Legume Research

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Legume Research, volume 36 issue 1 (february 2013) : 21-32

EVALUATION OF ANTIOXIDANT ACTIVITY, CYTOTOXIC STUDIES AND GC-MS PROFILING OF Matthiola incana (STOCK FLOWER)

Nasir Rasool1*, Sania Afzal1, Muhammad Riaz1, Umer Rashid2* Komal Rizwan1, Muhammad Zubair1, Shafqat Ali3, Muhammad Shahid4
1Department of Chemistry, Government College University Faisalabad-384 00, Pakistan

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Cite article:- Rasool1* Nasir, Afzal1 Sania, Riaz1 Muhammad, Rizwan1 Komal Rashid2* Umer, Zubair1 Muhammad, Ali3 Shafqat, Shahid4 Muhammad (2025). EVALUATION OF ANTIOXIDANT ACTIVITY, CYTOTOXIC STUDIES AND GC-MS PROFILING OF Matthiola incana (STOCK FLOWER). Legume Research. 36(1): 21-32. doi: .

ABSTRACT

Antioxidant activity of Matthiola incana flowers extracted with different solvents like petroleum ether, chloroform, ethyl acetate, absolute methanol, methanol water (9.5:0.5) methanol water (9:1) extracts of stock flower (Matthiola incana) were analyzed. The total phenolics content (TPC) were found in the range of 0.327-1.872 mg GAE/g and total flavonoids content (TFC) were found in the range 2.06-6.067 CE mg/g. Other parameters studied were reducing power, DPPH radical scavenging and inhibition of linoleic acid per oxidation. The antioxidant activity of the  Matthiola incana was also evaluated by measuring the conjugated dienes (CD), conjugated triene (CT), p-anisidine value (PAV), free fatty acid (FFA) and peroxide values (PV) by using canola oil as an oxidative substrate. The n-hexhane extract was also analyzed by GC-MS. The methanolic extracts of Matthiola incana flowers showed maximum antioxidant activity. The cytotoxicity studies was also studied by haemolytic activity of the plant extracts against the human erythrocytes (RBC’s) was found to be in the range of 0.91-4.47 %.

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