Hepatoprotective and Nephroprotective Effect of Aegle marmelos and Andrographis paniculata in Aflatoxicosis of Broiler Chicken

DOI: 10.18805/ijar.B-3961    | Article Id: B-3961 | Page : 347-352
Citation :- Hepatoprotective and Nephroprotective Effect of Aegle marmelos and Andrographis paniculata in Aflatoxicosis of Broiler Chicken.Indian Journal Of Animal Research.2021.(55):347-352
A. Aneesh, A.J. George, B. Dhanush Krishna, M.J. Abraham, B.J. Kariyil aneeshayinippully@gmail.com
Address : College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651, Kerala, India.
Submitted Date : 3-12-2019
Accepted Date : 12-06-2020

Abstract

Background: Aflatoxins (AF) are a major threat in tropical and sub-tropical countries and causes aflatoxicosis in poultry. The deleterious effect of AF are minimised by the addition of various hepatoprotectants. Study was undertaken to find out the effect of Aegle marmelos and Andrographis paniculata on aflatoxicosis of broiler chicken.
Methods: Forty eight day old broiler chicks were divided into eight groups and fed with diets: standard feed (T1), T1 with 100 ppb AF (T2), T1 with 0.1 per cent A. marmelos powder and 0.1 per cent A. paniculata powder (T3), T1 with 100ppb AF and 0.10 per cent A. marmelos powder (T4), T1 with 100ppb AF and 0.10 per cent A. paniculata powder (T5), T1 with 100 ppb AF and 0.20 per cent A. marmelos powder (T6), T1 with 100ppb AF and 0.20 per cent A. paniculata powder (T7) and T1 containing 100 ppb AF, 0.1 per cent A. marmelos powder and 0.1 per cent A. paniculata powder (T8). All the experimental birds were sacrificed on 21st day of the experiment. 
Result: Broiler chicks of T2 group showed a significant increase in lipid peroxidation levels compared with other groups at 21st day of experiment. Liver of T2 group of birds revealed the presence of cytoplasmic vacuolations, Kupffer cell hyperplasia, focal accumulation of mononuclear cells and acinar pattern of parenchyma compared to normal histological appearance of T1 and T3 groups. Kidney of T2 birds revealed epithelial degeneration, mononuclear accumulation, desquamation of proximal convoluted tubule and vacuolation of distal convoluted tubule. The hepatoprotective and nephroprotective effect of both plant powders in aflatoxicosis was established.

Keywords

Aegle marmelos Aflatoxin Andrographis paniculata Hepatoprotective Lipid peroxidation Nephroprotective

References

  1. Abhishek, N., Tiwari, S.K. and Alok, L. (2010). Biological activities of Kalmeg (Andrographis paniculata Nees) and its active principles- a review. Indian Journal of Natural Production and Resources. 1:125-135.
  2. AL-Zuhariy, M.T. and Hassan, W.H. (2017). Hepatoprotective and immunostimulatory effect of Ganoderma, Andrographolide and Turmeric against Aflatoxicosis in broiler chickens. International Journal of Poultry Science. 16: 281-287.
  3. Aneesh, A., George, A.J., Kariyil, B.J., Krishna, D. and Abraham, M.J. (2018). Phytochemical evaluation of the leaves of Aegle marmeloes L. (L.) - an important medicinal plant. Journal of Tropical Agriculture. 56: 81-85.
  4. Aneesh, A., George, A.J., Kariyil, B.J., Krishna, D. and Sajitha, I.S. (2018). Phytochemical evaluation of Andrographis paniculata L.(L.). Journal of Pharmacognosy and Phytochemistry. 7: 1949-1953.
  5. Arulmozhi, A. (1999). Determination of permissible level of aflatoxin in broiler chicken feed. M.V.Sc. thesis, Kerala Agricultural University, Thrissur. 70p.
  6. Benjamin, M. M. (1978). Outline of Veterinary Clinical Pathology. (2nd Edn.). The Iowa State University Press, Ames Iowa. 61-69p.
  7. Bhar, K., Mondal, S. and Suresh, P. (2019). An Eye-Catching Review of Aegle marmelos L. (Golden Apple). Pharmacognosy Journal. 11: 207-224 
  8. Calnek, B.C.; Barnes, H.J. MC Dougald, L.R. and Saif, Y.M. (1997). Diseases of poultry. (10th Edn.). Mosby-Wolfe, Iowa state Univ. press, Ames, Iowa, USA, pp.951-979.
  9. Daly. (2010). Hepato-renal pathology with special reference to aflatoxicosis in chicken (Gallus domesticus). M.V.Sc. thesis, Kerala Agricultural University, Mannuthy, 78p. 
  10. Fraga, C.G., Leibovitz, B.E. and Tappel, A.L. (1988). Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radical Biology and Medicine. 4:155-161.
  11. Darwish, H.R., Omara, E.A., Abdel-Aziz, K.B., Farag, I.M., Nada, S.A. and Tawfek, N.S. (2011). Saccharomyces cerevisiae modulate aflatoxin-induced toxicity in male albino mice. Report and Opinion. 3: 32-43.
  12. George, A.J. (2007). Effect of feeding sublethal doses of aflatoxin and ochratoxin in broiler chicken. PhD. thesis, Tamilnadu Veterinary and Animal Sciences University, Chennai, 78p.
  13. Gholami-Ahangaran, M., Rangsaz, N. and Fathi-Hafshejani, E., Protective effect of turmeric on liver and kidney in chicken aflatoxicosis. International Conference on Chemical, Environmental and Biological Sciences (CEBS-2015) March 18-19, 2015 Dubai (UAE).
  14. Gholami-Ahangaran, M., Rangsaz, N. and Azizi, S. (2016). Evaluation of turmeric (Curcuma longa) effect on biochemical and pathological parameters of liver and kidney in chicken aflatoxicosis. Pharmaceutical Biology. 54: 780-787.
  15. Jakhar, K.K and Sadana, J.R. (2004). Sequential pathology of experimental aflatoxicosis in quail and the effect of selenium supplementation in modifying the disease process. Mycopathologia. 157: 99-109.
  16. Jayakumar, T., Hsieh, C.Y., Lee, J.J. and Sheu, J.R. (2013). Experimental and clinical pharmacology of Andrographis paniculata and its major bioactive phytoconstituent andrographolide. Evidence-Based Complementary and Alternative Medicin. 2013.
  17. Kashyap, G., Singh, R., Agrawal, R.K., Malik, Y.P.S., Singh, K.P., Kumar, P., Sahoo, M., Singh,R., Gupta, D. and Dar, J.A. (2017). Etiopathology of intestinal affections in bovine calves. Indian Journal of Veterinary Pathology. 41: 173-178.
  18. Kumar, C.T.A and Balachandran, C. (2014). Pathological effects of citrinin and aflatoxins in broiler chicken. International Journal of Life Science and Pharma Research. 4: 1-14.
  19. Kumar, R. and Balachandran, C. (2009). Histopathological changes in broiler chickens fed aflatoxin and cyclopiazonic acid. Veterinary Archives. 79: 31-40.
  20. Lakkawar, A.W. (2015). Pathomorphological and immuno suppressive studies on induced aflatoxicosis and its amelioration using diatomaceous earth (dae) in broiler chicken, Doctoral dissertation, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar. 288p.
  21. Neogy, S., Das, S., Mahapatra, S.K., Mandal, N. and Roy, S. (2008). Amelioratory effect of Andrographis paniculata Nees on liver, kidney, heart, lung and spleen during nicotine induced oxidative stress. Environmental Toxicology and Pharmacology. 25: 321-328.
  22. Nithya. (2016). Evaluation of mast cell response in caecal coccidiosis of broiler chicken in sub-lethal aflatoxicosis. M.V.Sc. thesis, Kerala Veterinary and Animal Sciences University, Pookode, 70p.
  23. Ortatatli, M., Oðuz, H., Hatipoðlu, F. and Karaman, M. (2005). Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Research in. Veterinary Sciences. 78: 61-68.
  24. Ramdas, R.P., Balkrishna, K.G. and Govind, G. (2013). Pathological effect of low grade aflatoxicity in broilers. The Bioscan. 8: 1115-1118.
  25. Seth, E., Vijay Lakshmi Sharma. and Chopra, M. (2016). Studies on ameliorative effect of Aegle marmelos on cadmium induced splenic toxicity in murine model. European Journal of Biomedical and Pharmaceutical Sciences. 3: 282-287
  26. Shakya, A.K. (2020). Drug-induced Hepatotoxicity and Hepatoprotective Medicinal Plants: A Review. Indian Journal of Pharmaceutical Education and Research. 54: 234-250.
  27. Shotwell, O.L., Hesseltine, C.W., Stubblefield, R.D. and Sorenson, W.G. (1966). Production of aflatoxin on rice. Applied Microbiology. 14: 425-428.
  28. Singh, D.P., Awasthi, H., Luqman, S., Singh, S. and Mani, D. (2015). Hepatoprotective effect of a polyherbal extract containing Andrographis paniculata, Tinospora cordifolia and Solanum nigrum against paracetamol induced hepatotoxicity. Pharmacognosy Magazine. 11: 375.
  29. Sivakumar, V. and Rajeshkumar, S. (2015). Protective effect of Andrographis paniculata on hyperglycemic mediated oxidative damage in renal tissues of diabetic rats. Journal of Phytopharmacology. 4: 287-294.
  30. Suvarna, K.S., Layton, C. and Bancroft, J.D. 2018. Bancroft’s Theory and Practice of Histological Techniques E-Book (8th Edn.). Elsevier Health Sciences.
  31. Verma, R.J. and Mathuria, N. (2008). Curcumin ameliorates aflatoxin-induced lipid-peroxidation in liver and kidneyof mice. Acta Poloniae Pharmaceutica. 65:195-202.
  32. Yarru, L.P., Settivari, R.S., Gowda, N.K.S., Antoniou, E., Ledoux, D.R. and Rottinghaus, G.E. (2009). Effects of turmeric (Curcumalonga) on the expression of hepatic genes associated with biotransformation, antioxidant, and immune systems in broiler chicks fed aflatoxin. Poultry Science. 88: 2620-2627.
  33. Yavuz, O., Özdemir, Ö., Ortatatli, M., Atalay, B., Hatipoglu, F. and Terzi, F. (2017). The preventive effects of different doses of glucomannan on experimental aflatoxicosis in Japanese quails. Revista Brasileira de Ciência Avícola. 19: 409-416.

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