Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 50 issue 2 (april 2016) : 215-217

Immunogenicity of human umbilical cord mesenchymal stem cells during ex vivo expansion

Mingli Ji*, LiPing Guan1
1<p>Department of Physiology, Xinxiang Medical College,&nbsp;453003 Xinxiang, China.</p>
Cite article:- Ji* Mingli, Guan1 LiPing (2016). Immunogenicity of human umbilical cord mesenchymalstem cells during ex vivo expansion . Indian Journal of Animal Research. 50(2): 215-217. doi: 10.18805/ijar.9541.

ABSTRACT

The aim of this study was to investigate the immunogenicity of human umbilical cord mesenchymal stem cells (hUCMSCs) cultured in vitro by detecting changes in major histocompatibility complex expression. Major histocompatibility complex expression was detected by RT–PCR and flow cytometry. Morphologically, hUCMSCs exhibited no visible changes before the 5 st passage of the in vitro culture. In addition, At the molecular level, the cells continued expressing the specific positive surface markers, CD105, CD73 and CD90, and did not express the negative surface markers, CD14, CD34 or CD45, during culture ex vivo. Furthermore, the hUCMSCs exhibited low immunogenicity, which was maintained when cultured for five passages. Taken as a whole, these results suggested that hUCMSCs cultured in vitro may be safely for transplanted because of low immunogenicity before the fifth passage. 

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