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An Evaluation of Zinc Sulphate Toxicity on Protein, Amino Acid and Transaminase Levels in Freshwater Fish, Channa striata (bloch)

DOI: 10.18805/IJAR.B-4259    | Article Id: B-4259 | Page : 1342-1346
Citation :- An Evaluation of Zinc Sulphate Toxicity on Protein, Amino Acid and Transaminase Levels in Freshwater Fish, Channa striata (bloch).Indian Journal of Animal Research.2021.(55):1342-1346
P. Pandari Reddy, G. Sunitha Devi pandureddy29@gmail.com
Address : Department of Zoology, University College of Science, Osmania University, Hyderabad-500 007, Telangana, India.
Submitted Date : 22-07-2020
Accepted Date : 24-12-2020

Abstract

Background: Heavy metals may adversely affect the aquatic flora and fauna. Industrial effluents contributing heavy metals on entering into aquatic environment causes biochemical alterations in the fish. Heavy metals are natural components of the aquatic environment, but heavy metal levels have increased due to anthropogenic sources like industrial effluents, agricultural runoff and wastes of mining activities. All the pollutants affect the physicochemical characteristics of the water bodies, sediment and biological components and thus the quality and quantity of fish stocks. 
Methods: The present study was to assess the protein, amino acid, aspartate transaminase and alanine transaminase levels in muscle and liver tissues of Channa striata, exposure to sublethal (15, 20 and 30 mg/L) concentrations of zinc sulphate for the period of 10, 20, 30 and 40 days. Protein levels were estimated by Lowry et al. (1951). Method free amino acids were estimated by Moore and Stein (1954). Method the enzymes ALT and AST were estimated by the strategy of Rietman and Frankel (1957) as described by Bergmeyer (1965).
Result: The fish exposed to zinc sulphate showed a decrease in the protein levels and increase in the amino acid, AST and ALT levels for 10, 20, 30 and 40 days in muscle and liver tissues. The observed mean data were statistically significant at P< 0.05 of Student's ‘t’ test. The present study indicated alterations in biochemical parameters of Channa striata due to intoxication of zinc sulphate.

Keywords

Amino acid ALT AST Channa striata Liver Muscle Protein Sublethal concentration Zinc sulphate

References

  1. Azmat, R. and Talat, R. (2006). Metal contamination in edible fresh water fishes of Arabian Sea. J. Applied Sci. 6: 1974-1977. 
  2. Bais, U.E and Lokhande, M.V. (2012). Effect of cadium chloride on the biochemical content in different tissues of the fresh water fish, Ophicephalus striatus. International Research Journal of Biological Sciences. 1(7): 55-57.
  3. Bergmeyer, H.U., Bernt, E. and Hess, B. (1965). Lactic dehydrogenase. In Methods of Enzymatic Analysis. Academic Press, pp.736-743. New York,
  4. Farombi, E.O, Adelowo, O.A. and Ajimoko, Y.R. (2007). Biomarker of oxidative effect and heavy metal levels of environmental pollution in Clarias gariepinus from Nigeria Ogun River. Int J Environ Res Public Health. 4: 158-165.
  5. Guven, K., Ozbay Unlu, C., Satar, E. (1999). Acute lethal toxicity and accumulation of copper in [Gammarus pulex (L.) Amphipoda]. Turk. J. Biol. 23: 513-21.
  6. Karra Somaiah and Sunita, K. (2015). Biochemical changes induced by Butachlor, pre-emergent herbicide to sub lethal concentrations in the freshwater fish Labeo rohita (Hamilton). Journal de Afrikana. 2(4): 99Y122.
  7. Kamaraju, S., Ramasamy, K. (2018). Effect of heavy metal, cadmium chloride on protein and amino acid content changes in freshwater exotic fish, Hypopthalmichthys molitrix, Indo Am. JP. Sci. 05(02): 909-915.
  8. Kock, G., Triendl, M. and Hofer, R. (1996). Seasonal patterns of metal accumulation in Arctic char (Salvelinus alpinus) from an oligotrophic Alpine lake associated with temperature. Can. J. Fish. Aquat. Sci. 53:780-786.
  9. Kumari, K. and Sinha R.C. (2011). Effects of carbaryl and lindane on some biochemical parameters of the blood, liver, muscles, kidney and brain of the frog, Rana tigrina. Bioved. 17: 55-65. 
  10. Lowry, O.H. and Rosebrough, N.J. (1951) Protein measurement with folin-phenol reagent. J. Biol. Chem. 193: 265-275.
  11. Magar, R.S. and Afsar Shaikh. (2010). Effect of malathion on respiratory responses of fresh water fish, Channa punctatus. Trends in Fisheries Research. 1.3: 1-4.
  12. Mahalakshmi, D. (1997). Effect of industrial effluents on bioaccumulation and histopathology of a freshwater fish, Oreochromis mossambicus, M.Phil, thesis, Annamalai University, India.
  13. Mc Commis, K.S., Chen, Z., Fu, X., Mc Donald, Colca, J.R, Kletzien R.F. (2015). Loss of mitochondrial pyruvate carrier 2 within the liver results in defects in gluconeogenesis and compensation via pyruvate-alanine cycling. Cell Metab. 22: 682-694.
  14. Moore, S. and Stein, W.H. (1954). Procedure for the chromatographic determination of amino acids on four per cent. Cross- linked sulfonated polystyrene resins. Journal of Biological Chemistry. 25(3). 211: 893-906.
  15. Moorthikumar, K. and Muthulingam, M. (2010). Protein metabolism in liver, kidney and brain of Labeo rohita (Hamilton) under heavy metal, nickel chloride stress. International Journal of Current Research. 7: 014-017. 
  16. Pandey, S., Parvez, S., Sayeed, I., Haque, R., Bin-Hafeez , B. and Raisuddin, S. (2003). Biomarkers of oxidative stress: a comparative study of river Yamuna fish, Wallago attu (Bl. and Schn.). Science of the Total environment. 309(1-3): 105-115.
  17. Prashanth, M.S. and Neelagund, S.E. (2007). Free cyanide induced biochemical alterations in nitrogen metabolism of the Indian major carp. J. Basic. Clin. Physiol. Pharmacol. 18(4): 277-287.
  18. Reitman, S. and Frankel, S. (1957). A colorimetric method for the estimation of serum glutamic oxaloacetate (SGOT) and glutamic pyruvic transaminases (GPOT). Anim. J. Clin. Path. 28: 56-63.
  19. Senthil Elango, P., Muthulingam, M. (2014). Impact of heavy metal chromium on protein and aminoacid contents in brain and muscle of freshwater fish Oreochromis mossambicus (Peters), International Journal of Current Research. 6(01): 4841-4845. 
  20. Tulasi, G. and Jayantha Rao, K. (2013). Effect of chromium on protein metabolism in different tissues of fish, Cyprinus carpio. Res. J. Pharma, Bio and Chemi, Sci. 4(1): 143-148.

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