Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 10 (october 2020) : 1229-1234

Comparative Study on Spermatological Parameters and Seminal Plasma Composition of Labeo rohita Strains from Tamil Nadu, India

M. Muthu Abishag, C. Judith Betsy, J. Stephen Sampath Kumar
1Department of Aquaculture, Fisheries College and Research Institute, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Thoothukudi-628 008, Tamil Nadu, India.
Cite article:- Abishag Muthu M., Betsy Judith C., Kumar Sampath Stephen J. (2020). Comparative Study on Spermatological Parameters and Seminal Plasma Composition of Labeo rohita Strains from Tamil Nadu, India. Indian Journal of Animal Research. 54(10): 1229-1234. doi: 10.18805/ijar.B-3879.

ABSTRACT

Background: The fertilization success and seed quality of Labeo rohita from different locations show wide variation. The important reason for these variations is sperm quality. Spermatological parameters are considered to be the deciding factors of sperm quality. The present study envisages delineating the strain-specific variations in spermatological parameters and seminal plasma composition of L. rohita from three different locations (Manimuthar, Thanjavur and Mettur) of Tamil Nadu. 
Methods: In the present study conducted during 2019, milt samples were collected from three different strains of Labeo rohita. The samples were analyzed for the spermatological parameters such as pH, volume, sperm density, spermatozoa motility and seminal plasma composition (Na+, K+, Ca2+, Mg2+, Cl-) following standard protocols and the statistical comparisons were made.
Result: Among the strains, the spermatozoa of Thanjavur strain exhibited best performance with highest motility duration (82.33±6.03 s) (p<0.025) and sperm density (2.526±0.25 × 109 cells/ml). With regard to seminal plasma composition, the concentration of Na+ (113.64±4.97 mmol/L) and K+ (149.91±1.67 mmol/L) was the highest in Thanjavur strain. Mettur strain exhibited highest Ca2+ (1.24±0.06 mmol/L) content (p<0.01) besides its lowest Na+ concentration (51.28±3.52 mmol/L). The results revealed a significant correlation between ionic composition of seminal plasma and sperm motility. The increased motility duration in Thanjavur strain was due to increased K+ concentration. A synergistic effect of ions in controlling sperm motility was also observed as the higher Ca2+ concentration could sustain lowered Na+ concentration in Mettur strain.

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