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volume 33 issue 1 (march 2010) : 17 - 22

SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE

L
L. Prasanthi
B
B.V. Bhaskara Reddy
K
K. Rekha Rani
T
T. Rajeswari
Y
Y. Sivaprasad
K
K. Raja Reddy.
1Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517502, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Prasanthi L., Reddy Bhaskara B.V., Rani Rekha K., Rajeswari T., Sivaprasad Y., Reddy. Raja K. (2025). SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE. Legume Research. 33(1): 17 - 22. doi: .

ABSTRACT

Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening
of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified
3 lines having 0-2% plant disease incidence and grouped under 0-1 disease score. PCR reactions
using different primers with genomic DNA of these lines resulted in identification of three resistant
sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer.
Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to
identify the new resistance sources in a short time and they could be utilized in breeding programme
or for direct release.

REFERENCES

  1. Melotto, M..et al.(1996). Genome., 39 : 1212-1219.
  2. Munjal,R.L. et al.(1963). Indian Phytopath. 19:260-261
  3. Murray, M.G. and Thompson,W.F. (1986). Nucleic Acids Res. 8: 4321-4325
  4. Naqvi, N.I. and Chattoo, B.B.( 1996). Genome. 39 : 26-30
  5. Reddy,K.S. et al.(1994). Theoritical App Genet. 88 : 945-948
  6. Sambrook, J.et al.(1989). Molecular Cloning: Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press,
  7. New York.
  8. Sharma, B.(1995). Genetic Research and Education: Current Trends and Next Fifty Years.Indian Society of Genetics
  9. and Plant reeding.New Delhi,India 916-924
  10. Zhag. Y. and Stommet,J.R. (2001). Crop Sci. 41: 1602-1608
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    volume 33 issue 1 (march 2010) : 17 - 22

    SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE

    L
    L. Prasanthi
    B
    B.V. Bhaskara Reddy
    K
    K. Rekha Rani
    T
    T. Rajeswari
    Y
    Y. Sivaprasad
    K
    K. Raja Reddy.
    1Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517502, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Prasanthi L., Reddy Bhaskara B.V., Rani Rekha K., Rajeswari T., Sivaprasad Y., Reddy. Raja K. (2025). SCREENING OF BLACKGRAM GENOTYPES UNDER ARTIFICIALLY INOCULATED CONDITIONS AND BASED ON MOLECULAR MARKERS FOR POWDERY MILDEW RESISTANCE. Legume Research. 33(1): 17 - 22. doi: .

    ABSTRACT

    Powdery mildew is an important disease in blackgram which causes severe yield loses. Screening
    of 15 lines along with LBG 17 resistant check for powdery mildew under artificial inoculation identified
    3 lines having 0-2% plant disease incidence and grouped under 0-1 disease score. PCR reactions
    using different primers with genomic DNA of these lines resulted in identification of three resistant
    sources with specific amplification for resistance to powdery mildew at 900bp with OPT16 primer.
    Considering the powdery mildew reaction and resistance linked RAPD marker, it was possible to
    identify the new resistance sources in a short time and they could be utilized in breeding programme
    or for direct release.

    REFERENCES

    1. Melotto, M..et al.(1996). Genome., 39 : 1212-1219.
    2. Munjal,R.L. et al.(1963). Indian Phytopath. 19:260-261
    3. Murray, M.G. and Thompson,W.F. (1986). Nucleic Acids Res. 8: 4321-4325
    4. Naqvi, N.I. and Chattoo, B.B.( 1996). Genome. 39 : 26-30
    5. Reddy,K.S. et al.(1994). Theoritical App Genet. 88 : 945-948
    6. Sambrook, J.et al.(1989). Molecular Cloning: Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press,
    7. New York.
    8. Sharma, B.(1995). Genetic Research and Education: Current Trends and Next Fifty Years.Indian Society of Genetics
    9. and Plant reeding.New Delhi,India 916-924
    10. Zhag. Y. and Stommet,J.R. (2001). Crop Sci. 41: 1602-1608
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