In vitro selection of potential immunomodulatory multispecies lactic acid bacteria in broilers

DOI: 10.18805/ijar.B-3547    | Article Id: B-3547 | Page : 655-660
Citation :- In vitro selection of potential immunomodulatory multispecies lactic acid bacteria in broilers.Indian Journal Of Animal Research.2019.(53):655-660
K. Divya Manjari, G. Divya, T.S. Saravanan and M. Parthiban manjubrindha@gmail.com
Address : Department of Animal Biotechnology, Madras Veterinary College, Faculty of Basic Sciences, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.
Submitted Date : 23-12-2017
Accepted Date : 3-04-2018

Abstract

Modulation of host immunity is one of the proposed benefits of the consumption of probiotics.  In this study a total of 26 lactic acid bacteria belonging to eight different species were isolated from chicken gut. These twenty six probiotic organisms were characterized by Gram’s staining, biochemical methods using 21 sugars, pH tolerance and bile salt tolerance test.  These probiotic organisms were identified using PCR amplification of 16S-23S spacer region and sequencing analysis. Based on the sequencing data, 6 different species were found to exist namely Enterococcus faecalis; Enterococcus faecium, Enterococcus hirae, Weissella cibaria, Lactobacillus salivarius, Pediococcus acidilactici. To select the immunomodulatory probiotic bacteria, the scoring index was established based on different chicken cytokine expression and B and T cell receptor expression studies. According to scoring index, Pediococcus acidilactici, Lactobacillus salivarius and Weissellacibaria showed highest levels of fold increase in the expression of Th1 cytokines viz. IL2, IL 12p40, IL1b and TNF a. Enterococcus faecalis and Enterococcus faecium showed highest levels of fold increase in the expression of Th2 cytokines viz. IL 4, IL10, TGF b and IL6.

Keywords

Broilers Cytokines Flow Cytometry Immunomodulation Probiotic Real Time PCR.

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