The effects of Dia-V
TM MB
PRO dietary inclusions
(200 MB
PRO, 400 MB
PRO and 800 MB
PRO) to Ross-308 broiler chicks ration on feed intake, live body weight, feed conversion, carcass traits, serum metabolites and antioxidant capacity are presented in (Fig 2, 3 and 4) and (Table 1).
Growth performances
The effects of dietary inclusions (200 MB
PRO, 400 MB
PRO and 800 MB
PRO) on growth performance are presented in (Fig 2, 3 and 4). The dietary inclusions (200 MB
PRO, 400 MB
PRO and 800 MB
PRO) resulted in significantly higher final body weight (g) compared to the control group (0 MB
PRO) (P<0.03). The Dia-V
TM MB
PRO dietary inclusions resulted in significant growth performance represented by improved body weight gain, feed intake, feed conversion ratio, carcass traits (Fig 2, 3 and 4). In general, dietary inclusions resulted in significant improvement in growth performance parameters concerning live body weight, feed conversion and carcass traits. In addition, 400 MB
PRO dietary inclusions gave the highest values in body weight and dressing percentage compared to other groups. While, 200 mg/kg had better feed conversion ratio compared to all other groups.
Serum metabolites and antioxidant capacity
The effects of different levels of Dia-V
TM MB
PRO in broiler diet on serum metabolites and antioxidant capacity to broiler chickens are presented in (Table 1). The Dia-V
TM MB
PRO dietary inclusions resulted in significantly higher total protein levels compared to the control group (P<0.03). In 200 MB
PRO group, albumin value showed a significantly higher value compared to the control and other Dia-V
TM MB
PRO groups (P<0.04). There was no statistically significant differences in creatinine level among the control and Dia-V
TM MB
PRO groups (P=0.47). Groups 400 MB
PRO and 800 MB
PRO exhibited significantly higher glucose levels compared to the control and 200 MB
PRO groups (P=0.05). The Dia-VTM MBPRO inclusions significantly reduced total bilirubin levels compared to 0 MB
PRO inclusion (P<0.0001). Regrading to AST, ALT and GGT enzymes, there were no significant differences in AST (P=0.54), ALT (P=0.89) and GGT (0.74) levels among the control and Dia-V
TM MB
PRO groups. Values of ALP were significantly (P=0.01) decreased in 400 MB
PRO and 800 MB
PRO groups compared to 200 MB
PRO and control ones. Regarding the antioxidant capacity, the FRAP, FRSA, ABTS and TTL values were not significantly different among groups whereas MDA values were significantly (P=0.001) decreased in Dia-V
TM MB
PRO groups compared to control one.
The results of the current study indicated the highest significant improvement of Dia-V
TM MB
PRO dietary inclusions to Ross-308 broiler chicks on growth performance, serum metabolites and antioxidant capacity at level of 400 mg/kg. The effect of Dia-V
TM MB
PRO could be attributed to several factors including improved gut health, boosted immune system and reduction of pathogens
(Mazziotta et al., 2023; Chandrasekaran et al., 2024).
Growth performances
The effects of
Saccharomyces cerevisiae (
S. cerevisiae) and essential oils dietary inclusions to broiler chicks on growth performances have been explored in several studies
(Lin et al., 2023; Okasha et al., 2023). Our results indicated that dietary 400 mg/kg MB
PRO inclusion gave the highest body weight compared to all groups. Higher feed conversion ratio was recorded for chicks fed diet containing 200 mg/kg compared to other groups. The negative or positive changes of growth performances over
S. cerevisiae supplementation to broiler chicks were dependent on the given doses
(Haldar et al., 2011; Swamy and Upendra 2013).
Okasha et al., (2023) found that 0.25, 0.5 and 0.75%
S. cerevisiae supplementation decreased body weight gain. On the other, significant increase of broilers BW fed diet added containing 0.1, 0.5, 0.75 and 1.0%
S. cerevisiae compared to control one (
Swamy and Upendra 2013;
Okasha et al., 2023).
Additionally, essential oils are characterized by biological useful properties in the diet of broiler chickens reflected in improved growth performance and increase the body’s immune response
(Ezzat et al., 2016; Giannenas et al., 2018; Puvača et al., 2015, 2022).
Collectively, dietary
Saccharomyces cerevisiae and essential oils inclusion might provide comprehensive benefits to poultry of all ages and types, fostering a healthy gut environment that leads to improved digestion and enhanced growth performance
(Lin et al., 2023; Movahedi et al., 2024; Abd El-Ghany 2024). They achieve this by balancing gut bacteria, strengthening the intestinal lining and stimulating the immune system while reducing harmful inflammation. The
S. cerevisiae provides valuable proteins, vitamin B-complex and other beneficial factors that have been reported to increase phosphorus availability and utilization in animals
(Moore et al., 1994; Medina et al., 2002). Furthermore, it has been indicated in several studies that dietary
S. cerevisiae inclusion reduced disease infections and improved feed efficiency
(Line et al., 1997; Santin et al., 2001).
Serum metabolites and antioxidant capacity
The values of serum metabolites and antioxidant capacity (Table 1) were improved in Dia-V
TM MB
PRO groups compared to control group as in other studies (
Paryad and Mahmoudi 2008;
Okasha et al., 2023). Total protein and albumin (g/dl) were significantly increased in Dia-V
TM MB
PRO groups compared to control one. This could be attributed to the significant improvement of feed intake and feed conversion obtained in those groups, which subsequently resulted in increased glucose and creatinine values. Total bilirubin values were decreased (P=0.0001) in Dia-V
TM MB
PRO groups compared to control groups. Both
S. cerevisiae and essential oils have shown potential to improve liver health in poultry. By maintaining healthy liver function, these additives may contribute to efficient bilirubin processing and excretion in addition to unchanged liver enzymes (Table 1).
The antioxidant capacities were gradually decreased in Dia-V
TM MB
PRO groups with increasing doses if compared to the control group except ABTS, µM.
S. cerevisiae contains components, such as beta-glucans, that can stimulate the poultry’s immune system, which enhances antioxidant defenses. Some studies have shown that
S. cerevisiae can enhance the activity of antioxidant enzymes in poultry. Additionally, the essential oils are rich in bioactive compounds, such as terpenes and phenols, which possess potent antioxidant properties and modulate the activity of antioxidant enzymes (
De Sousa et al. 2023;
Sequeda-Castañeda et al., 2025).