Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 53 issue 1 (january 2019) : 41-44

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

G. Kadirvel, M. K. Kalita, Raju Kr. Dewry, Ashok Kumar, Nripendra Mahanta, L. Anandakumar Singh, S.K. Baishya, S.V. Ngachan
1Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam-793 103, Meghalaya, India.
Cite article:- Kadirvel G., Kalita K. M., Dewry Kr. Raju, Kumar Ashok, Mahanta Nripendra, Singh Anandakumar L., Baishya S.K., Ngachan S.V. (2018). Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India. Indian Journal of Animal Research. 53(1): 41-44. doi: 10.18805/ijar.B-3464.

ABSTRACT

Study was conducted to compare the semen quality and fertility of liquid stored semen for three days and frozen-thawed semen in the north-eastern region of India. For liquid semen, the semen ejaculates were extended in Beltsville Thawing Solution (BTS) extender and preserved at 17°C for three days. For cryopreservation, semen was diluted Lactose-egg yolk-glycerol extender and frozen in straw using programmable freezer with freezing rate of 40°C/min from -6 to -140°C. The preserved evaluated for sperm motility, viability, plasma membrane integrity and fertility. The results revealed that the liquid stored semen has maintained the sperm motility and viability up to day 3 without significant reduction. Similarly the plasma membrane integrity did not differ significantly up to day 2, but it was significantly (P<0.05) reduced on days 3 in liquid stored semen. After freezing and thawing, the mean sperm motility, viability and plasma membrane integrity were 58.25 ± 2.96%, 64.75 ± 2.47% and 47.06 ± 2.02%, respectively. These parameters were significantly (P<0.01) lower as compared to the liquid stored semen from day 0 to day 3. After insemination with liquid semen, the farrowing rate was 77.7%, 80.76%, 73.07% and 69.8%, respectively from day 0, day1, day 2 and day 3. The pregnancy rate, farrowing rate and litter size did not differ significantly among different days of liquid storage. These parameters were significantly (P<0.01) lower in frozen semen as compare to that of liquid stored semen. The study concluded that the liquid semen stored up to three days is more efficient than frozen-thawed semen in terms of preserving sperm quality and fertility.

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