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Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 2 (april 2013) : 138-141

COAT PROTEIN GENE SEQUENCE BASED DIAGNOSIS OF GROUNDNUT BUD NECROSIS VIRUS INFECTION IN RAJMASH

M
Mohd Akram*
N
Naimuddin
1Division of Crop Protection Indian Institute of Pulses Research, Kanpur-208 024, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Akram* Mohd, Naimuddin (2025). COAT PROTEIN GENE SEQUENCE BASED DIAGNOSIS OF GROUNDNUT BUD NECROSIS VIRUS INFECTION IN RAJMASH. Legume Research. 36(2): 138-141. doi: .

ABSTRACT

A disease of rajmash characterized by chlorotic spots with irregular margins, veinal necrosis and slight distortion of leaf lamina was observed during post-rainy season (rabi) of 2009-10, 2010-11 and 2011-12. Plants infected at early stage remained stunted and finally collapsed. Disease incidence was between 4-5% in different fields. The causal virus was successfully sap transmitted from field infected plants to healthy plants of rajmash. The identity of the virus as Groundnut bud necrosis virus (GBNV) was confirmed by reverse transcription-polymerase chain reaction using primer pair (HRP26/HRP28) specific to the coat protein (CP) gene of the GBNV. Complete CP gene of rajmash isolate of GBNV was 831 nucleotides long.  Sequence analysis of the CP gene revealed that the rajmash isolate under study (JN662492) shared maximum identity (98%) at both nucleotide as well as amino acids levels with GBNV-[Mung_ND] (AY871098) and GBNV-[Hya_Mah] (AY882004). The phylogenetic relationship analysis revealed that the present isolate of GBNV clustered with other isolates of GBNV. Thus, the virus causing disease in rajmash at Kanpur was identified as an isolate of GBNV and has been designated as GBNV-[Frb-KNP]. Full sequence of the CP gene of GBNV isolate infecting Phaseolus vulgaris is being reported here for the first time.

REFERENCES

  1. Akram M, Jain RK, Vikas Chaudhary, Ahlawat YS and Khurana SMP. (2004). Comparison of Groundnut bud necrosis virus isolates based on movement protein (NSm) gene sequences. Ann. App. Biol. 145: 285-289.
  2. Akram M and Naimuddin. (2009). Movement protein (NSm) gene sequence based characterization of Groundnut bud necrosis virus infecting cowpea. J. Food Legumes 22: 82-85.
  3. Akram M and Naimuddin. (2010). First report of Groundnut bud necrosis virus infecting pea (Pisum sativum) in India. New Dis. Rep. 21:10. [http://dx.doi.org/10.5197/j.2044-0588.2010.021.010]
  4. Ali, M. and Lal, S. (1991). Rabi Rjmash: Potentialities, limitations and technology of production. Technical No. 3, pp 17, Directorate of Pulses Research, Kanpur 208 024.
  5. Bhat AI, Jain RK, Varma A, Naresh Chandra and Lal SK. (2001). Tospovirus(es) infecting grain legumes in Delhi, their identification by serology and nucleic acid hybridization. Indian Phytopath. 54:112-116.
  6. Jain RK, Bag S, Umamaheshwaran K and Mandal B. (2007). Natural infection by Tospoviruses of Cucurbitaceous and Fabaceous Vegetable Crops in India. J.Phytopath.155:22-25.
  7. Kunkalikar SR, Poojari S, Arun BM, Rajagopalan PA, Chen Tsung-Chi, Yeh Shyi-Dong, Naidu RA, Zehr UB, and Ravi KS. (2011). Importance and Genetic Diversity of Vegetable-Infecting Tospoviruses in India. Phytopathology 101:367-376
  8. Moyer JW. (1999). Tospoviruses (Bunyaviridae). In, Encyclopedia of Virology (Webster, Granoff (Eds.). Academic Press, New York. Pp. 1803-1807.
  9. Mumford RA, Barker I and Wood KR. (1996). The biology of the tospoviruses. Ann. App. Biol. 128: 159-183.
  10. Naimuddin, Mohd Akram and Duraimurugan, P. (2009). Detection of a tospovirus associated with necrotic leaf curl disease of mungbean at Kanpur. J. Food Legumes 22: 86-90.
  11. Reddy DVR, Ratna AS, Sudarshana MR, Poul F and Kirankumar I. (1992). Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Ann. App. Biol. 120:279- 286.
  12. Sivaprasad Y, Bhaskara Reddy BV, Naresh Kumar CVM, Raja Reddy K and Sai Gopal DVR. (2011). Jute (Corchorus capsularis): a new host of Peanut bud necrosis virus. New Dis. Rep. 23:33. [http://dx.doi.org/10.5197/j.2044- 0588.2011.023.033].
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    volume 36 issue 2 (april 2013) : 138-141

    COAT PROTEIN GENE SEQUENCE BASED DIAGNOSIS OF GROUNDNUT BUD NECROSIS VIRUS INFECTION IN RAJMASH

    M
    Mohd Akram*
    N
    Naimuddin
    1Division of Crop Protection Indian Institute of Pulses Research, Kanpur-208 024, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Akram* Mohd, Naimuddin (2025). COAT PROTEIN GENE SEQUENCE BASED DIAGNOSIS OF GROUNDNUT BUD NECROSIS VIRUS INFECTION IN RAJMASH. Legume Research. 36(2): 138-141. doi: .

    ABSTRACT

    A disease of rajmash characterized by chlorotic spots with irregular margins, veinal necrosis and slight distortion of leaf lamina was observed during post-rainy season (rabi) of 2009-10, 2010-11 and 2011-12. Plants infected at early stage remained stunted and finally collapsed. Disease incidence was between 4-5% in different fields. The causal virus was successfully sap transmitted from field infected plants to healthy plants of rajmash. The identity of the virus as Groundnut bud necrosis virus (GBNV) was confirmed by reverse transcription-polymerase chain reaction using primer pair (HRP26/HRP28) specific to the coat protein (CP) gene of the GBNV. Complete CP gene of rajmash isolate of GBNV was 831 nucleotides long.  Sequence analysis of the CP gene revealed that the rajmash isolate under study (JN662492) shared maximum identity (98%) at both nucleotide as well as amino acids levels with GBNV-[Mung_ND] (AY871098) and GBNV-[Hya_Mah] (AY882004). The phylogenetic relationship analysis revealed that the present isolate of GBNV clustered with other isolates of GBNV. Thus, the virus causing disease in rajmash at Kanpur was identified as an isolate of GBNV and has been designated as GBNV-[Frb-KNP]. Full sequence of the CP gene of GBNV isolate infecting Phaseolus vulgaris is being reported here for the first time.

    REFERENCES

    1. Akram M, Jain RK, Vikas Chaudhary, Ahlawat YS and Khurana SMP. (2004). Comparison of Groundnut bud necrosis virus isolates based on movement protein (NSm) gene sequences. Ann. App. Biol. 145: 285-289.
    2. Akram M and Naimuddin. (2009). Movement protein (NSm) gene sequence based characterization of Groundnut bud necrosis virus infecting cowpea. J. Food Legumes 22: 82-85.
    3. Akram M and Naimuddin. (2010). First report of Groundnut bud necrosis virus infecting pea (Pisum sativum) in India. New Dis. Rep. 21:10. [http://dx.doi.org/10.5197/j.2044-0588.2010.021.010]
    4. Ali, M. and Lal, S. (1991). Rabi Rjmash: Potentialities, limitations and technology of production. Technical No. 3, pp 17, Directorate of Pulses Research, Kanpur 208 024.
    5. Bhat AI, Jain RK, Varma A, Naresh Chandra and Lal SK. (2001). Tospovirus(es) infecting grain legumes in Delhi, their identification by serology and nucleic acid hybridization. Indian Phytopath. 54:112-116.
    6. Jain RK, Bag S, Umamaheshwaran K and Mandal B. (2007). Natural infection by Tospoviruses of Cucurbitaceous and Fabaceous Vegetable Crops in India. J.Phytopath.155:22-25.
    7. Kunkalikar SR, Poojari S, Arun BM, Rajagopalan PA, Chen Tsung-Chi, Yeh Shyi-Dong, Naidu RA, Zehr UB, and Ravi KS. (2011). Importance and Genetic Diversity of Vegetable-Infecting Tospoviruses in India. Phytopathology 101:367-376
    8. Moyer JW. (1999). Tospoviruses (Bunyaviridae). In, Encyclopedia of Virology (Webster, Granoff (Eds.). Academic Press, New York. Pp. 1803-1807.
    9. Mumford RA, Barker I and Wood KR. (1996). The biology of the tospoviruses. Ann. App. Biol. 128: 159-183.
    10. Naimuddin, Mohd Akram and Duraimurugan, P. (2009). Detection of a tospovirus associated with necrotic leaf curl disease of mungbean at Kanpur. J. Food Legumes 22: 86-90.
    11. Reddy DVR, Ratna AS, Sudarshana MR, Poul F and Kirankumar I. (1992). Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Ann. App. Biol. 120:279- 286.
    12. Sivaprasad Y, Bhaskara Reddy BV, Naresh Kumar CVM, Raja Reddy K and Sai Gopal DVR. (2011). Jute (Corchorus capsularis): a new host of Peanut bud necrosis virus. New Dis. Rep. 23:33. [http://dx.doi.org/10.5197/j.2044- 0588.2011.023.033].
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