Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 6 (december 2017) : 1066-1072

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Tarun Kumar Varun, Swaraj Senani, Narender Kumar, Mayank Gautam, Ritika Gupta, Mokshata Gupta
1<p>Department of Animal Nutrition, ICAR-National Institute of Animal Nutrition and Physiology,&nbsp;Adugodi, Bangalore-560 030, Karnataka, India.</p>
Cite article:- Varun Kumar Tarun, Senani Swaraj, Kumar Narender, Gautam Mayank, Gupta Ritika, Gupta Mokshata (2017). Extraction and characterization of chitin, chitosan and chitooligosaccharidesfrom crab shell waste . Indian Journal of Animal Research. 51(6): 1066-1072. doi: 10.18805/ijar.v0iOF.8456.

ABSTRACT

An experiment was carried out to utilize the crab shell waste for the production of chitin by chemical extraction method. The obtained chitin was processed to make the more useful compound chitosan by deacetylation of chitin using strong NaOH solution at high temperature. Yield of chitin and chitosan from crab shell waste was found to be 12.2 % and 10.54 %, respectively. Chitosan was further used to produce chito-oligosaccharides (COS) by acid hydrolysis using 0.5 N H2SO4, 1N H2SO4 and 7N HCl at hourly intervals upto 6 hours and the average yield was 0.55, 0.84 and 1.93 mg/ml, respectively. Chitin and chitosan were characterized by Fourier Transform Infrared spectroscopy (FT-IR) technique which depicts different band representing various bonds present in the molecule and which were in agreement with the bands of standard chitin and chitosan. Further, COS were formed after acid hydrolysis of chitosan, which was characterized by Thin Layer Chromatography (TLC) using glucosamine as a standard. 

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