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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 3 (march 2019) : 321-326,   Doi: 10.18805/ijar.B-3505

Effect of feeding synbiotic products on the serum cholesterol, HDL cholesterol and non- HDL cholesterol level of albino rats

R
Rakesh Kumar
B
Binita Rani
S
Sonia Kumari
A
A.K. Jha
1Sanjay Gandhi Institute of Dairy Technology, Patna- 800014, Bihar, India.
Cite article:- Kumar Rakesh, Rani Binita, Kumari Sonia, Jha A.K. (2018). Effect of feeding synbiotic products on the serum cholesterol, HDL cholesterol and non- HDL cholesterol level of albino rats. Indian Journal of Animal Research. 53(3): 321-326. doi: 10.18805/ijar.B-3505.

ABSTRACT

Considering the excellent bio-therapeutic benefits of Lactobacillus and Bifidobacterium, this study exploited the probiotic characteristics of Lactobacillus acidophilus-015, Lactobacillus casei-297 and Bifidobacterium bifidum-229 strains with natural prebiotic substances viz. banana powder, malto-dextrin and honey to produce synbiotic food formulations and to study the effect of feeding their on the cholesterol level (mg/dl) of albino rats. On the basis of the preliminary investigation, synbiotic products with 2% banana powder, with 2% malto-dextrin, with 3% honey and synbiotic product with 2% each of, banana powder and malto-dextrin and 1% of honey were selected for the investigation of the total cholesterol, HDL-cholesterol and non-HDL cholesterol. The products showed a significant decrease in total cholesterol level, however the control group receiving the cholesterol rich laboratory diet showed a significant increase (P<0.01) in the total cholesterol after 45 days of feeding. There was 25.81 to 45.05% reduction of serum cholesterol was observed in case of feeding synbiotic formulations. Increase in the HDL-cholesterol and decrease in the non-HDL cholesterol were also significant.  Therefore, synbiotic formulation with all these three natural prebiotic have enhanced ability to decreased down the blood cholesterol level. 

REFERENCES

  1. Adebawo, O.O., Banjoko, Y. and Osilesi,T. (2008). Evaluation of the hypochol-esterolemic effect of Lactobacillus acidophilus fermented maize meal (ogi) in rats. The FASEB Journal 22: 1092-1097.
  2. Anand, S.K., Srinivasan, R.A. and Rao, L.K. (1984). Antimicrobial activity associated with Bifidobacterium bifidum. Cultured Dairy Product Journal 20: 21-23 . 
  3. Begley,M., Hill, C., Gahan,C.G.M (2006) Bile salt hydrolase activity in probiotics. Appllied. Environmantal. Microbiology. 72: 1729–1738.
  4. Davis, J.G. (1955) Dictionary of Dairying 2nd Ed..Leonard Hill Ltd. London pp. 582.
  5. Eyssen, H. (1973) Role of gut microflora in metabolism of lipids and sterols. Proceding of Nutrition Society 32: 59–63.
  6. Gilliland, S.E., Nelson ,C.R..and Maxwell, C. (1985) Assimilation of cholesterol by Lactobacillus acidophilus. Applied Environmental Microbiology 49: 377–381.
  7. Kawase, M., Hashimoto, H., Hosoda, M., Morita, H. and Hosona, A. (2000). Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. Journal of Dairy Science 83: 255-263
  8. Liong,M.T.,and Shah,N.P. (2005a). Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science 88:55–66. 
  9. Liong, M.T.and Shah, N.P. (2005b). Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. International Dairy Journal 15: 391–398. 
  10. Liong, M.T. and Shah,N.P. (2006).Effects of a Lactobacillus casei synbiotic on serum lipoprotein, intestinal microflora and organic acids in rats. Journal of Dairy Science 89:1390-1399.
  11. Mann, G.V., Schaffer, R.D., Anderson, R.D, and Sand-Stead, H.H. (1964). Cardiovascular disease in the Massai. Journal of Aetheroscler Research 4: 289-312
  12. Montgomery, D.C. (1991).Experiments with a single factor: the analysis of variance. In Design and Analysis of Experiments (DC Montgomery, editor) NY: Jone Wiley and sons, pp75-77 New York,. 
  13. Oyetayo, V.O., Adetuyi, F.C. and Akinyosoye, F.A. (2003) Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo. African Journal of Biotechnology 2: 448-452.
  14. Sanders, M.E. (1994) Lactic acid bacteria as promoters of human health. In: “Functional Foods-Designer Foods, Pharmafoods, Nutraceuticals” ed. I. Goldberg, Chapman & Hall, N.Y pp. 294-322. 
  15. Sahin, T, Kaya, I, Unal, Y.and Emali, D.A. (2008) Dietary supplementation of probiotics and prebiotics combination on performance, carcass quality and blood parameters in growing quail. Journal of Animal and Veterinary 7: 1370-1373.
  16. Schafsma, G, Meuling, W.J.A., Dokkum, W, and Bouley, C. (1998). Effect of a milk product, fermented by Lactobacillus acidophilus with fructo-oligosaccharides added, on blood lipids in male volunteers. European Journal of Clinical Nutrition 52: 436–440.
  17. Shovna, Suja, S. and Prajapati, J. B. (2013). Food allergy: Its control by probiotic - A review. Asian J. Dairy & Food Re
  18. Taylor G.R and Williams, C.M.(1998) Effects of probiotics and prebiotics on blood lipids. Brit J Nutr 80: S225–S230.
  19. Wybenga, D.R., and Pileggi V.J. (1970). One step method for the in vitro determination of cholesterol in serum / plasma. Journal of Clinical Chemistry 16: 980. 
  20. Xiao, J.Z., Kondo, S. Takahashi, N, Miyaji, K. Oshida, K. Hiramatsu, A. Iwatsuki, K. Kokubo, S.and Hosono, A. (2003) Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. Journal of Dairy science 86: 2452-2461.
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 3 (march 2019) : 321-326,   Doi: 10.18805/ijar.B-3505

    Effect of feeding synbiotic products on the serum cholesterol, HDL cholesterol and non- HDL cholesterol level of albino rats

    R
    Rakesh Kumar
    B
    Binita Rani
    S
    Sonia Kumari
    A
    A.K. Jha
    1Sanjay Gandhi Institute of Dairy Technology, Patna- 800014, Bihar, India.
    Cite article:- Kumar Rakesh, Rani Binita, Kumari Sonia, Jha A.K. (2018). Effect of feeding synbiotic products on the serum cholesterol, HDL cholesterol and non- HDL cholesterol level of albino rats. Indian Journal of Animal Research. 53(3): 321-326. doi: 10.18805/ijar.B-3505.

    ABSTRACT

    Considering the excellent bio-therapeutic benefits of Lactobacillus and Bifidobacterium, this study exploited the probiotic characteristics of Lactobacillus acidophilus-015, Lactobacillus casei-297 and Bifidobacterium bifidum-229 strains with natural prebiotic substances viz. banana powder, malto-dextrin and honey to produce synbiotic food formulations and to study the effect of feeding their on the cholesterol level (mg/dl) of albino rats. On the basis of the preliminary investigation, synbiotic products with 2% banana powder, with 2% malto-dextrin, with 3% honey and synbiotic product with 2% each of, banana powder and malto-dextrin and 1% of honey were selected for the investigation of the total cholesterol, HDL-cholesterol and non-HDL cholesterol. The products showed a significant decrease in total cholesterol level, however the control group receiving the cholesterol rich laboratory diet showed a significant increase (P<0.01) in the total cholesterol after 45 days of feeding. There was 25.81 to 45.05% reduction of serum cholesterol was observed in case of feeding synbiotic formulations. Increase in the HDL-cholesterol and decrease in the non-HDL cholesterol were also significant.  Therefore, synbiotic formulation with all these three natural prebiotic have enhanced ability to decreased down the blood cholesterol level. 

    REFERENCES

    1. Adebawo, O.O., Banjoko, Y. and Osilesi,T. (2008). Evaluation of the hypochol-esterolemic effect of Lactobacillus acidophilus fermented maize meal (ogi) in rats. The FASEB Journal 22: 1092-1097.
    2. Anand, S.K., Srinivasan, R.A. and Rao, L.K. (1984). Antimicrobial activity associated with Bifidobacterium bifidum. Cultured Dairy Product Journal 20: 21-23 . 
    3. Begley,M., Hill, C., Gahan,C.G.M (2006) Bile salt hydrolase activity in probiotics. Appllied. Environmantal. Microbiology. 72: 1729–1738.
    4. Davis, J.G. (1955) Dictionary of Dairying 2nd Ed..Leonard Hill Ltd. London pp. 582.
    5. Eyssen, H. (1973) Role of gut microflora in metabolism of lipids and sterols. Proceding of Nutrition Society 32: 59–63.
    6. Gilliland, S.E., Nelson ,C.R..and Maxwell, C. (1985) Assimilation of cholesterol by Lactobacillus acidophilus. Applied Environmental Microbiology 49: 377–381.
    7. Kawase, M., Hashimoto, H., Hosoda, M., Morita, H. and Hosona, A. (2000). Effect of administration of fermented milk containing whey protein concentrate to rats and healthy men on serum lipids and blood pressure. Journal of Dairy Science 83: 255-263
    8. Liong,M.T.,and Shah,N.P. (2005a). Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science 88:55–66. 
    9. Liong, M.T.and Shah, N.P. (2005b). Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. International Dairy Journal 15: 391–398. 
    10. Liong, M.T. and Shah,N.P. (2006).Effects of a Lactobacillus casei synbiotic on serum lipoprotein, intestinal microflora and organic acids in rats. Journal of Dairy Science 89:1390-1399.
    11. Mann, G.V., Schaffer, R.D., Anderson, R.D, and Sand-Stead, H.H. (1964). Cardiovascular disease in the Massai. Journal of Aetheroscler Research 4: 289-312
    12. Montgomery, D.C. (1991).Experiments with a single factor: the analysis of variance. In Design and Analysis of Experiments (DC Montgomery, editor) NY: Jone Wiley and sons, pp75-77 New York,. 
    13. Oyetayo, V.O., Adetuyi, F.C. and Akinyosoye, F.A. (2003) Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo. African Journal of Biotechnology 2: 448-452.
    14. Sanders, M.E. (1994) Lactic acid bacteria as promoters of human health. In: “Functional Foods-Designer Foods, Pharmafoods, Nutraceuticals” ed. I. Goldberg, Chapman & Hall, N.Y pp. 294-322. 
    15. Sahin, T, Kaya, I, Unal, Y.and Emali, D.A. (2008) Dietary supplementation of probiotics and prebiotics combination on performance, carcass quality and blood parameters in growing quail. Journal of Animal and Veterinary 7: 1370-1373.
    16. Schafsma, G, Meuling, W.J.A., Dokkum, W, and Bouley, C. (1998). Effect of a milk product, fermented by Lactobacillus acidophilus with fructo-oligosaccharides added, on blood lipids in male volunteers. European Journal of Clinical Nutrition 52: 436–440.
    17. Shovna, Suja, S. and Prajapati, J. B. (2013). Food allergy: Its control by probiotic - A review. Asian J. Dairy & Food Re
    18. Taylor G.R and Williams, C.M.(1998) Effects of probiotics and prebiotics on blood lipids. Brit J Nutr 80: S225–S230.
    19. Wybenga, D.R., and Pileggi V.J. (1970). One step method for the in vitro determination of cholesterol in serum / plasma. Journal of Clinical Chemistry 16: 980. 
    20. Xiao, J.Z., Kondo, S. Takahashi, N, Miyaji, K. Oshida, K. Hiramatsu, A. Iwatsuki, K. Kokubo, S.and Hosono, A. (2003) Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. Journal of Dairy science 86: 2452-2461.
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