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Current IssueEditorial BoardOnline First ArticlesArchive

volume 32 issue 4 (december 2009) : 265-269

ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS

S
Sonali Guleria
S
Saroj Dua
N
Nirmala Chongtham
1Department of Botany, Panjab University, Chandigarh-160 014, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Guleria Sonali, Dua Saroj, Chongtham Nirmala (2025). ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS. Legume Research. 32(4): 265-269. doi: .

ABSTRACT

Seeds of 10 varieties of pea procured from Punjab Agricultural University, Ludhiana and
Haryana Agricultural University, Hissar, India and grown in research farm at Panjab University,
Chandigarh for three successive generations were analyzed for total protein and banding patterns
of protein fractions. Significant varietal variations in the mature dry seeds of pea genotypes for
total protein (14.95% to 22.44%), albumins (3.30% to 6.35%), globulins (7.97% to 10.53 %),
glutelins (1.15% to 3.05 %) and prolamins (0.46% to 1.50 %) were observed for the means of
three years. Seed storage protein banding profile was also studied by performing SDSPolyacrylamide
gel electrophoresis. A total of 20-21 bands were observed in all the ten genotypes.
Except for some minor differences in the intensity of the bands, major differences were not
recorded in the protein profiles.

REFERENCES

  1. Boulter D. et al. (1987). Pl. Physiol. Biochem. 25:283-289.
  2. Boulter D. and Croy R.R.D. (1997). Adv. Bot. Res. 27:1-84.
  3. Casey R. et al. (1982). Pl. Foods Hum. Nutr. 31:33-346.
  4. Collada C. et al. (1991). J. Exp. Bot. 42:1305-1310.
  5. Cooke R.J., (1986). Electrophoresis 7:203-217.
  6. Gottschalk W. et al. (1975). Egypt J. Genet. Cytol. 4:453-468.
  7. Hames B.O. and Rickwood D. (1990). Gel Electrophoresis of Proteins; a Practical Approach. 2nd ed. Oxford University Press, U.S.A.
  8. Higgins T.J.V. (1984). Ann. Rev. Pl. Physiol. 35:191-221.
  9. Ignacimuthu, S. and Arockiadass A. (1993). Madras Agric. J. 80:252-254.
  10. Iqbal A. et al. (2006). Food Chem. 97:331-35.
  11. Kumar P. et al. (2004). Haryana J. Hort. Sci. 33:243-45.
  12. Ladizinsky G. and Hymowitz T. (1979). Theor. Appl. Genet. 51:145-151.
  13. Laemmli U.K. (1970). Nature. 227:680-685.
  14. Lowry O.H. et al. (1951). J. Biol. Chem. 193:265-275.
  15. Mariotti F. et al. (2001). J. Nutr. 131:1706-1713.
  16. Matta N.K. and Gatehouse J.A. (1982). Heredity. 48:383-392.
  17. McPhee K. (2003). Food Agric. & Environ. 1:64-69.
  18. Miller M.K. et al. (1972). Crop. Sci. 12:535- 537.
  19. Naik M.S. (1968). Indian J. Genet. 28:142-146.
  20. Sathe S.K. (2002). Cr. Rev. in Biotech. 22:175-223.
  21. Shinde K.G. (2000). Orissa J. Hort. 28:21-24.
  22. Sureja A.K. and Sharma R.R. (2000). Indian J. Hort. 57:243-47.
  23. Turner S.R. et al. (1990). Pl. Mol. Biol. 14:793-803.
  24. Valizadeh M. (2001). J. Agric. Sci. Technol. 3:287-92.
  25. Vandana A.T. and Dubey D.K. (1994). Lens. Newsl. 21:16-18.
  26. Welch R.M. (2005). Food. Nutr. Bull. 26:419-21.
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    volume 32 issue 4 (december 2009) : 265-269

    ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS

    S
    Sonali Guleria
    S
    Saroj Dua
    N
    Nirmala Chongtham
    1Department of Botany, Panjab University, Chandigarh-160 014, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Guleria Sonali, Dua Saroj, Chongtham Nirmala (2025). ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS. Legume Research. 32(4): 265-269. doi: .

    ABSTRACT

    Seeds of 10 varieties of pea procured from Punjab Agricultural University, Ludhiana and
    Haryana Agricultural University, Hissar, India and grown in research farm at Panjab University,
    Chandigarh for three successive generations were analyzed for total protein and banding patterns
    of protein fractions. Significant varietal variations in the mature dry seeds of pea genotypes for
    total protein (14.95% to 22.44%), albumins (3.30% to 6.35%), globulins (7.97% to 10.53 %),
    glutelins (1.15% to 3.05 %) and prolamins (0.46% to 1.50 %) were observed for the means of
    three years. Seed storage protein banding profile was also studied by performing SDSPolyacrylamide
    gel electrophoresis. A total of 20-21 bands were observed in all the ten genotypes.
    Except for some minor differences in the intensity of the bands, major differences were not
    recorded in the protein profiles.

    REFERENCES

    1. Boulter D. et al. (1987). Pl. Physiol. Biochem. 25:283-289.
    2. Boulter D. and Croy R.R.D. (1997). Adv. Bot. Res. 27:1-84.
    3. Casey R. et al. (1982). Pl. Foods Hum. Nutr. 31:33-346.
    4. Collada C. et al. (1991). J. Exp. Bot. 42:1305-1310.
    5. Cooke R.J., (1986). Electrophoresis 7:203-217.
    6. Gottschalk W. et al. (1975). Egypt J. Genet. Cytol. 4:453-468.
    7. Hames B.O. and Rickwood D. (1990). Gel Electrophoresis of Proteins; a Practical Approach. 2nd ed. Oxford University Press, U.S.A.
    8. Higgins T.J.V. (1984). Ann. Rev. Pl. Physiol. 35:191-221.
    9. Ignacimuthu, S. and Arockiadass A. (1993). Madras Agric. J. 80:252-254.
    10. Iqbal A. et al. (2006). Food Chem. 97:331-35.
    11. Kumar P. et al. (2004). Haryana J. Hort. Sci. 33:243-45.
    12. Ladizinsky G. and Hymowitz T. (1979). Theor. Appl. Genet. 51:145-151.
    13. Laemmli U.K. (1970). Nature. 227:680-685.
    14. Lowry O.H. et al. (1951). J. Biol. Chem. 193:265-275.
    15. Mariotti F. et al. (2001). J. Nutr. 131:1706-1713.
    16. Matta N.K. and Gatehouse J.A. (1982). Heredity. 48:383-392.
    17. McPhee K. (2003). Food Agric. & Environ. 1:64-69.
    18. Miller M.K. et al. (1972). Crop. Sci. 12:535- 537.
    19. Naik M.S. (1968). Indian J. Genet. 28:142-146.
    20. Sathe S.K. (2002). Cr. Rev. in Biotech. 22:175-223.
    21. Shinde K.G. (2000). Orissa J. Hort. 28:21-24.
    22. Sureja A.K. and Sharma R.R. (2000). Indian J. Hort. 57:243-47.
    23. Turner S.R. et al. (1990). Pl. Mol. Biol. 14:793-803.
    24. Valizadeh M. (2001). J. Agric. Sci. Technol. 3:287-92.
    25. Vandana A.T. and Dubey D.K. (1994). Lens. Newsl. 21:16-18.
    26. Welch R.M. (2005). Food. Nutr. Bull. 26:419-21.
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