Loading...

Indian Journal of Animal Research

Cryopreservation and fertility of frozen thawed Chegu goat semen

DOI: 10.18805/ijar.B-3696    | Article Id: B-3696 | Page : 1414-1419
Citation :- Cryopreservation and fertility of frozen thawed Chegu goat semen.Indian Journal of Animal Research.2019.(53):1414-1419
A. Sharma and P. Sood vet50amy@gmail.com
Address : Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalya, Palampur-176 062, Himachal Pradesh, India.
Submitted Date : 25-07-2018
Accepted Date : 22-01-2019
First Online:

Abstract

Goats are important livestock species of India. Chegu is a pashmina producing goat native to the cold arid region of Himachal Pradesh (H.P.), India. Semen cryopreservation from six Chegu elite males (aged 2.05±0.40 years; weighed 29.16±2.02 kg) was practiced using Tris Citrate Egg Yolk extender containing 10% EY and 6% Glycerol. Gross semen parameters includes volume (0.80.85±0.07 ml), color (Creamy white to yellowish), concentration (2238.5±231.0 x106 spermatozoa/ml) and mass motility (3.92±0.03).The significant changes (P<0.01) in post thaw seminal parameters (75.48±0.69 v/s 37.38±0.90; progressive motility), viability (75.79±0.95 v/s 48.25±1.78), morphological abnormalities (5.64±0.29 v/s 7.02±0.32) and HOST reactive spermatozoa (64.07±1.75 v/s 43.35±1.79) were observed in present study. Artificial insemination using frozen thawed semen having concentration (150 x106 spermatozoa/straw) from three different bucks was practiced in 40 synchronized goats with conception rate of 42.5 per cent. Non-significant variations amongst different bucks were observed with birth of 1.12 kids per doe and twinning rate of 11.8 per cent. It was concluded that semen cryopreservation along with artificial insemination can be practiced in Chegu goats to improve the population of this endangered species. 

Keywords

Artificial insemination Chegu goat Fertility Semen Seminal plasma.

References

  1. Arav, A., Michal, P., Zeron, Y. (2000). Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocyte? Cryoletters, 21: 179-186. 
  2. Bag, S., Joshi, A., Rawat, P.S., Mittal, J.P. (2002). Effect of initial freezing temperature on the semen characteristics of frozen thawed ram spermatozoa in a semi-arid tropical environment. Small Ruminant Research, 43: 23–29.
  3. Batista, M., Nino, T., Alamo, D., Castro, N., Santana, M., Gonzalez, F., Cabrera, F., Gracia, A. (2009). Successful artificial insemination using semen frozen and stored by an ultrafreezer in the Majorera goat breed. Theriogenology, 71: 1307- 1315.
  4. Bhattacharyya, H.K., Ganai, N.A., Khan, H.M. (2012). Fertility of local goats of Kashmir using frozen semen of Boer bucks. Wudpecker Journal of Agricultural Research, 8: 346–348.
  5. Bloom, E. (1977). Sperm morphology with reference to bull infertility. In: Some papers contributed to the 1st All India Symposium on Animal Reproduction, Punjab Agricultural University, Ludhiana, India, pp. 66-81. 
  6. Chahota, R., Gupta, S., Bhardwaj, B., Malik, P., Verma, S., Sharma, M. (2015). Seroprevalence studies on animal Chlamydiosis amongst ruminants I five states of India. Veterinary World, 8(1): 72-75. 
  7. Dogra, P.K., Thakur, Y.P. (2010). Livestock biodiversity of Himachal Pradesh with special reference to pashmina goats. In: Proceeding of National training on “Production, processing and utilization of pashmina fibre” 1-7 Dec Palampur, H.P, India pp 1-12. 
  8. Dorado, J., Munoz-Serrano, ˜A., Hidalgo, M. (2010). The effect of cryopreservation on goat semen characteristics related to sperm freezability. Animal Reproduction Science, 121: 115–123.
  9. Dorado, J., Rodríguez, I., Hidalgo, M. (2007). Cryopreservation of goat spermatozoa: comparison of two freezing extenders based on post- thaw sperm quality and fertility rates after artificial insemination. Theriogenology, 68: 168–177.
  10. Drobnis, E.S., Crowe, L.M., Berger, T., Anchodoguy, T.J., Overstreet, J.W., Crowe, J.H. (1993). Cold shock damage is due to lipid phase transition in cell membranes: a demonstration using sperm as a model. Journal of Experiment Zoology, 265(4): 432-437. 
  11. Elsheikh, A.S., Elhammali, N.S. (2015). Semen quality of mature crossbred male goats during different seasons. IOSR-Journal of Agriculture and Veterinary Science, 8(9):1-5.
  12. Gallego-Calvo, L., Carolina, M., Gatica, Moreno, J.S., José, L.G., Zarazaga, L.A. (2015). Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks. Spanish Journal of Agricultural Research, 13(4): 1-10.
  13. Gangwar, C., Kharche, S.D., Kumar, S., Jindal, S.K. (2016). Cryopreservation of goat semen: status and prospects. Indian Journal of Small Ruminant, 22: 1–10.
  14. Hafez, B., Hafez, E.S.E. (2000). Reproduction in Farm Animals. 7th ed. Lippincott Williams and Wilkens, New York. pp. 509.
  15. Hannock, J.L. (1951). A staining technique for study of temperature shock in semen. Nature, 167: 323-324.
  16. ICAR. (2013). Nutrient Requirements of Sheep, Goats and Rabbits. 3rd ed. ICAR, New Delhi.
  17. Jimenez-Rabadan, P., Ramon, M., Garcia-Alvarez, O., Maroto-Morales, A., et al (2013). Improved cryopreservation protocol for Blanca-Celtiberica buck semen collected by electroejaculation. Cryobiology, 67: 251–257.
  18. Kharche, S.D., Jindal, S.K., Priyadarshini, R., Kumar, S., Goel, et al (2013). Fertility following frozen semen artificial insemination in Jamunapari goats. Indian Journal of Animal Sciences, 83(10): 1071-1073.
  19. Kumar, N., Rai, B., Bhat, S.A., Kharche, S.D., Gangwar, C., Jindal, S.K., Chandra, S. (2016). Effect of management system and season on semen freezability in Jakhrana bucks. Veterinary World, 9(2): 199-202.
  20. Leboeuf, B., Restall, B., Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science, 62: 113–141.
  21. Mohan, O., Mazumder, N.K., Gooswami, N.K. (1980). Note on semen characteristics in Indian Pashmina goats. Indian Journal of Animal Sciences, 50: 898-900. 
  22. Narwade, B.M., Mohanty, T.K., Bhakat, M., Rahim, A. (2017). Goat semen cryopreservation using egg yolk and soya based extenders containing trehalose. Indian Journal of Animal Sciences, 87(7): 851-855. 
  23. Neild, D.M., Brouwers, J.P., Colenbrander, B., Aguero, A., Gadella, B.M. (2005). Lipid peroxide formation in relation to membrane stability of fresh and frozen-thawed stallion spermatozoa. Molecular Reproduction and Development, 72: 230-238.
  24. Niyazi, K., Melih, A., Ugar, U., Ejaz, A., Zahid, Ahmet, C., Ilker, S. (2014). Comparison of two different cryopreservation protocols for freezing goat semen. Cryobiology, 68: 327-331. 
  25. Nordstoga, A.B., Soderquist, L., Adnoy, T., Farstad, W., Paulenz, H. (2010). Vaginal deposition of frozen-thawed semen in Norwegian Dairy goats: comparison of single and double insemination with equal total number of spermatozoa. Theriogenology, 74: 895-900.
  26. Nuti, I. (2007). Techniques of artificial insemination in goats. In: Current Therapy in Large Animal Theriogenology. [Youngquist, R.S. and Threlfall, W.R (eds)] 2nd ed. Sounders-Elsevier, St. Louis, MO. pp. 529-534. 
  27. OIE Quality Standards and Guidelines for Veterinary Laboratories, 2nd ed., 2008, OIE, Paris. 
  28. Ozkavukcu, S., Erdemli, E., Isik, A., Oztuna, D., Kara-huseyinoglu, S. (2008). Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. Journal of Assisted Reproduction and Genetics, 25: 403-411.
  29. Pant, H.C., Barot, L.R., Kasiraj, R., Misra, A.K., Prabhakar, J.H. (2002). Acrosomal integrity of buffalo spermatozoa and its relation to fertility. Indian Journal of Animal Reproduction, 23: 110-112.
  30. Pierson, J.T., Baldassarre, H., Keefer, C.L., Downey, B.R. (2003). Influence of GnRH administration on the timing of the LH surge and ovulation in dwarf goats. Theriogenology, 60: 397-406. 
  31. Priyadharsini, R., Jindal, S.K., Sharma, D., Ramachandran, N., Kharche, S.D., Goel, A.K. (2011). Effect of different egg yolk level on the cryopreservation capability of Jakhrana goat semen. Journal of Animal Science Advances, 1(1): 28-37.
  32. R Core Team. (2013). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 
  33. Ramachandran, N., Yadav, S., Sikarwar, A.K.S., Saraswat, S., Ranjan, R., Jindal, S.K. (2015). Effect of equilibration periods on post-thaw semen quality of Jamunapari bucks. Indian Journal of Small Ruminant, 21(2): 234-237.
  34. Ranjan, R., Goel, A.K., Ramchandran, N., Kharche, S.D., Jindal, S.K. (2015). Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian Journal of Small Ruminant, 21(1): 32-36.
  35. Rather, H.A., Islam, R., Malik, A.A., Lone, F.A. (2016). Addition of antioxidants improves quality of ram spermatozoa during preservation at 40C. Small Ruminant Research 141: 24-28.
  36. Salvador, I., Viudes-de-Castro, M.P., Bernacer, J., Gomez, E.A., Silvestre, M.A. (2005). Factors affecting pregnancy rate in artificial insemination with frozen semen during non-breeding season in Murciano-Granadina goats: a field assay. Reproduction in Domestic Animals, 40: 526-529.
  37. Sariözkan, S., Bucak, M.N., Tuncer, P.B., Taºdemir, U., Kinet, H., Ulutaº, P.A. (2010). Effects of different extenders and centrifugation/    washing on post thaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm. Theriogenology 73: 316-323. 
  38. Sharma, A.K. (2018). Investigations on follicular dynamics and semen cryopreservation in goats. PhD Thesis CSKHPKV Palampur, Himachal Pradesh, India.
  39. Singh, L.G., Ray, K., Sarkar, B. (2016). Effect of different levels of egg yolk on cryopreservation of Black Bengal buck semen in tris egg yolk citrate fructose glycerol extender. Iranian Journal of Applied Animal Science, 6: 101–106.
  40. Stanimir, A.Y., Desislava, V.V., Lora, R.D. (2016). Pregnancy rate in Bulgarian White milk goats with natural and synchronized estrus after artificial insemination by frozen semen during breeding season. Asian Pacific Journal of Reproduction, 5(2): 144-147. 
  41. Tabarez, A., Garcia, W., Palomo, M.J. (2017). Effect of the type of egg yolk, removal of seminal plasma and donor age on buck sperm cryopreservation. Small Ruminant Research, 149: 91-98.
  42. Thakur, Y.P., Katoch, S., Dogra, P.K. (2005a). Production system and demographic status of Chegu goats in their breeding tract in Himachal Pradesh. Indian Journal of Small Ruminant, 11(2): 116-120. 
  43. Thakur, Y.P., Singh, M., Jasial, S. (2005b). Semen production and freezability attributes of Chegu Pashmina bucks. Indian Journal of Animal Sciences, 75: 1165-1167.
  44. Tiwari, S.B., Bhattacharya, N.K. (1988). Post thaw motility of frozen semen and conception rate in Jamunapari and Barbari goat. VII Annual Convention of Indian Society of Study on Animal Reproduction, Mannuthy, Kerela. August 22-24.
  45. Viudes-De-Castro, M.P., Salvador, I., Marco-Jiménez, F., Gómez, E.A., Silvestre, M.A. (2009). Effect of oxytocin treatment on artificial insemination with frozen-thawed semen in Murciano-Granadina goats. Reproduction in Domestic Animals, 44: 576–579.
  46. Waidi, F.S., Matthew, O.O., Matthew, O.A. (2007). Coconut milk-Citrate as extender for WAD buck spermatozoa at room temperature. Biokemistri 19(2): 65-73. www.bioline.org.br/request/bk0711 Vol.19 No.2.
  47. Yodmingkwan, P., Guntaprom, S., Jaksamrit, J., Lertchunhakiat, K. (2016). Effects of extenders on fresh and freezing of Boer goat. Agricultural Science Procedia. 11: 125–130. 

Global Footprints