Legume Research

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Legume Research, volume 32 issue 4 (december 2009) : 265-269

ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS

Sonali Guleria, Saroj Dua, Nirmala Chongtham
1Department of Botany, Panjab University, Chandigarh-160 014, India.

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Cite article:- Guleria Sonali, Dua Saroj, Chongtham Nirmala (2024). ANALYSIS OF VARIABILITY IN DIFFERENT GENOTYPES OF PEA (PISUM SATIVUM L.) ON THE BASIS OF PROTEIN MARKERS. Legume Research. 32(4): 265-269. doi: .

ABSTRACT

Seeds of 10 varieties of pea procured from Punjab Agricultural University, Ludhiana and
Haryana Agricultural University, Hissar, India and grown in research farm at Panjab University,
Chandigarh for three successive generations were analyzed for total protein and banding patterns
of protein fractions. Significant varietal variations in the mature dry seeds of pea genotypes for
total protein (14.95% to 22.44%), albumins (3.30% to 6.35%), globulins (7.97% to 10.53 %),
glutelins (1.15% to 3.05 %) and prolamins (0.46% to 1.50 %) were observed for the means of
three years. Seed storage protein banding profile was also studied by performing SDSPolyacrylamide
gel electrophoresis. A total of 20-21 bands were observed in all the ten genotypes.
Except for some minor differences in the intensity of the bands, major differences were not
recorded in the protein profiles.

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