Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
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Novel SNPs in ATP1B2 Gene and their Association with Heat Tolerance Indicator Traits in Sahiwal Cattle
First Online 19-10-2015|
Heat Shock Proteins (HSPs) are expressed in response to heat stress and the polymorphism in HSP genes at single nucleotide level is reported to be associated with heat tolerance and production performance traits in cattle. ATP1B2 gene was mapped to Bos taurus autosome 19 (BTA 19) spanning nearly 4310bp, comprising 7 Exons and 6 Introns. The present study was conducted in Sahiwal cows (n=108) raised under sub-tropical climate with the objectives to identify SNPs in targeted regions (Intron 2, 4 and Exon 3, 5) of ATP1B2 gene and to analyze their association with heat tolerance traits in Sahiwal cows. Respiration rate (RR) and rectal temperature (RT) were recorded during probable extreme hours in winter, spring and summer seasons. Heat tolerance coefficient (HTC) was also calculated to check the adaptability of the animals during the period of heat stress. The comparative sequence analysis revealed a total of two SNPs in Intron 2 of ATP1B2 gene i.e. g.2243G>A and g.2366T>C. Out of these two identified SNPs, only one SNP i.e. g.2243G>A was found to be significantly (PC, RR, RT and HTC were having non-significant association with the different genotypes i.e. TT and TC. These findings suggest that AA and GA genotype of SNP g.2243G>A of ATP1B2 gene can be utilized as potential marker for propagating thermo-tolerant cattle.
- Beatty, D.T., Barnes, A., Taylor, E., Pethick, D., McCarthy, M and Maloney, S.K. (2006). Physiological responses of B. taurus and B. indicus cattle to prolonged, continuous heat and humidity. Journal of Animal Science., 84:972–985
- Benezra, M.V. (1954). A new index for measures the adaptability of cattle to tropical condition. Proc. J. Anim. Sci., 13:1015
- Berry, I.L., Shanklin, M.D. and Johnson, H.D. (1964). Dairy shelter design based on milk production decline as affected by temperature and humidity. Trans. Am. Soc. Ag. Eng, 7: 329-331
- Collier, R.J., Collier, J.L., Rhoads, R.P. and Baumgard, L.H. (2008). Invited Review: Genes involved in the bovine heat stress response. J. Dairy Sci., 91:445–454
- Das, R., Gupta I.D., Verma, A., Singh, A., Chaudhari, M.V., Sailo, L., Upadhyay, R.C. and Goswami, J. (2015). Genetic polymorphisms in ATP1A1 gene and their association with heat tolerance in Jersey crossbred cows. Indian Journal of dairy Science., 68:50-54
- Liu, Y., Li, D., Huixia., Li, H., Zhou, X. and Wang, G. (2011). A novel SNP of the ATP1A gene is associated with heat tolerance traits in dairy cows. Mol. Biol. Rep., 38: 83-88
- McDowell, R. E., Hooven, N. W. Adcamoens, J. K. (1976). Effects of climate on performance of Holsteins in first lactation. J. Dairy Sci., 59: 965–973
- National Research Council. (1971). A Guide to Environmental Research on Animals. Natl. Acad. Sci. Washington, DC.
- Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual 3rd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
- Thom, E.C. (1958). Cooling degree days. Air Conditioning, Heating and Ventilating., 55:65-69
- Vague, P., Coste, T.C., Jannot, M.F., Raccah, D and Tsimaratos, M. (2004) C-peptide, Na+-K+-ATPase and Diabetes. Exp Diabesity Res., 5:37–50
- Wang, Z., Wang, Genlin., Huang, J., Li, Q., Wang, C. and Zhong, J.(2011).Novel SNPs in the ATP1B2 gene and their associations with milk yield, milk composition and heat- resistance traits in Chinese Holstein cows. Molecular Biology Reports., 38:1749-1755
- Yang, S. (2007). Study on seasonal variation of some biochemical indices of blood and correlation between them and heat tolerance in Holstein cows. Xinjiang Agric Univ.,28–35
- Yeh, F. C., Yang, R. C. and Boyle, T. (1999). POPGENE VERSION 1.31: Microsoft Window-based free Software for Population Genetic Analysis, ftp://ftp.microsoft.com/Softlib/HPGL.EXE.
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