Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 12 (december 2018) : 1739-1744

Isolation and screening of Lactobacillus species from dogs for probiotic action
 

N. Mohammad Sharif, B. Sreedevi, R.K. Chaitanya, D. Sreenivasulu
1Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India.
Cite article:- Sharif Mohammad N., Sreedevi B., Chaitanya R.K., Sreenivasulu D. (2017). Isolation and screening of Lactobacillus species from dogs for probiotic action. Indian Journal of Animal Research. 52(12): 1739-1744. doi: 10.18805/ijar.B-3429.

ABSTRACT

The present study aimed at the detection of suitable Lactobacillus species from dogs for usage as probiotic. A total of 67 rectal swabs from healthy pups were analyzed and 49 (73.1%) Lactobacillus isolates were identified based on morphological, biochemical characteristics and confirmed by genus specific PCR targeting 16S rRNA gene. A total of 20 isolates that showed strong aggregation, high cell surface hydrophobicity, acid and bile tolerance were screened for in-vitro antibacterial activity by agar well diffusion assay, where prominent inhibitory zones were observed against majority of the test pathogens. Reduction in antibacterial activity was noticed after neutralization, proteinase K and heat treatment of supernatants. Partial 16S rRNA nucleotide sequence analysis of seven Lactobacillus isolates that showed prominent in-vitro antibacterial activity revealed maximum sequence homology with Lactobacillus fermentum (for six isolates) and Lactobacillus agilis (for one isolate). The in-vitro antibacterial activity results highlighted the need for in-vivo studies in India to establish probiotic potential of canine faecal Lactobacillus species in the near future.

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