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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 6 (june 2018) : 907-910,   Doi: 10.18805/ijar.B-3298

Molecular characterization of Staphylococcus spp. isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur, India

S
Sharmita Doley
V
V. C. Ingle
P
P. A. Tembhurne
S
S. R. Warke
P
Prasanna Pati
N
Nekibuddin Ahmed
1Department of Veterinary Microbiology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur-440006, Maharashtra, India
Cite article:- Doley Sharmita, Ingle C. V., Tembhurne A. P., Warke R. S., Pati Prasanna, Ahmed Nekibuddin (2017). Molecular characterization of Staphylococcus spp. isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur, India. Indian Journal of Animal Research. 52(6): 907-910. doi: 10.18805/ijar.B-3298.

ABSTRACT

Present study was undertaken to characterize Staphylococcus spp. associated with respiratory tract infection in small ruminants. A total of 139 bacterial isolates were recovered from clinically sick and apparently healthy small ruminants. The prevalence rate of Staphylococcus spp. in sick and healthy animals was 44.94 and 24% respectively. The sensitivity pattern of selected antibiotics was similar in both clinically sick and apparently healthy animals except streptomycin and penicillin-G.  PCR assay was standardized for the detection of pathogenic genes of Staphylococcus spp. viz. clfA and spa. Among 34 random isolates selected, the prevalence rate of clfA and spa gene  was 79.41 and 58.82% respectively indicating their pathogenic potential. In conclusion, Staphylococcus spp. was found to be one of the highly prevalent organism in respiratory tract infections.

REFERENCES

  1. Aher, T., Roy, A. and Kumar, P. (2012). Molecular detection of virulence genes associated with pathogenicity of Klebsiella spp. isolated from the respiratory tract of apparently healthy as well as sick Goats. Israel J. Vet. Med., 67(4): 249-252.
  2. Asaduzzaman, M., Ahmed, K.S., Khan, M.F.R., Nazmul, K.H.M.H.N and Mostafizur, R.M. (2013). Isolation and identification of bacteria from upper respiratory tract of Black Bengal goat in Bangladesh and investigation of some epidemiological parameters related to pneumonia. Sci. J. Microbiol., 2(11) 207-213.
  3. Bauer, A.W., Kirby, W.M.M., Shernis, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by standardized single disc method. American J. Clin. Pathol.45: 493-496.
  4. Cowan, S.T. and Steel, K.J. (1993). Manual for the Identification of Medical Bacteria. The Syndics of the Cambridge University Press. Bentlay House, 200 Euston Road, London, U.K.
  5. Cruickshank, R., Duguid, J.P., Marmoin, B.P. and Swain, R.H.A. (1975). Medical Microbiology Vol. 2, 12th edn. Churchill Livingstone, Edinburgh, London and New York.
  6. Karahan, M., Acik, M.N. and Cetinkaya, B. (2011). Investigation of virulence genes by PCR in Staphylococcus aureus isolates originated from subclinical bovine mastitis in Turkey. Pakistan Vet. J., 31(3):249-253. 
  7. Lacasta, D., Ferrer, L.M., Ramos, J. J., Gonz´alez, J. M. and De las Heras. M. (2008). Influence of climatic factors on the development of Pneumonia in lambs, Small Ruminant Res., 80(1–3): 28–32.
  8. Martin, W.B. (1996). Respiratory infections of sheep. Comp. Immun. Microbial Infect-Dis. 19: 171-179.
  9. Megra, T., Sisay, T. and Asseged, B. (2006). The aerobic bacterial flora of the respiratory passageways of healthy goats in Dire Dawa Abattoir, Eastern Ethiopia. Revue Méd. Vét. 157(2): 84-87.
  10. Momtaz, H., Rahimi, E. and Tajbakhsh, E. (2010). Detection of some virulence factors in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran. African J. Biotech., 9(25): 3753-3758.
  11. Quinn, P.J., Carter, M.E., Markey, B. and Carter, G.R. (1994). Clinical Veterinary Microbiology. Walfe publishing, an imprint of Mosby year book Europe Limited.
  12. Seki, K., Sakurada, J., Seong, H.K., Murai, M., Tachi, H., Ishii, H. and Masuda, S. (1998). Occurrence of coagulase serotype among S. aureus strains isolated from healthy individuals with special reference to correlation with size of protein-A gene. Microbiol. Immunol., 42: 407-409.
  13. Stephan, R., Annemuller, C., Hasan, A.A. and Lammler, C.H. (2001). Characterization of enterotoxigenic S. aureus strains isolated from bovine mastitis in north-east Switzerland. Vet. Microbiol., 78: 373-382.
  14. Wilson, K. (1987) Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, 2.4.1-2.4.5. Edited by Ausubel, F.M., Brent, R., Kingston, R. E., Moore, D.D., Seidman, J. G., Smith, J.A. and Struhl, K, Green Publishing and Wiley-Inter science. New York,
  15. Woldemeskel, M., Tibbo, M. and Potgieter, L.N.D. (2002). “Ovine progressive pneumonia(Maedi-Visna): an emerging respiratory disease of sheep in Ethiopia, Deutsche Tierarztliche Wochenschrift, 109(11): 486–488.
  16. Yesuf, M., Hailu, M. and Chanie, M. (2012). Histopathological and Bacteriological examination of pneumonic lungs of small Ruminants slaughtered at Gondar, Ethiopia. American-Eurasian J. Sci. Res., 7(6): 226-231.
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    Research Article

    volume 52 issue 6 (june 2018) : 907-910,   Doi: 10.18805/ijar.B-3298

    Molecular characterization of Staphylococcus spp. isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur, India

    S
    Sharmita Doley
    V
    V. C. Ingle
    P
    P. A. Tembhurne
    S
    S. R. Warke
    P
    Prasanna Pati
    N
    Nekibuddin Ahmed
    1Department of Veterinary Microbiology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur-440006, Maharashtra, India
    Cite article:- Doley Sharmita, Ingle C. V., Tembhurne A. P., Warke R. S., Pati Prasanna, Ahmed Nekibuddin (2017). Molecular characterization of Staphylococcus spp. isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur, India. Indian Journal of Animal Research. 52(6): 907-910. doi: 10.18805/ijar.B-3298.

    ABSTRACT

    Present study was undertaken to characterize Staphylococcus spp. associated with respiratory tract infection in small ruminants. A total of 139 bacterial isolates were recovered from clinically sick and apparently healthy small ruminants. The prevalence rate of Staphylococcus spp. in sick and healthy animals was 44.94 and 24% respectively. The sensitivity pattern of selected antibiotics was similar in both clinically sick and apparently healthy animals except streptomycin and penicillin-G.  PCR assay was standardized for the detection of pathogenic genes of Staphylococcus spp. viz. clfA and spa. Among 34 random isolates selected, the prevalence rate of clfA and spa gene  was 79.41 and 58.82% respectively indicating their pathogenic potential. In conclusion, Staphylococcus spp. was found to be one of the highly prevalent organism in respiratory tract infections.

    REFERENCES

    1. Aher, T., Roy, A. and Kumar, P. (2012). Molecular detection of virulence genes associated with pathogenicity of Klebsiella spp. isolated from the respiratory tract of apparently healthy as well as sick Goats. Israel J. Vet. Med., 67(4): 249-252.
    2. Asaduzzaman, M., Ahmed, K.S., Khan, M.F.R., Nazmul, K.H.M.H.N and Mostafizur, R.M. (2013). Isolation and identification of bacteria from upper respiratory tract of Black Bengal goat in Bangladesh and investigation of some epidemiological parameters related to pneumonia. Sci. J. Microbiol., 2(11) 207-213.
    3. Bauer, A.W., Kirby, W.M.M., Shernis, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by standardized single disc method. American J. Clin. Pathol.45: 493-496.
    4. Cowan, S.T. and Steel, K.J. (1993). Manual for the Identification of Medical Bacteria. The Syndics of the Cambridge University Press. Bentlay House, 200 Euston Road, London, U.K.
    5. Cruickshank, R., Duguid, J.P., Marmoin, B.P. and Swain, R.H.A. (1975). Medical Microbiology Vol. 2, 12th edn. Churchill Livingstone, Edinburgh, London and New York.
    6. Karahan, M., Acik, M.N. and Cetinkaya, B. (2011). Investigation of virulence genes by PCR in Staphylococcus aureus isolates originated from subclinical bovine mastitis in Turkey. Pakistan Vet. J., 31(3):249-253. 
    7. Lacasta, D., Ferrer, L.M., Ramos, J. J., Gonz´alez, J. M. and De las Heras. M. (2008). Influence of climatic factors on the development of Pneumonia in lambs, Small Ruminant Res., 80(1–3): 28–32.
    8. Martin, W.B. (1996). Respiratory infections of sheep. Comp. Immun. Microbial Infect-Dis. 19: 171-179.
    9. Megra, T., Sisay, T. and Asseged, B. (2006). The aerobic bacterial flora of the respiratory passageways of healthy goats in Dire Dawa Abattoir, Eastern Ethiopia. Revue Méd. Vét. 157(2): 84-87.
    10. Momtaz, H., Rahimi, E. and Tajbakhsh, E. (2010). Detection of some virulence factors in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran. African J. Biotech., 9(25): 3753-3758.
    11. Quinn, P.J., Carter, M.E., Markey, B. and Carter, G.R. (1994). Clinical Veterinary Microbiology. Walfe publishing, an imprint of Mosby year book Europe Limited.
    12. Seki, K., Sakurada, J., Seong, H.K., Murai, M., Tachi, H., Ishii, H. and Masuda, S. (1998). Occurrence of coagulase serotype among S. aureus strains isolated from healthy individuals with special reference to correlation with size of protein-A gene. Microbiol. Immunol., 42: 407-409.
    13. Stephan, R., Annemuller, C., Hasan, A.A. and Lammler, C.H. (2001). Characterization of enterotoxigenic S. aureus strains isolated from bovine mastitis in north-east Switzerland. Vet. Microbiol., 78: 373-382.
    14. Wilson, K. (1987) Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, 2.4.1-2.4.5. Edited by Ausubel, F.M., Brent, R., Kingston, R. E., Moore, D.D., Seidman, J. G., Smith, J.A. and Struhl, K, Green Publishing and Wiley-Inter science. New York,
    15. Woldemeskel, M., Tibbo, M. and Potgieter, L.N.D. (2002). “Ovine progressive pneumonia(Maedi-Visna): an emerging respiratory disease of sheep in Ethiopia, Deutsche Tierarztliche Wochenschrift, 109(11): 486–488.
    16. Yesuf, M., Hailu, M. and Chanie, M. (2012). Histopathological and Bacteriological examination of pneumonic lungs of small Ruminants slaughtered at Gondar, Ethiopia. American-Eurasian J. Sci. Res., 7(6): 226-231.
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