EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET

¹Key Laboratory of New Animal Drug Project,Gansu province/ Key Laboratory of Veterinary Pharmaceutics Discovery ,Agricultural Ministry/ Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Lanzhou 730050

ABSTRACT

Locoweed is a poisonous plantÿwhich is wildly distributed in most area of the worldÿand cause livestock poisoning or death with serious economic loss. The Astragalusstrictus belongs to a species of locoweed .It is mainly distributed in Tibet of China and is a serious hazard to the local livestock industry. The objective of this study is extracting and purifying condition of Swainsonine fromAstragalusstrictuswith the microwave-assisted extraction method. An optimum extracting technology of SW fromAstragalusstrictuswas investigated through the orthogonal experiment under the microwave assistance conditions, then the content of swainsonine was analyzed with gas chromatographyÿGC method. The optimized extraction conditions were as follow: at room temperature, the time of using the petroleum ether ultrasonic to make the Swainsonine degreased with ultrasonic was 1h, and then use the method of extraction with microwave-assisted, solid-liquid ratio (M : V) 1:10, microwave frequencies 280W , extraction time 20 s. Under the above conditions, the extraction percentage of the swainsonine was 0.009264%. Extracting swainsonine with microwave-assisted extraction method has characteristics of rapid, efficient, convenient and low energy consumption. It is beneficial for producing swainsonine fromAstragalusstrictus.

KEYWORDS

REFERENCES

Colegate,S.M., Dorling,P.R. and Huxtable, C.R. (1979). A spectroscopic investigation of swainsonine: an a mannosidase inhibitor isolated from Swainsinacanecens. Aust J Chem. 32:2257-2264.
Dennis, J.W., Koch, K.,Yousefi, S. and Vanderelst, I. (1990).Growth inhibition of human melanoma tumor xenografts in athymic nude mice by swainsonine. Cancer Res. 50(6):1867-1872.
Guo,X.W., Zhang,F.C. and Lin,S.Y. (1993). The application of ultrasound technology in the extraction of herbal ingredients. Chinese Herbal Medicines.24(10): 548-550.
James,L.F. and Hartley,W.J. (1977). Effect s of milk from animals feed locoweed on kittens, calves and lambs. Am J Vet Res. 38:1263-1265.
Li, J.K. (2003) .Research and prospect of locoweed of China. Agric. Sci. China.36(9):1091-1099.
Liu,Z.Y., Zhao,B.Y., Sun,L.S,,Wan,X.P. and Huo, X.H. (2008). Research advances in swainsonine resources and its detection methods. Chinese Vet. Sci. 38(02):175-180.
Lu,H.(WangÿJ.J., Sun,L.S.)Zhao,B.Y., Wang and Z.X., Ma,R. (2009). Progress on Antineoplastic Activity of Swainsonine. Prog. Vet. Medicine. 30(9) :87-90.
Song,Y.M., Wang,J.H., Wang,A.H., Wang,Y. and Yu,Y.T. (2010).The determination of swainsonine in rabbit serum by gas chromatography. Chinese Journal of Veterinary Science. 30(3): 402-405.
Tong, D.W. (2000).Studies on synthesis of swainsonine of swainsonine - BSA and its immunogenicity.Northwest A & F University, PhD thesis of Materials physics and chemistry professional.
Wang,K., MO,C.H., Zhao,B.Y. and Cao,G.R.. (1999).”Jifang No. E” prevention sheep Poisoning of Gansu oxytropis.Anim. Husb. Vet. Medicine. 3:31-32.
Wang,S., Chen,G.Y., Hu,J.J. and Ma,C.H. (2011). Determination of Swainsonine Content in Oxytrpisglabra DC in South Xinjiang by Internal Standard Gas Chromatography. Xinjiang Agric. Sci.48(4):728-733.
Wu, D., Liang, B., Feng, Z.G. and Shi,Y.P. (2003). Research progress of swainsonine. Chinese Traditional Vet. Medicine.2:42-45.
Yang, M. Q.,Cao (G.R.,Li), S.J. (1998). Extraction of swainsonine of the beans Rhizoctonia. J. Northwestern Agric. Univ. 26(4): 87-90.
Zhao B.Y., Fan, Y.Y. Fan, Z.F. Dong, Q. and Cao, G.R. (2006). Hazards of locoweed in the west grassland of China and the control of animals loco-toxicosis. Grass-Feeding Livestock.130:12–15.
Zhao, B.Y.. (2007).The Northwest Agricultural developed grassland poisonous locoweed antidote.1:66.
Zhao,J.,Tong, D.W., Shui, Y.Y., Zhao, B.Y., Liu, W.M. and Li,L. (2006).Compariso-n of Swainsonine-Extracting Technologies from Oxytropiskansuensis Bunge.ActaBotanicaBoreali-OccidentaliaSinica.26(7):1459-1463.
Zeng,L. and Xia,Z.N. (2002).The improvement and influence of ultrasonic and microwave irradiation on the extraction of traditional Chinese medicine. Chemical Res. Application. 14(3): 245-249.
Zhao, X.H., He,X. and Wang, J.H. (2011).Determination of Swainsonine of Astragalusvariabilis Bunge by Gas Chromatography with Solvent Extraction. Acta Agriculturae Boreali-Occidentalis Sinica. 20(6):71-74

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

EXTRACTION AND GC STUDY ON SWAINSONINE FROM LOCOWEED ASTRAGALUS STRICTUS IN TIBET

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board