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Current IssueEditorial BoardOnline First Articles
Legume Research
Print ISSN: 0250-5371
Online ISSN: 0976-0571
NASS Rating
6.80
SJR
0.32
CiteScore
0.906

Chief Editor:
J. S. Sandhu
Vice Chancellor, SKN Agriculture, University, Jobner, VC, NDUAT, Faizabad, Deputy Director General (Crop Science), Indian Council of Agricultural Research (ICAR), New Delhi
Frequency:Monthly
Indexing:
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Go...

Legume Research, volume 36 issue 1 (february 2013) : 15-20

STABLE GUS GENE EXPRESSION IN THREE CHICKPEA VARIETIES VIZ., PRATAP-1, DAHOD YELLOW AND GNG-469

Vinod Saharan*, Vikas Meena, H.K. Jain, Ram C. Yadav1
1Department of Molecular Biology and Biotechnology, M.P. University of Agriculture & Technology, Udaipur-313 001, India

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Cite article:- Saharan* Vinod, Meena Vikas, Jain H.K., Yadav1 C. Ram (2025). STABLE GUS GENE EXPRESSION IN THREE CHICKPEA VARIETIES VIZ., PRATAP-1, DAHOD YELLOW AND GNG-469. Legume Research. 36(1): 15-20. doi: .

ABSTRACT

High frequency stable GUS gene expression has been achieved in three chickpea varieties. Standardized regeneration protocol from epicotyl explant has been used for regeneration of transformed GUS expressing shoots of chickpea. Agrobacterium tumefaciens strain EHA 105 containing vector pCAMBIA 2301 and strain LB 4404 harboring pBI121 were used in present study. Infection time (15 min.), co-cultivation duration (72 hrs.), acetosyringone (200 µM) were found to be optimum for transient and stable GUS gene expression via pCAMBIA vector. Maximum percentage of stable GUS gene expression was recorded 33.3 %  in microshoots of Pratap-1, followed by 19.0 % in microshoots of GNG-469. Efforts have been made to perform PCR for regenerated microshoots to confirmed stable GUS and npt-II gene integration in transformed microshoots. Present protocols developed for popular local varieties of chickpea can be used for transfers of genes for varietal improvement programme.

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