Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 11 (november 2018) : 1537-1542

Cloning, characterization and identification of polymorphism in TCR Zeta Gene in deoni cattle

K. Swathi, M. Gnana Prakash, D. Sakaram, T. Raghunandan, A. Sarat Chandra, P. Kalyani
1Department of Animal Genetics and Breeding, College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Hyderabad- 500 030, Telangana, India.
Cite article:- Swathi K., Prakash Gnana M., Sakaram D., Raghunandan T., Chandra Sarat A., Kalyani P. (2018). Cloning, characterization and identification of polymorphism in TCR Zeta Gene in deoni cattle. Indian Journal of Animal Research. 52(11): 1537-1542. doi: 10.18805/ijar.B-3389.

ABSTRACT

The cDNA encoding, T-cell receptor zeta (TCR z; CD247) molecule of Deoni cattle (Bos indicus), was isolated, cloned and sequenced in the present study. The CD247 cDNA comprised 1078 nucleotides including a 30 nucleotide 5¹-untranslated region (UTR), 495 nucleotide single open reading frame (ORF) and 553 nucleotide 3¹-UTR. Deduced amino acid of cattle CD247 sequence was two residues shorter than the corresponding sheep sequences. However, ruminant-specific insertions and substitutions in transmembrane (TM) and intra-cytoplasmic (IC) domain were present in cattle. Immunoreceptor tyrosine-based activation motifs (ITAMs), the important motifs for TCR signalling, were totally conserved among ruminants including cattle. The 3¹ - UTR region of the cattle CD247 was highly homologous to the corresponding region in the buffalo sequence and showed lack of polymorphism after polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using Hae III and Mse I restriction enzymes in cattle population. Phylogenetically, cattle sequence was closer to buffalo sequence under the ruminant’s lineage. The conserved nature of this gene ensures TCR integrity which is vital for induction of optimal and efficient immune response.

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