Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 7 (july 2019) : 870-873

Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c

B. Singh, K.K. Bonia, M.K. Tamuli, S. Sinha, S. Tamuly, A.K. Sarma, G. Bhuyan
1Assam Agricultual University, Khanapara, Guwahati-781 022, Assam, India.
Cite article:- Singh B., Bonia K.K., Tamuli M.K., Sinha S., Tamuly S., Sarma A.K., Bhuyan G. (2018). Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c. Indian Journal of Animal Research. 53(7): 870-873. doi: 10.18805/ijar.B-3603.

ABSTRACT

A total of  30 ejaculates comprising five from each  of six boars (three Hampshire and three Rani breeds) were collected to study the effect of  DMSO (Dimethyl sulfoxide) on preservability  and fertility  of  boar semen at liquid state at 15°C in ten extenders viz.Androheps+Strepto-penicillin+Gentamycin GEPS+Strepto-penicillin+Gentamycin, Androheps + 1% DMSO, Androheps + 0.7% DMSO, Androheps + Gentamicin sulfate + 0.5% DMSO,Androheps +Strepto-penicillin + 0.5% DMSO, GEPS (Glucose-sodium salt of EDTA-potassium sodium tartrate-sodium citrate dihydrate) + 1% DMSO, GEPS+0.7% DMSO, GEPS+Gentamicin sulfate+0.5% DMSO and GEPS+ Strepto-penicillin + 0.5% DMSO extenders. After holding semen each ejaculate was split into 10 parts and extended (1:3) with the said experimental dilutors and preserved at 15°C in refrigerator up to 168 hr. The evaluation of sperm motility and Hypo-osmotic swelling test (HOST) of preserved semen samples were recorded at 0 (just after extension), 24, 48, 72, 96, 120, 144 and 168 hr as per the standard method. The results indicated that the addition of DMSO in extenders had no significant effect on preservability of boar semen at 15°C.

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