Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 52 issue 1 (january 2018) : 25-28

Characteristics and freezability of Assam Hill goat semen

S. Deori, B.C. Deka, R.K. Biswas, N. Nahardeka, A. Arangasamy, D. Bhuyan, D.J. Kalita, R.S. Borah, Arundhati Phookan
1<p>College of Veterinary Science, Assam Agricultural University, Khanapara,&nbsp;Guwahati-781022, Assam,&nbsp;India</p>
Cite article:- Deori S., Deka B.C., Biswas R.K., Nahardeka N., Arangasamy A., Bhuyan D., Kalita D.J., Borah R.S., Phookan Arundhati (2017). Characteristics and freezability of Assam Hill goat semen . Indian Journal of Animal Research. 52(1): 25-28. doi: 10.18805/ijar.v0iOF.7821.

Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The  mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.


  1. Akela, A. (2006). Seasonal variation in semen characteristics of Assam Local and Beetal bucks. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati, India.

  2. Blom, E. (1977). Sperm morphology with reference to bull fertility. First All India Symposium on Animal Reproduction. pp. 61-81. Ludhiana, India 

  3. Borgohain, A. C. (1981). Studies on semen characteristics and fertility of Assam Local and Beetal bucks. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati, India.

  4. Jeyendran, R. S., Van-der-Ven, H. H., Perez-Pelaez, M., Crabo, B. G. and Zaneveld, L. J. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fert. 70: 219-228.

  5. Khalili, B., Farshad, A., Zamiri, M. J., Rashidi, A. and Fazeli, P. (2009). Effects of sucrose and trehalose on the freezability of Markhoz goat spermatozoa. Asian- Aus. J. Anim. Sci. 22: 1614-1619. 

  6. Gangwar, C., Ranjan, R., Kumar, S., Kharche, S. D., Goel, A. K., Ramachandran, N. and Jindal, S. K. (2014). Use of chelating agent for optimum post thaw semen quality of buck semen. Indian J. Anim. Sci. 84: 839-841.

  7. Lalramdintluanga (2012). Effect of additives on quality of frozen goat semen. M.V.Sc Thesis, Assam Agricultural University, Guwahati, Assam, India. 

  8. Mahto, D., Singh, L. B., Sinha, M. P., Singh, D. K. and Jha, D. K. (2012). Study of seminal attributes and fertility in Black Bengal goat. XXVIII Annual Convention of Indian Society of Animal Reproduction. 21-23 November, Guwahati, India.

  9. Pradhan, M. G. A., Rahman, M. S., Kwon, W. S., Mishra, D., Kamal, M. M., Bhuiyan, M. M. U. and Shamsuddin, M. (2013). Duration of preservation affect the quality of chilled Black Bengal buck semen. J. Emb. Tran. 28: 113-119.

  10. Ranjan, R., Goel, A. K., Ramachandran, N., Kharche, S. D. and Jindal, S. K. (2015). Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian J. Small Rum. 21: 32–36.

  11. Saikia, P. (2006). Studies on freezing of semen of Assam Local and Beetal goats. M.V.Sc Thesis, Assam Agricultural University, Khanapara, Guwahati, India.

  12. Sarma, J. P., Sinha, S., Biswas, R. K., Deka, B. C., Sarmah, B. C. and Gogoi, T. (2011). Physical characteristics of Beetal goat semen. XXVII Annual Convention of Indian Society for Study of Animal Reproduction. 27-29 September, Aizawl, India. 

  13. Sinha, S. (1989). Effect of extenders, glycerol levels and equilibration periods on quality of frozen goat semen. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati, India.

  14. Watson, P. F. (1975). Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Rec. 97: 12–15. 

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