http://www.commodityonline.com) Ministry of Agriculture, Govt. of India.
    2. Babber. S.B., Jain, R and Walia, N. (2005) Guar gum as a gelling agent for plant tissue culture media, In Vitro Cell.Dev.Biol.Plant, 41:258-261.
    3. Bansal, Y.K., Chibbar, T., Banslal, S. and Singh M. (1994) Plantlet regeneration from hypocotyls and cotyledon explant of clusterbean (CyamposistetragonolobaL.).J. Phytological Res., 7: 57-60.
    4. Hoque, M. I. and Sarker, R. H. (2007) In vitro plant regeneration in mungbean (Vignaradiata (L.)Wilczek), Plant Tissue Culture & Biotechnology, 17(2): 209-216.
    5. Kaviraj, C.P., Kiran, G., Venugopal, R.B., Kishor, P.B.K. and Srinath, R (2006) Somatic embryogenesis and plant regeneration from cotyledonary explants of green gram [Vignaradiata (L.)Wilezek.] - A recalcitrant grain legume, In Vitro Cellular & Developmental Biology Plant, 42(2):134-138.
    6. Mundhara, R. and Rashid, A. (2006) Recalcitrant grain legume Vignaradiata, mungbean made to regenerate on change of hormonal and cultural conditions. Plant Cell Tiss.Org. Cult.,85: 265–270.
    7. Murashinge, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiol. Plant 10: 949-966.
    8. Pahuja, S. K., Singh, J. V., Jhorar, B. S., Khatri, R. S., Gupta, P. P., Arora, R. N., Singh, R. A., Yadav, R., Phogat, D. S., Jindal, Y. and Pundir, S. R. (2009). Crop improvement scenario in field crops: Success and challenges, 87. Edited by: Sethi, S. K., Chhabra, A. K., Raj, K., Yadav, B. S. and Singh, D.
    9. Prem, D., Subhadra, S., Gupta, P.P., Singh, J.V. and Yadav, G. (2003) High frequency multiple shoot regereration from cotyledonary nodes of guar.In vitro cellular and developmental Biol. Plant,39: 384-387.
    10. Prem, D., Subhadra, Singh., Gupta, P.P., Singh, J.V. and Kadyan, S.P.S. (2005) Callus induction and denovo regeneration from callus in guar (Cyamposistetragonoloba). Plant Cell Tissue and Organ Culture,80: 209-214.
    11. Reichert, N.A., Young, M.M. and Woods, A.L. (2003) Adventitiousorganogenic regeneration from soyabean genotypes representing nine maturity groups. Plant Cell Tissue Organ Cult.,75: 273-277.
    12. Surekha, C. H. and Arundhati, A. (2007) Induction of multiple shoots via organogenesis and plant regeneration from cotyledons of pigeonpea (Cajanuscajan L), Journal of Phytological Research, 20(1):23-27.
    13. Varalaxmi, Y., Vijayalakshmi, A., Yadav, S. K., Venkateswarlu, B.and Maheswari, M. (2007) Efficient plant regeneration from cotyledons of black gram [Vignamungo (L.) Hepper], Indian Journal of Biotechnology,6(3):414-417.
    14. Virender Singh, (2008). Plant regeneration and embryo rescue for interspecific hybrization in guar (Cyamopsistetragonoloba (L) Taub) Ph.D. thesis, CCSHAU, Hisar.
    15. Zambre, M.A., De Clercq. , E., Vranova; Montagu, M.V.G., Angenon. W. and Dillen (1998) Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A-Fray (tepary bean).Plant Cell Reports, 17: 626-630.
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