Banner

Indian Journal of Animal Research

  • Chief EditorM. R. Saseendranath

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.40

  • SJR 0.233, CiteScore: 0.606

  • Impact Factor 0.5 (2025)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 52 issue 10 (october 2018) : 1433-1439

Probiotic and prebiotic supplementation improving the production performance and immune characteristics of laying hens

Nancy Sheoran, Sushila Maan, Aman Kumar, Kanisht Batra, Deepika Chaudhary, Sajjan Sihag, Vinay Kumar, N.S.Maan
1Department of Animal Nutrition, College of Veterinary Sciences, LLR University of Veterinary and Animal Sciences, Hisar-125 004, Haryana, India.
Cite article:- Sheoran Nancy, Maan Sushila, Kumar Aman, Batra Kanisht, Chaudhary Deepika, Sihag Sajjan, Kumar Vinay, N.S.Maan (2017). Probiotic and prebiotic supplementation improving the production performance and immune characteristics of laying hens. Indian Journal of Animal Research. 52(10): 1433-1439. doi: 10.18805/ijar.B-3394.

ABSTRACT

A 16 week feeding trial was conducted with 140 White Leghorn layers of 22-23±0.08 weeks of age which were distributed randomly into seven experimental groups, having 4 replicates with 5 birds in each. The laying hens of control group (T1) were fed a basal diet (CP 18.04%), while in treatment groups T2, T3, T4 basal diet was supplemented with probiotic (containing 5×108 cfu/g of Lactobacillus fermentum, 1×109 cfu/g of Bacillus spp., 1×109cfu/g of Saccharomyces cerevisiae) at levels of 0.5g, 1.0 g and 2.0 g /kg feed, respectively, while in T5, T6, and T7 0.5g, 1.0g and 2.0g/kg feed of prebiotic (mannonoligosacchride of Saccharomyces cell wall-47g, formic acid-32g) was supplemented, respectively. The percent hen day egg production and feed efficiency (kg/dozen eggs and kg/kg egg mass) were improved (P<0.05) in probiotic and prebiotic incorporated diets. The mRNA expression levels of Toll like receptors (TLRs) TLR2 and TLR4 was increased (P<0.05) when graded levels of the probiotic and prebiotic were supplemented, while, TLR7 did not show any significant change. Thus, it can be inferred that the inclusion of the probiotic and prebiotic could enhance the production performance and immune status of birds by augmenting the T cell mediated immune response.

REFERENCES

  1. Ansari, A.R., Wen, L., Huang, H.B., Wang, J.X., Huang, X.Y., Peng, K.M and Liu, H.Z. (2015). Lipopolysacchride stimulation upregulated Toll-like receptor 4 expression in chicken cerebellum. Vet. Immunol. Immunopathol. 166 (3-4): 145-150.
  2. Bureau of Indian Standards, BIS. (2007). Poultry Feeds –specification. 5th review. IS: 1374-2007, Manak Bhawan, 9 Bahadurshah Zafar Marg, New Delhi. 
  3. Cervantes, H. (2006). Banning antibiotic growth promoters: Learning from the European experience. Poult. Int. 45: 14-15. 
  4. Clancy, R. (2003). Immunobiotics and the probiotic evolution, FEMS. Immunol. Med. Microbio. 38: 9–12. 
  5. Dowarah, R., Verma, A.K., Agrawal, Neeta, Singh, P. (2016).Effect of swine-based probiotic on growth performance, nutrient utilization and immune status of early-weaned grower-finisher crossbred pigs. Anim. Nutri. Feed Techn. 16(3): 451-461. 
  6. Erickson, K.L. and Hubbard, N.E. (2000). Probiotic immunomodulation in health and disease. J. Nutr. 130: 403S-409S. 
  7. Gholap, S.S., Patil, M.B., Kank, V.D., Ranade, A.S., Gadegaonkar, G.M., Gulavane, S. U., Karambele, N.R. (2009). Studies on effects of prebiotics (MOS) as growth promoter in broilers. Journal of Bombay Veterinary College, 17(1): 63-65. 
  8. Hajati, H., Hassanabadi, A., and Teimouri Yansari, A. (2014). The effect of dietary supplementation of prebiotic and probiotic on performance, humoral immunity responses and egg hatchability in broiler breeders. Poult. Sci. 2(1): 1-13. 
  9. Hajati, H. and Rezaei, M. (2010). The application of prebiotics in poultry production. Inter. J. Poult. Sci. 9: 298-304. 
  10. Higgins, S.E., Higgins, J.P., Wolfenden, A.D., Henderson, S.N., Torres-Rodriguez, A., Tellez Gand Hargis, B. (2010). Chicken toll-like receptors and their role in immunity. World’s Poult. Sci. Assoc. 66: 727–738.
  11. Lei, Y., Zhang, K.Y., Ding, X.M., Bai, S.P. and Jung, S.C. (2009). Effect of probiotics on growth performance, development of small intestinal tract and microbial populations in broilers. J. Agric. Sci. Technol. 3: 24–31. 
  12. Livak, K.J., Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25(4): 402-8.
  13. SPSS statistics version 21 for windows 7. Propery of IBM corporation, 2012.
  14. Sato, M., Sano, H., Iwaki, D., Kudo, K., Konishi, M., Takahashi, H., Takahashi, T., Imaizumi, H., Asai, Y. and Kuroki, Y. (2003). Direct binding of Toll-like receptor 2 to zymosan, and zymosan-induced NF-êB activation and TNF-a secretion are down-regulated by lung collection surfactant protein A. J. Immunol. 171: 417–425. 
  15. Sato, K., Takahashi, K., Tohno, M., Miura, Y., Kamada, T., Ikegami, S. and Kitazawa, H. (2009). Immunodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets. Poult. Sci. 88: 2532–2538. 
  16. Stanley, V.G., Brown, C. and Sefton, T. (2000). Single and combined effects of dietary protease and mannan oligosaccharide on the performance of laying hens. Poult. Sci. 79: 62 (Abstract). 
  17. Swain, B.K. Naik, P.K, Chakurkar, E.B., Singh, N.P. (2016). Effect of supplementation of probiotics on the performance of Vanaraja Chicks. Indian J. Anim. Nutr. 33(3): 353-356. 
  18. Tohno, M. et al. (2007). Advanced molecular immunoassay system for immunobiotic lactic acid bacteria using a transfectant of Toll-like receptor 2. Anim. Sci. J. 78: 195–205. 
  19. Tuohy, K.M., Rouzaud, G.C., Brück, W.M. and Gibson, G.R. (2005). Modulation of the human gut microflora towards improved health using prebiotic-assessment of efficacy. Current Pharmaceutical Design, 11: 75- 90. 
  20. Uematsu, S. and Akira, S. (2008). Toll-like receptors (TLRs) and their ligands. Handb. Exp. Pharmacol. 183: 1–20. 
  21. Yurong, Y., Ruiping, S., Shimin, Z. and Yibao, J. (2005). Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens. Arch. Anim. Nutr. 59: 237-246. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)