In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs)

DOI: 10.18805/ijar.B-3992    | Article Id: B-3992 | Page : 530-535
Citation :- In vitro Cytotoxicity Analysis of Hybrid Graphene Oxide (hGO) Nano Structures in Caprine Wharton’s Jelly Derived Mesenchymal Stem Cells (WJ-MSCs).Indian Journal Of Animal Research.2021.(55):530-535
S.A. Dhenge, N.E. Gade, O.P. Mishra, Abinash Kumar, V.N. Khandait drdhenge2009@gmail.com
Address : Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Anjora, Durg-491 001, Chhattisgarh, India.
Submitted Date : 31-01-2020
Accepted Date : 12-08-2020

Abstract

Background: Nanotechnology is used in stem cell culture as well as in vivo delivery and tracking of stem cells. Graphene oxide (GO) is a carbon based nanomaterial and it has large surface area as well as good biocompatibility and heteroatoms doped GO exploit its properties. Hybrid GO (hGO) nano structures biocompatibility is depends on its size, dose and exposure time as well as in vitro cell models and hence, need thorough cytotoxicity studies in different species in vitro cell models. 
Methods: Caprine Wharton’s jelly derived mesenchymal stem cells (WJ-MSCs) were isolated, characterized and dose dependent (100, 50, 25, 10 and 0µg /ml) in vitro cytotoxicity of  three different hGO nano structures (phosphorus doped graphene oxide titanium oxide tubes, rods and sheets) were  analysed in caprine WJ-MSCs by studying cell cytotoxicity assays.  
Result: All three hGO nano structures were damaged cell morphology at 100 and 50 µg /ml doses, however, morphologically more good cells were observed in hGO tubes treated group than hGO rods and hGO sheets at 25 and 10 µg/ml doses as compared to control. Cell viability percentage was significantly (P<0.01) decreased at dose 100 µg/ml and it was significantly (P<0.01) increased at 25 µg/ml dose as compared to 50, 10 and 0 µg/ml doses. But, hGO tubes significantly (P<0.01) increased cell viability % as compared to hGO rods and hGO sheets. Cell population doubling time (PDT) was not altered significantly by all hGO nano structures, but 100 and 50 µg/ml doses significantly (P<0.01)increased cell PDT as compared to 25, 10 and 0 µg/ml doses. All hGO nano structures were non significantly altered growth curve, however, all hGO nano structures at 25 µg /ml dose altered (inclined) shape of growth curve, while 100 and 50 µg /ml doses significantly declined growth curve shape as compared to 10 and 0 µg /ml doses. Cell proliferation % was significantly (P<0.01) increased at 25 and 10 µg/ml doses, while, it was significantly (P<0.01) decreased at 100 µg /ml dose as compared to 50 and 0 µg /ml. However, there was no significance difference was observed in cell proliferation % in groups treated by different hGO nanostructures. In last, it was concluded as, hGO nano structures cytotoxicity was dose dependent and hGO nano tubes were least cytotoxic in caprine WJ-MSCs. 

Keywords

Caprine Graphene Nanotechnology Stem cells

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