Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 51 issue 2 (april 2017) : 231-234

Genetic polymorphism of growth hormone releasing hormone gene in exotic and crossbred pigs

Purabi Kaushik, Soumen Naskar, Pratap J. Handique, Habibur Rahman, Dilip K. Sarma
1<p>ICAR-National Research Centre on Pig, Rani,&nbsp;Guwahati-781 014, Assam, India.</p>
Cite article:- Kaushik Purabi, Naskar Soumen, Handique J. Pratap, Rahman Habibur, Sarma K. Dilip (2016). Genetic polymorphism of growth hormone releasing hormonegene in exotic and crossbred pigs . Indian Journal of Animal Research. 51(2): 231-234. doi: 10.18805/ijar.11162.

ABSTRACT

Growth hormone releasing hormone (GHRH) plays a central role in growth and production through its influence on important metabolic activities in mammals which prompted its wide commercial use including use as a candidate marker. In present experiment, polymorphism at exon 2 and 3 of Growth Hormone Releasing Hormone gene (GHRH) was studied using PCR–RFLP in crossbred and exotic pigs, commonly found in north east India. Restriction enzyme digestion of 467 bp  PCR amplicon with AluI revealed 1 genotype. Comparison of obtained sequences revealed large scale nucleotide and residue substitutions. Observed polymorphism may be associated with wide variability in growth (rate) of different breeds of pig.

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